Fluorescein-12-dUTP
(Synonyms: Fluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt) 目录号 : GC12016
Incorporated into DNA/cDNA by PCR, Nick Translation or other application for dye-labeled probe producing, used in FISH, microarray and other aspects
Cas No.:214154-36-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP. The Dye-labeled DNA/cDNA probes acquired are ideally suited for fluorescence hybridization applications - FISH or microarray-based gene expression profiling. Optimal substrate properties and labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommendatory fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/ 70-50% dTTP. Please note: Please protect the Dye-labeled dUTP from exposure to light and experiment under low light conditions. The optimal final concentration of the Dye-labeled dUTP might very depending on the practical application and assay conditions. For generating optimal product yields and high incorporation rates, an individual optimization for the Dye-labeled-dUTP/dTTP ratio is recommended.
Fluorescein-12-dUTP is used as a substrate for:
- Taq polymerase
- phi29 DNA Polymerase
- DNA Polymerase I (holoenzyme and Klenow fragment)
- Terminal Transferase
- Reverse transcriptase (e.g., from AMV and M-MuLV).
References:
[1]. Wiegant et al. (2001) Probe Labeling and Fluorescence in situ Hybridization. Current Protocols in Cytometry 8:8.3.1.
[2]. Yie SM1, Xiao R, et, al. Progesterone regulates HLA-G gene expression through a novel progesterone response element. Hum Reprod. 2006 Oct;21(10):2538-44. Epub 2006 May 9.
| Cas No. | 214154-36-6 | SDF | |
| 别名 | Fluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt | ||
| 化学名 | (Z)-3',6'-dihydroxy-N-((Z)-6-hydroxy-6-(((E)-3-(4-hydroxy-1-((2R,4S,5R)-4-hydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2-oxo-1,2-dihydropyrimidin-5-yl)allyl)imino)hexyl)-3-oxo-3H-spiro[isobenzofuran | ||
| Canonical SMILES | OC1=CC(O2)=C(C3(C4=C2C=C(O)C=C4)C5=C(C(O3)=O)C=C(/C(O)=N/CCCCC/C(O)=N/C/C([H])=C([H])/C(C(O)=NC6=O)=CN6[C@@]7([H])C[C@@](O)([H])[C@@](O7)([H])COP(O)(OP(O)(OP(O)(O)=O)=O)=O)C=C5)C=C1 | ||
| 分子式 | C39H41N4O21P3 (free acid) | 分子量 | 994.69 g/mol (free acid) |
| 溶解度 | Soluble in sterile water，pH 7.5 ±0.5 | 储存条件 | store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0053 mL | 5.0267 mL | 10.0534 mL |
| 5 mM | 0.2011 mL | 1.0053 mL | 2.0107 mL |
| 10 mM | 0.1005 mL | 0.5027 mL | 1.0053 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。