Fluorescein Biotin
(Synonyms: FITC Biotin) 目录号 : GC43685
A fluorescent biotin derivative
Cas No.:134759-22-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fluorescein biotin (FITC biotin) is a fluorescent biotin derivative and avidin and streptavidin ligand that contains a FITC fluorophore which displays excitation/emission maxima of 485/535 nm, respectively. FITC fluorescence is quenched upon binding to avidin or streptavidin and decreases in fluorescence intensity have been used to quantify biotin binding stoichiometry.
| Cas No. | 134759-22-1 | SDF | |
| 别名 | FITC Biotin | ||
| Canonical SMILES | S=C(NCCCCCNC(CCCCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O)NC3=CC=C4C(C(OC45C(C=CC(O)=C6)=C6OC7=C5C=CC(O)=C7)=O)=C3 | ||
| 分子式 | C42H50N6O8S2 | 分子量 | 831 |
| 溶解度 | DMF: Soluble,DMSO: Soluble,Methanol: Soluble,Water: Slightly Soluble | 储存条件 | Store at -20°C,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2034 mL | 6.0168 mL | 12.0337 mL |
| 5 mM | 0.2407 mL | 1.2034 mL | 2.4067 mL |
| 10 mM | 0.1203 mL | 0.6017 mL | 1.2034 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Dynamics of fluorescence dequenching of ostrich-quenched Fluorescein Biotin: a multifunctional quantitative assay for biotin
Anal Biochem 2005 Jul 15;342(2):221-8.PMID:15913533DOI:10.1016/j.ab.2005.03.046.
We describe a simple and rapid quantitative assay for biotin and biotin conjugates. The assay is based on the kinetic analysis of the enhancement of fluorescence of streptavidin/Fluorescein Biotin complexes in the presence of biotin. The kinetic response of fluorescence enhancement is proportional to the concentration of biotin. Standard calibration curves based on the kinetic response are obtained and detection limits of approximately 10(-9)M are established. Because the assay is amenable for use in small volumes of 5-50 microL or bead-based assays, the detection limits can be extended to the femtomole range. Since the assay depends on kinetic analysis, routine quantitation can be achieved without reference to standard curves. The dynamic aspects allow the assay to be extended to a broader range of applications including its use as an indicator of reagent mixing in laminar-flow assays carried out in microfluidic devices.