Fluorene
(Synonyms: 芴) 目录号 : GC61429
Fluorene是一种多环芳烃(PAH)，是其他芴类化合物的前体。Fluorene及其衍生物可作为芴的染料前体。
Cas No.:86-73-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fluorene, a polycyclic aromatic hydrocarbon (PAH), is a precursor to other fluorene compounds. Fluorene and its derivative can be used as a precursor to fluorene-based dyes[1].
[1]. Paul Olusegun Bankole, et al. Biodegradation of fluorene by the newly isolated marine-derived fungus, Mucor irregularis strain bpo1 using response surface methodology. Ecotoxicol Environ Saf. 2021 Jan 15;208:111619.
| Cas No. | 86-73-7 | SDF | |
| 别名 | 芴 | ||
| Canonical SMILES | C1(C=CC=C2)=C2CC3=C1C=CC=C3 | ||
| 分子式 | C13H10 | 分子量 | 166.22 |
| 溶解度 | DMSO: 62.5 mg/mL (376.01 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.0161 mL | 30.0806 mL | 60.1612 mL |
| 5 mM | 1.2032 mL | 6.0161 mL | 12.0322 mL |
| 10 mM | 0.6016 mL | 3.0081 mL | 6.0161 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Fluorene/fluorenone carboxamide derivatives as selective light-up fluorophores for c-myc G-quadruplex
Bioorg Med Chem Lett 2021 Mar 15;36:127824.PMID:33513388DOI:10.1016/j.bmcl.2021.127824.
The development of fluorescent dyes capable of selective recognition of G-quadruplexes is essential for studying its localization and biological functions. However, considering the G-quadruplex topologies may vary significantly, the synthesis of compounds showing both selectivity and strong fluorescence properties still remains a great challenge. Recently we have developed Fluorene/fluorenone derivatives with structure-specific binding towards dsRNA, indicating its potential for structure-selective ligands. Herein, we report the synthesis of novel Fluorene/fluorenone derivatives and their selectivity towards various DNA structures, particularly G-quadruplexes, two of which showed strong affinity to the proto-oncogene c-myc promoter G-quadruplex.
Synergistic degradation of Fluorene in soil by dielectric barrier discharge plasma combined with P25/NH2-MIL-125(Ti)
Chemosphere 2022 Jun;296:133950.PMID:35176305DOI:10.1016/j.chemosphere.2022.133950.
Plasma techniques to degrade pollutants are generally more efficient than conventional methods, but exist some problems such as high energy consumption, incomplete degradation of pollutants, and secondary pollution caused by highly toxic intermediates. In this study, the dielectric barrier discharge plasma (DBDP) combined with the Ti-based metal organic frameworks (MOFs) catalysts (P25/NH2-MIL-125(Ti)) was used to degrade Fluorene in the soil. The synergistic treatment technique used in soil remediation can realize a green and promising treatment efficiency with relatively low energy consumption. Compared with DBDP system alone, the synergetic treatment system of DBDP and P25/NH2-MIL-125(Ti) considerably increased the degradation efficiency of Fluorene in the soil to above 90% at 10 min, even with a relatively low discharge voltage (5 kV). The synergistic treatment system achieved 88.8% of Fluorene mineralization at 60 min. Optical emission spectroscopy and electron paramagnetic resonance spectroscopy both showed that •OH and •O2- played an important role in the synergetic treatment system. Nine main intermediates were identified using gas chromatography-mass spectrometry and Fourier transform infrared analysis. The main degradation of fluorine in soil was caused by the electronic transition of the catalytic material excited by DBDP, and finally mineralized into CO2 and H2O. The Fluorene and its toxic intermediates were effectively removed. This study provides an insight for achieving high efficiency and environmentally friendly application perspective in soil remediation.
Vinyl-Fluorene Molecular Wires for Voltage Imaging with Enhanced Sensitivity and Reduced Phototoxicity
J Am Chem Soc 2021 Aug 11;143(31):11903-11907.PMID:34323478DOI:10.1021/jacs.1c04543.
