Fluorescein di(β-D-galactopyranoside) (FDG)
(Synonyms: 荧光素二(BETA-D-吡喃半乳糖苷),FDG) 目录号 : GC33589
A fluorescent substrate for β-galactosidase
Cas No.:17817-20-8
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Fluorescein di-β-galactopyranoside is a fluorogenic substrate for β-galactosidase.1 Upon enzymatic cleavage by β-galactosidase, fluorescein is released and its fluorescence can be used to quantify β-galactosidase activity. Fluorescein displays excitation/emission maxima of 490/514 nm, respectively.2,1
1.Plovins, A., Alvarez, A.M., Iba?ez, M., et al.Use of fluorescein-di-β-D-galactopyranoside (FDG) and C12-FDG as substrates for β-galactosidase detection by flow cytometry in animal, bacterial, and yeast cellsAppl. Environ. Microbiol.60(12)4638-4641(1994) 2.Hermanson, G.T.Fluorescent ProbesBioconjugate Techniques(2013)
|
Cell experiment: |
The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1]. |
|
References: [1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43. |
|
| Cas No. | 17817-20-8 | SDF | |
| 别名 | 荧光素二(BETA-D-吡喃半乳糖苷),FDG | ||
| Canonical SMILES | O=C1OC2(C(C=CC(O[C@@H]([C@@H]([C@@H](O)[C@H]3O)O)O[C@@H]3CO)=C4)=C4OC5=CC(O[C@@H]([C@@H]([C@@H](O)[C@H]6O)O)O[C@@H]6CO)=CC=C25)C7=C1C=CC=C7 | ||
| 分子式 | C32H32O15 | 分子量 | 656.59 |
| 溶解度 | DMSO : 5.56 mg/mL (8.47 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C, protect from light,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.523 mL | 7.6151 mL | 15.2302 mL |
| 5 mM | 0.3046 mL | 1.523 mL | 3.046 mL |
| 10 mM | 0.1523 mL | 0.7615 mL | 1.523 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet