Flaconitine (Acetylaconitine)
(Synonyms: 乙酰乌头碱,Acetylaconitine; 3-Acetylaconitine) 目录号 : GC33717
A diterpene alkaloid with diverse biological activities
Cas No.:77181-26-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | The assay is performed following a standard published procedure using microtiter plate format and sulforhodamine B standing at the end point. The leukemic cells (P388) are exposed to Flaconitine (3-Acetylaconitine) for three days. Cells are grown and maintained in an incubator set at 37°C, 5% CO2 and 95% humidity. RPMI-1640 media supplemented with 10% fetal calf serum (FCS) and 0.5% trypsin are used to culture P388 cells[2]. |
References: [1]. Dong L, et al. Identification of nuclear factor-κB inhibitors and β2 adrenergic receptor agonists in Chinese medicinal preparation Fuzilizhong pills using UPLC with quadrupole time-of-flight MS. Phytochem Anal. 2014 Mar-Apr;25(2):113-21. | |
3-Acetylaconitine is a diterpene alkaloid that has been found in Aconitum and has diverse biological activities.1,2 It is cytotoxic to P388 leukemia cells (IC50 = 18.6 ?M).1 It inhibits brevetoxin binding to (IC50 = 1.3 ?M), and increases free sodium and calcium concentrations in, rat brain synaptosomes. 3-Acetylaconitine (60-100 nM) induces arrhythmia in isolated guinea pig atria.2 In vivo, 3-acetylaconitine (100 ?g/kg, i.v.) reduces paw licking, flicking, and shaking in a mouse model of formalin-induced inflammatory pain.
1.He, Y.-Q., Yao, B.-H., and Ma, Z.-Y.Diterpenoid alkaloids from a Tibetan medicinal plant Aconitum richardsonianum var. pseudosessiliflorum and their cytotoxic activityJ. Pharm. Anal.1(1)57-59(2011) 2.Gutser, U.T., Friese, J., Heubach, J.F., et al.Mode of antinociceptive and toxic action of alkaloids of Aconitum specNaunyn Schmiedebergs Arch. Pharmacol.375(1)39-48(1997)
| Cas No. | 77181-26-1 | SDF | |
| 别名 | 乙酰乌头碱,Acetylaconitine; 3-Acetylaconitine | ||
| Canonical SMILES | CO[C@@H]1C2([C@@](C[C@@]3(O)[C@@H]4OC(C5=CC=CC=C5)=O)([H])[C@@]4([H])[C@](OC(C)=O)([C@@H](O)[C@@H]3OC)C6C2N(CC)C7)[C@@]([C@H]6OC)([H])[C@@]7(COC)[C@H](OC(C)=O)C1 | ||
| 分子式 | C36H49NO12 | 分子量 | 687.77 |
| 溶解度 | DMSO : 50 mg/mL (72.70 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.454 mL | 7.2699 mL | 14.5397 mL |
| 5 mM | 0.2908 mL | 1.454 mL | 2.9079 mL |
| 10 mM | 0.1454 mL | 0.727 mL | 1.454 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。