G CSF Human
目录号 : GP20318Granulocyte-Colony Stimulating Factor Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | CSF-3; MGI-1G; GM-CSF beta; Pluripoietin; Filgrastim; Lenograstim; G-CSF; MGC45931; GCSF. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids of GCSF was determined and was found to be Met-Thr-Pro-Leu-Gly. | ||
| Solubility | It is recommended to reconstitute the lyophilized GCSF in sterile20mM AcOHnot less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 100,000,000 IU/mg. | ||
| Formulation | GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4. | ||
GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSFgene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes.
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 100,000,000 IU/mg.
Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.