FlAsH-EDT2
(Synonyms: 荧光含砷螺旋粘结剂/卢米奥绿色,Fluorescein Arsenical Helix Binder, Lumio Green) 目录号 : GC43674A fluorescent probe that tags proteins in cells
Cas No.:212118-77-9
Sample solution is provided at 25 µL, 10mM.
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FlAsH-EDT2 is a pro-fluorescent, membrane-permeable biarsenical compound that binds covalently to a tetracysteine sequence (CCPGCC), which is engineered into target proteins.[1],[2] It binds proteins that have the CCPGCC tag almost immediately after translation.[2] FlAsH-EDT2 is commonly used to study protein trafficking, folding, and interactions in living cells or cell lysates.[2],[3],[4] This green-emitting fluorophore is excited at 508 nm, with emission at 528 nm.[4]
Reference:
[1]. Griffin, B.A., Adams, S.R., and Tsien, R.Y. Specific covalent labeling of recombinant protein molecules inside live cells. Science 287(5374), 269-272 (1998).
[2]. Rudner, L., Nydegger, S., Coren.L.V., et al. Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling. J.Virol. 79(7), 4055-4065 (2005).
[3]. Luedtke, N.W., Dexter, R.J., Fried, D.B., et al. Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH. Nat.Chem.Biol. 3(12), 779-784 (2007).
[4]. Perdios, L., Bunney.T.D., Warren, S.C., et al. Time-resolved FRET reports FGFR1 dimerization and formation of a complex with its effector PLCγ1. Adv.Biol.Regul. 60, 6-13 (2016).
| Cas No. | 212118-77-9 | SDF | |
| 别名 | 荧光含砷螺旋粘结剂/卢米奥绿色,Fluorescein Arsenical Helix Binder, Lumio Green | ||
| 化学名 | 4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one | ||
| Canonical SMILES | OC1=C([As]2SCCS2)C3=C(C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C([As]7SCCS7)=C6O3)C=C1 | ||
| 分子式 | C24H18As2O5S4 | 分子量 | 664.5 |
| 溶解度 | DMF: 15 mg/ml,DMSO: 20 mg/ml,DMSO:PBS (pH 7.2) (1:10): 0.09 mg/ml | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5049 mL | 7.5245 mL | 15.0489 mL |
| 5 mM | 0.301 mL | 1.5049 mL | 3.0098 mL |
| 10 mM | 0.1505 mL | 0.7524 mL | 1.5049 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet