Flusilazole
(Synonyms: 氟硅唑; DPX-H6573) 目录号 : GC63668
Flusilazole (DPX-H6573) 是一种有机硅烷杀菌剂 (fungicide)，具有广谱抗真菌作用。
Cas No.:85509-19-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Flusilazole (DPX-H6573), an organosilane fungicide, has broad-spectrum antifungal effect. Flusilazole exhibits curative and preventative activities and is recommended for use in agriculture and horticulture[1].
[1]. D Fabio Mercado, et al. Reaction Kinetics and Mechanisms of Organosilicon Fungicide Flusilazole With Sulfate and Hydroxyl Radicals. Chemosphere. 2018 Jan;190:327-336.
| Cas No. | 85509-19-9 | SDF | |
| 别名 | 氟硅唑; DPX-H6573 | ||
| 分子式 | C16H15F2N3Si | 分子量 | 315.39 |
| 溶解度 | DMSO : 100 mg/mL (317.07 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1707 mL | 15.8534 mL | 31.7068 mL |
| 5 mM | 0.6341 mL | 3.1707 mL | 6.3414 mL |
| 10 mM | 0.3171 mL | 1.5853 mL | 3.1707 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Flusilazole-induced damage to SerW3 cells via cytotoxicity, oxidative stress and lipid metabolism: An in vitro study
Pestic Biochem Physiol 2022 Jan;180:104998.PMID:34955182DOI:10.1016/j.pestbp.2021.104998.
Flusilazole (C16H15F2N3Si) is a triazole fungicide and it is being used widely in recent years to control fungal infections in various fruits and vegetables. This study aims to evaluate the impact of Flusilazole on cytotoxicity, ATP-dependent cassette transporter proteins (ABC transporter proteins) in SerW3 cells. In this study, SerW3 cells have administrated with 25, 100, and 200 μM Flusilazole, cell viability was performed. The quantity of the cellular lipids was evaluated spectrophotometrically. Moreover, the expression of the ABCA1 and ABCB1 proteins determined by immunofluorescence microscopy. Furtherly, evaluation of the cell death type and measurement of the activity of the antioxidant enzymes was performed. According to the results, Flusilazole treatment gave rise to inhibition in cell viability, increase in apoptotic cell number, reduction in cellular lipids, and inhibition in the expression of ABCA1 and ABCB1 proteins. Furthermore, it caused decreases in antioxidant enzyme activities. It may be concluded that Flusilazole administration may cause infertility/subfertility. The mechanism of action can be due to cytotoxicity, impairment of the detoxification mechanisms, lipid metabolism, and dysregulation of cell functions.
Hormetic Effects of Flusilazole Preconditioning on Mycelial Growth and Virulence of Sclerotinia sclerotiorum
Plant Dis 2018 Jun;102(6):1165-1170.PMID:30673443DOI:10.1094/PDIS-10-17-1638-RE.
Hormetic effects of fungicides are highly relevant to fungicide applications and management of plant-pathogenic fungi. Preconditioning (i.e., early exposure to relatively low doses of a toxicant) is a special form of hormesis, and fungicide preconditioning of phytopathogenic fungi is inevitable in the field. The present study showed that spraying the demethylation inhibitor (DMI) fungicide Flusilazole at 0.1 µg/ml had stimulatory effects on the virulence of Sclerotinia sclerotiorum inoculated at 1 and 24 h after spraying. Flusilazole sprayed at 10 µg/ml showed inhibitory effects on the virulence of S. sclerotiorum inoculated during the first 3 days after spraying. Inoculations on the 5th, 7th, and 10th day after spraying did not show any significant inhibitory or stimulatory effects on the virulence. After growing for 2 days on potato dextrose agar (PDA) amended with Flusilazole at a dose range from 0.0005 to 0.25 µg/ml as preconditioning treatments, mycelia were transferred onto PDA without fungicide and subsequent mycelial growth was slower than the nonpreconditioned control. However, after the preconditioned colonies were transferred onto PDA supplemented with Flusilazole at 0.2 µg/ml, percent stimulations of mycelia growth compared with the control had a parabolic shape across the preconditioning Flusilazole concentration range. Similarly, the mycelial growth of the preconditioned mycelial plugs on PDA amended with other DMI fungicides (prochloraz or tebuconazole) also showed a typical hormetic response, whereas mycelial growth on PDA amended with carbendazim or dimethachlone was inhibited in a dose-dependent manner. Preconditioning S. sclerotiorum with Flusilazole on rapeseed plants elicited virulence stimulations in a dose-dependent manner similar to those on mycelial growth on PDA. After disease lesions developed on rapeseed leaves sprayed with Flusilazole as the preconditioning treatment were inoculated onto rapeseed plants, virulence was inhibited on leaves without fungicide or sprayed with carbendazim or dimethachlone compared with the nonpreconditioned control, whereas virulence was stimulated on leaves sprayed with Flusilazole, prochloraz, or tebuconazole, and the maximum percent stimulation was 10.2%. These results will advance our understanding of hormetic effects of fungicides and of preconditioning hormesis in particular.
Flusilazole Induced Cytotoxicity and Inhibition of Neuronal Growth in Differentiated SH-SY5Y Neuroblastoma Cells by All-Trans-Retinoic Acid (Atra)
Turk J Pharm Sci 2021 Oct 28;18(5):597-603.PMID:34719187DOI:10.4274/tjps.galenos.2021.30676.
Objectives: Flusilazole (FLUS) is a broad-spectrum organosilicon triazole fungicide used for protecting economically important cereals and orchard fruits. Considering the exposure route of pesticides, pesticide contamination of food is inevitable. Furthermore, excessive exposure to pesticides causes health problems in both target and non-target organisms. It was aimed to evaluate the effects of the triazole fungicide FLUS on cytotoxicity and neurite extension in differentiated SH-SY5Y neuroblastoma cells. Materials and methods: The SH-SY5Y cells were differentiated into mature neurons using 10-µM all-trans-retinoic acid (RA) treatment for 7 days. Then the differentiated SH-SY5Y cells were treated with 50, 100 and 200 μM FLUS for 24 h. Afterwards, cell viability assays were performed including crystal violet, neutral red cell viability, and lactate dehydrogenase leakage assays. The morphological examinations were performed and neurite lenghts of the cells were measured in all experimental groups. Results: FLUS treatment induced cytotoxicity in SH-SY5Y cells differentiated with RA. Significant decreases in cell viability percentages were observed. Furthermore, neurite lengths were negatively affected by the treatment of FLUS at the highest concentration. Conclusion: FLUS is a fungicide widely used in agriculture to protect crops from fungal diseases. However, the intensive use of these compounds causes a potential risk to human and environmental health. According to the results of the study, it can be concluded that high concentrations of FLUS cause neurotoxicity by causing neural cell death and adverse effects on neurite outgrowth in differentiated SH-SY5Y cells. FLUS exposure can cause neuronal degeneration in mammals.
Rapid Detection of Flusilazole in Pears with Au@Ag Nanoparticles for Surface-Enhanced Raman Scattering
Nanomaterials (Basel) 2018 Feb 8;8(2):94.PMID:29419755DOI:10.3390/nano8020094.
Residual pesticides in vegetables or fruits have been become one of the world's most concerned food safety issues. Au-Ag core-shell nanoparticles (Au@Ag NPs) coupled with surface-enhanced Raman spectroscopy (SERS) was used for analysis of Flusilazole which was widely applied in pears. Three different diameters of Au@Ag NPs were prepared to select the best SERS substrate for analyzing Flusilazole. The Au@Ag NPs sizes of 90 ± 7 nm showed the highest enhancement effect and could be detected Flusilazole standard solution and the minimum detectable concentration was 0.1 mg/L. Flusilazole in pear could also identified at as low as 0.1 μg/g. The amount of adsorbent is critical in the sample preparation process and the best amount of each absorber dosage was 0.6 g MgSO₄, 0.2 g C18 and 0.2 g primary secondary amine (PSA). The experimental results indicated a good linear relationship between the Raman intensities of chief peaks and the concentrations of Flusilazole solutions (R² = 0.924-0.962). This study shows that Au@Ag as SERS substrate has great potential to analyze of Flusilazole in food matrices.
Stimulatory Effects of Flusilazole on Virulence of Sclerotinia sclerotiorum
Plant Dis 2018 Jan;102(1):197-201.PMID:30673466DOI:10.1094/PDIS-07-17-1041-RE.
Flusilazole, a member of the demethylation inhibitor fungicides, is highly efficacious for control of Sclerotinia sclerotiorum. To achieve judicious applications of Flusilazole, its hormetic effects on virulence of S. sclerotiorum were investigated. Flusilazole sprayed at concentrations from 0.02 to 0.5 μg/ml caused statistically significant (P < 0.05) stimulatory effects on virulence of S. sclerotiorum to potted rapeseed plants, and the maximum stimulation magnitudes were 11.0 and 10.7% for isolates GS-7 and HN-24, respectively. Studies on the time course of the infection process showed that a stimulatory effect on virulence could be discerned at 18 h postinoculation, indicating a direct stimulation mechanism rather than an overcompensation for initial inhibitions. In order to determine whether the stimulations were caused mainly by effects of Flusilazole on S. sclerotiorum or on rapeseed plants, mycelia grown on flusilazole-amended potato dextrose agar (PDA) media were inoculated on leaves of rapeseed plants without spraying the fungicide. Mycelium radial growth on PDA supplemented with Flusilazole at concentrations from 0.005 to 0.16 μg/ml was inhibited by 10.11 to 48.7% for isolate GS-7 and by 4.1 to 24.9% for isolate HN-24. Observations with a scanning electron microscope showed that Flusilazole in PDA at 0.04 and 0.08 μg/ml caused slightly deformed mycelia and twisted mycelial tips. Nevertheless, after inoculating on leaves of potted rapeseed plants, virulence of the inhibited mycelia was statistically significantly (P < 0.05) greater than that of the nontreated control, and the maximum stimulation magnitudes were 16.2 and 19.8% for isolates GS-7 and HN-24, respectively. Studies on a physiological mechanism for virulence stimulations showed that tolerance to hydrogen peroxide did not increase significantly for mycelia grown on flusilazole-amended PDA, thus excluding the possibility of tolerance to reactive oxygen species as a potential mechanism for virulence stimulations.