Fructose
(Synonyms: 果糖) 目录号 : GN10057
果糖是在许多植物中发现的一种简单的酮单糖，它通常与葡萄糖结合形成二糖蔗糖。
Cas No.:7660-25-5
Sample solution is provided at 25 µL, 10mM.
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Fructose is a simple ketonic monosaccharide found in many plants, where it is often bonded to glucose to form the disaccharide sucrose.
Fructose, at low concentrations do not cause any significant increase of Tissue factor (TF)-mRNA levels. On the contrary, higher Fructose concentrations cause increase in TF mRNA levels at 60 min, as compare to unstimulated cells. Increasing Fructose concentrations causes significant decrease of tPA-mRNA levels. SOD significantly prevents Fructose induced NF-κB activation which is associated with the parallel reduction of Fructose-induced TF expression/activity[1].
In mice fed 0% Fructose, portal (0.060±0.006 mM, overall mean for all time points) and systemic (0.030±0.003 mM) Fructose concentrations do not vary with time after feeding. In contrast, portal concentrations in wild-type mice consuming 20% Fructose increase by more than twofold from time (t)=0 to t=1 h after feeding (~0.13 mM). Likewise, systemic serum Fructose goes from 0.037 at t=0 to 0.13 mM 1 h after feeding. Fasted (t=0) serum Fructose in the 20% group is similar to postprandial concentrations in the 0% mice for both portal and systemic levels, suggesting that the baseline Fructose concentration during fasting is not affected by diet. Serum Fructose concentrations in KHK-/- mice are 5- to 100-fold greater than those in wild-type mice for the same diet, time, and sample location. Mean (for all time points) portal and systemic glucose concentrations in mice fed 20% Fructose are ~3 (P=0.004) and ~2 (P=0.04) mM greater, respectively, than those in mice fed 0%. Systemic Fructose concentrations are approximately threefold greater in KHK-/- mice fed Fructose compare with those fed glucose, but are similar between glucose- and Fructose-fed wild-type mice[2].
References:
[1]. Cirillo P, et al. Fructose induces prothrombotic phenotype in human endothelial cells : A new role for "added sugar" in cardio-metabolic risk. J Thromb Thrombolysis. 2015 Nov;40(4):444-51.
[2]. Patel C, et al. Effect of dietary fructose on portal and systemic serum fructose levels in rats and in KHK-/- and GLUT5-/- mice. Am J Physiol Gastrointest Liver Physiol. 2015 Nov 1;309(9):G779-90.
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Cell experiment: |
HUVECs are incubated with Fructose (0.25, 1 and 2.5 mM) for 30 min. Then, cells are washed with PBS and then fresh medium is added. Total mRNA is extracted by cell cultures using TRIzol reagent, at baseline and 60 min after Fructose stimulation and Tissue factor (TF) mRNA levels are examined by realtime reverse transcription (RT) and polymerase chain reaction (PCR). In positive control experiments, HUVECs are incubated with LPS (50 μg/mL), for 30 min and then mRNA is extracted at 60 min[1]. |
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Animal experiment: |
50 young adult (7-wk-old) male C57BL6 wild-type mice (~18 g) are divided into 10 cages and acclimatized to a reversed light cycle. Mice are fed a nonpurified commercial diet ad libitum for the first 4 days. On the 5th day and then throughout the experiment, diets are removed at 2001 (lights on) and returned at 0801 (lights off). For days 8 to 14, diets are switched to pellets containing either 0% Fructose, 10% sucrose, 20% glucose (termed as "0% Fructose") or 20% Fructose, 10% sucrose, or 0% glucose (20% Fructose). On the 15th day, mice are killed at 0800 before feeding and 0900, 1030, 1200, and 1530 during the dark phase, with n=5 for each time point and diet[2]. |
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References: [1]. Cirillo P, et al. Fructose induces prothrombotic phenotype in human endothelial cells : A new role for "added sugar" in cardio-metabolic risk. J Thromb Thrombolysis. 2015 Nov;40(4):444-51. |
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| Cas No. | 7660-25-5 | SDF | |
| 别名 | 果糖 | ||
| 化学名 | (2R,3S,4R,5R)-2-(hydroxymethyl)oxane-2,3,4,5-tetrol | ||
| Canonical SMILES | C1C(C(C(C(O1)(CO)O)O)O)O | ||
| 分子式 | C6H6O6 | 分子量 | 180.06 |
| 溶解度 | ≥ 18mg/mL in Water | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.5537 mL | 27.7685 mL | 55.537 mL |
| 5 mM | 1.1107 mL | 5.5537 mL | 11.1074 mL |
| 10 mM | 0.5554 mL | 2.7769 mL | 5.5537 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet