Fluorescein-12-UTP, 10mM Sodium Solution

Fluorescein-12-UTP is a fluorescein-labeled uridine triphosphate (UTP) analog that can be used as a substrate catalyzed by T3/T7/SP6 RNA polymerase and reverse transcriptase to replace UTP in in vitro transcription reactions for RNA mark[1]. Labeled RNA can be detected directly by fluorescence or indirectly by using an anti-luciferin horseradish peroxidase (HRP) or alkaline phosphatase (Alkaline Phosphatase, AP) conjugated antibody [2].

References:
[1]. Gilbert Lauter, Iris SÖll, Giselbert Hauptmann. Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain. 2011 Apr 5;6:10. doi: 10.1186/1749-8104-6-10.
[2]. Brent M. Bany, David G. Simmons. Nonradioactive In Situ Hybridization: Optimization for Tissue Sections from Pregnant Uteri and Placenta during the First Half of Pregnancy. 2014, Pages 591-603. https://doi.org/10.1016/B978-0-12-394445-0.00050-3

Fluorescein-12-UTP是一种荧光素标记的UTP类似物,可作为T3/T7/SP6 RNA 聚合酶和逆转录酶催化的底物在体外转录反应中替代UTP进行RNA标记[1]。被标记的RNA能够直接通过荧光检测,也可以通过与抗荧光素辣根过氧化物酶(Horseradish Peroxidase, HRP)或碱性磷酸酶(Alkaline Phosphatase, AP)偶联抗体联合使用进行间接检测[2]。