Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide)
(Synonyms: 荧光素-5-马来酰亚胺; N-(5-Fluoresceinyl)maleimide) 目录号 : GC30023
A label for sulfhydryl-containing proteins
Cas No.:75350-46-8
Sample solution is provided at 25 µL, 10mM.
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Fluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins.1,2,3 The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond.4 The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.5
1.Curtis, S.K., and Cowden, R.R.Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedureHistochemistry68(1)23-28(1980) 2.Elliott, J.T., Tona, A., and Plant, A.L.Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopyCytometry52(2)90-100(2003) 3.Lepedda, A.j., Zinellu, A., Nieddu, G., et al.Protein sulfhydryl group oxidation and mixed-disulfide modifications in stable and unstable human carotid plaquesOxid. Med. Cell. Longev.403973(2013) 4.Bigelow, D.J., and Inesi, G.Frequency-domain fluorescence spectroscopy resolves the location of maleimide-directed spectroscopic probes within the tertiary structure of the Ca-ATPase of sarcoplasmic reticulumBiochemistry30(8)2113-2125(1991) 5.Taraska, J.W., and Zagotta, W.N.Fluorescence applications in molecular neurobiologyNeuron66(2)170-189(2010)
| Cas No. | 75350-46-8 | SDF | |
| 别名 | 荧光素-5-马来酰亚胺; N-(5-Fluoresceinyl)maleimide | ||
| Canonical SMILES | O=C(C=C1)N(C2=CC3=C(C4(C5=C(OC6=C4C=CC(O)=C6)C=C(O)C=C5)OC3=O)C=C2)C1=O | ||
| 分子式 | C24H13NO7 | 分子量 | 427.36 |
| 溶解度 | DMSO : ≥ 32 mg/mL (74.88 mM) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3399 mL | 11.6997 mL | 23.3995 mL |
| 5 mM | 0.468 mL | 2.3399 mL | 4.6799 mL |
| 10 mM | 0.234 mL | 1.17 mL | 2.3399 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet