Flagelin 22 (Flagellin 22)
(Synonyms: Flagellin 22) 目录号 : GC32202
Flagelin 22 (Flagellin 22)是细菌鞭毛蛋白的片段，是植物和藻类的有效诱导剂。
Cas No.:304642-91-9
Sample solution is provided at 25 µL, 10mM.
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Flagelin 22 (Flagellin 22), a fragment of bacterial flagellin, is an effective elicitor in both plants and algae[1].
Flagelin 22 is a 22-amino-acid peptide, which corresponds to the highly conserved N-terminal region of flagellin, can induce immunity reaction in various plants such as tomato , potato , tobacco and Arabidopsis thaliana. Moreover, Flagelin 22 significantly induces growth inhibition of algae at a concentration of 1 μM[1]. Treatment of tobacco cells with 200 nMFlagelin 22 induced the production of 13 metabolites. Compared with other microbial-associated molecular pattern (MAMP) molecules and plant hormones, Flagelin 22-treated cells produced a unique metabolite, feruloyltyrosine glycoside isomer 3 [2]. Flagelin 22 (100 nM) activates triggered immunity (PTI) in plants leading to a decrease in the expression of genes involved in the biosynthesis of the growth-promoting hormone brassinosteroid (BR)[3].
References:
[1] Bojun Lu, et al. Defense responses in female gametophytes of Saccharina japonica (Phaeophyta) induced by flg22-derived peptides. Journal of Applied Phycology (2016), 28(3), 1793-1801.
[2] Mhlongo M I, Piater L A, Madala N E, et al. Phenylpropanoid defences in Nicotiana tabacum cells: Overlapping metabolomes indicate common aspects to priming responses induced by lipopolysaccharides, chitosan and flagellin-22[J]. PLoS One, 2016, 11(3): e0151350.
[3] Jiménez-Góngora T, Kim SK, Lozano-Durán R, Zipfel C. Flg22-Triggered Immunity Negatively Regulates Key BR Biosynthetic Genes. Front Plant Sci. 2015 Nov 9;6:981.
Flagelin 22 (Flagellin 22)是细菌鞭毛蛋白的片段，是植物和藻类的有效诱导剂[1]。
Flagelin 22是一种由22个氨基酸组成的肽，对应于鞭毛蛋白高度保守的N端区域，可在番茄、马铃薯、烟草和拟南芥等多种植物中诱导免疫反应，并且，Flagelin 22在1μM的浓度下显著诱导藻类的生长抑制[1]。使用200 nM的Flagelin 22处理烟草细胞可诱导产生13种代谢物，相较于其他微生物相关分子模式（MAMP）分子和植物激素，Flagelin 22处理的细胞会产生一种独特的代谢物阿魏酰酪胺糖苷异构体3[2]。Flagelin 22（100 nM）可激活植物中的触发免疫（PTI）导致生长促进激素油菜素内酯（BR）生物合成基因的表达减少[3]。
| Cell experiment [1]: | |
Cell lines | Tobacco cell |
Preparation Method | Tobacco cells were induced with MAMPs (namely lipopolysaccharides, chitosan, and Flagelin 22) and phytohormones (abscisic acid , methyljasmonic acid, and methyljasmonic acid) and the cells were harvested at different times to extract metabolites. |
Reaction Conditions | 200 nM, 0、6、12、18 and 24h |
Applications | Flagelin 22 cells induced the production of 13 metabolites, and compared with other elicitors, Flagelin 22 cells had a unique metabolite Feruloyltyramine glycoside isomer 3. |
References: [1] Mhlongo M I, Piater L A, Madala N E, et al. Phenylpropanoid defences in Nicotiana tabacum cells: Overlapping metabolomes indicate common aspects to priming responses induced by lipopolysaccharides, chitosan and flagellin-22[J]. PLoS One, 2016, 11(3): e0151350. | |
| Cas No. | 304642-91-9 | SDF | |
| 别名 | Flagellin 22 | ||
| Canonical SMILES | Gln-Arg-Leu-Ser-Thr-Gly-Ser-Arg-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala | ||
| 分子式 | C93H162N32O34 | 分子量 | 2272.48 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.44 mL | 2.2002 mL | 4.4005 mL |
| 5 mM | 0.088 mL | 0.44 mL | 0.8801 mL |
| 10 mM | 0.044 mL | 0.22 mL | 0.44 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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