Fluorescent voltage indicators are an attractive alternative for studying the electrical activity of excitable cells; however, the development of indicators that are both highly sensitive and low in toxicity over long-term experiments remains a challenge. Previously, we reported a fluorene-based voltage-sensitive fluorophore that exhibits much lower phototoxicity than previous voltage indicators in cardiomyocyte monolayers, but suffers from low sensitivity to membrane potential changes. Here, we report that the addition of a single vinyl spacer in the Fluorene molecular wire scaffold improves the voltage sensitivity 1.5- to 3.5-fold over fluorene-based voltage probes. Furthermore, we demonstrate the improved ability of the new vinyl-fluorene VoltageFluors to monitor action potential kinetics in both mammalian neurons and human-induced pluripotent stem cell-derived cardiomyocytes. Addition of the vinyl spacer between the aniline donor and Fluorene monomer results in indicators that are significantly less phototoxic in cardiomyocyte monolayers. These results demonstrate how structural modification to the voltage sensing domain have a large effect on improving the overall properties of molecular wire-based voltage indicators.
Evaluation of fluorene-caused ecotoxicological responses and the mechanism underlying its toxicity in Eisenia fetida: Multi-level analysis of biological organization
J Hazard Mater 2022 Sep 5;437:129342.PMID:35716570DOI:10.1016/j.jhazmat.2022.129342.
Fluorene is an important toxic chemical that exists ubiquitously in the environment, and it has also been suggested to exert potential deleterious effects on soil invertebrates. However, knowledge about the toxic effects of Fluorene and its underlying mechanisms of the effects on key soil organism earthworms remains limited. From this view point, this study was undertaken to explore the potential effects of Fluorene and its underlying mechanisms in Eisenia fetida at the level of experimental animals, tissue, cell, and molecule. It was concluded that Fluorene exerted lethal activity to adult E. fetida on day 14 with the LC50 determined to be 88.61 mg/kg. Fluorene-induced ROS caused oxidative stress in E. fetida, resulting in DNA damage, protein carbonylation, and lipid peroxidation. Moreover, changed antioxidative enzymatic activities, non-enzymatic antioxidative activities, and total antioxidative capacity in E. fetida by Fluorene stress are associated with antioxidative and protective effects. High-dose Fluorene (> 2.5 mg/kg) exposure significantly caused histopathological lesions including the microstructure of body wall, intestine, and seminal vesicle of earthworms. Also, the reproductive system of E. fetida was clearly disrupted by Fluorene stress, leading to poor reproduction ability (decreased cocoon and juvenile production) in earthworms. It is found that E. fetida growth was significantly inhibited when treated with high-dose Fluorene, thereby causing normal growth disorders. Additionally, Fluorene stress triggered the abnormal mRNA expression related to oxidative stress (e.g., metallothionein and heat shock protein 70), growth (translationally controlled tumour protein), reproduction (annetocin precursor) in E. fetida. Together, both high-dose and long-term exposure elicited more severe poisoning effects on earthworms using the Integrated Biological Response (IBR) index, and E. fetida coelomocyte DNA was the most negatively affected by Fluorene stress. This study comprehensively evaluated fluorene-induced toxicity in E. fetida, and its underlying molecular mechanisms mediating the toxic responses have been elucidated. These findings provide valuable data for assessing potential ecological risks posed by fluorene-contaminated soil.
Bis-periazulene (Cyclohepta[ def]Fluorene) as a Nonalternant Isomer of Pyrene: Synthesis and Characterization of Its Triaryl Derivatives
J Am Chem Soc 2022 Mar 2;144(8):3370-3375.PMID:35188785DOI:10.1021/jacs.2c00476.
Bis-periazulene (cyclohepta[def]Fluorene), which is an unknown pyrene isomer, was synthesized as kinetically protected forms. Its triaryl derivatives 1c-e exhibited the superimposed electronic structures of peripheral, polarized, and open-shell π-conjugated systems. In contrast to previous theoretical predictions, bis-periazulene derivatives were in the singlet ground state. Changing an aryl group controlled the energy gap between the lowest singlet-triplet states.