(Gly¹⁴)-Humanin (human)
目录号 : GA20196
Humanin 既是细胞内蛋白又是分泌蛋白。
Cas No.:330936-70-4
Sample solution is provided at 25 µL, 10mM.
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Humanin is both an intracellular protein and a secreted protein. It is the first newly discovered peptide encoded in the mitochondrial genome, which exerts robust protective effects against a myriad of cytotoxic stimuli in many cell types[2]. Replacement of serine at position 14 of the HN peptide chain (Ser14) with glycine (Gly) leads to the formation of (Gly14)-Humanin (human), which has cytoprotective activity enhanced by 1000-fold when compared with that of Humanin[3].
(Gly14)-Humanin (human) reduced Dil-ox-LDL accumulation in the RAW 264.7 macrophages. (Gly14)-Humanin (human) could inhibit ox-LDL-induced macrophage-derived foam cell formation, which occurs because of a decrease in lipid uptake and an increase in cholesterol efflux from macrophage cells[7]. (Gly14)-Humanin (human) pretreatment inhibits HG-induced apoptosis of vascular endothelial cells by down-regulating miR-155, which was transferred to adjacent vascular endothelial cells via cell-derived EMPs. EMPs could serve as a potential tool for the specific delivery of miRNAs, contributing to a stronger therapeutic effect of (Gly14)-Humanin (human) in anti-diabetic vascular complications therapy[1]. (Gly14)-Humanin (human) could restore cathepsin D activity and protein level in HUVECs to repair lysosomal functional damage induced by Ox-LDL, further repairing Ox-LDL-induced autophagic damage in HUVECs[4].
Pretreatment with (Gly14)-Humanin (human) not only protected spatial memory but also rescued STAT3 from Aβ-induced disruption; and the neuroprotective effects of (Gly14)-Humanin (human) were effectively counteracted by genistein, a specific tyrosine kinase inhibitor. Sequence 31-35 in Aβ is the shortest active center responsible for the neurotoxicity of Aβ from molecule to behavior; and (Gly14)-Humanin (human) protects spatial learning and memory in rats against Aβ-induced insults; and probably involves the activation of tyrosine kinases and subsequent beneficial modulation of STAT3 and caspase3[5]. Mice administered (Gly14)-Humanin (human) intracerebroventricularly (i.c.v.) prior to TBI had decreased cells with plasmalemma permeability in the injured cortex and hippocampus, reduced brain lesion volume, improved motor performance and ameliorated performance in the Morris water maze test post TBI. Reduced lesion volume was also observed even when (Gly14)-Humanin (human) was administered intraperitoneally (i.p.) at 1h and 2h post TBI, and minor amelioration in motor and Morris water maze test deficits was also observed. (Gly14)-Humanin (human) treatment improved morphological and functional outcomes after TBI in mice and the protective effect of (Gly14)-Humanin (human) against TBI may be associated with down-regulating apoptosis and autophagy[6].
References:
[1]: Shen MY, Wang M, et,al. [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles. Diabetes Metab Syndr Obes. 2021 May 24;14:2335-2347. doi: 10.2147/DMSO.S306026. PMID: 34079312; PMCID: PMC8163639.
[2]: Kim SJ, Mehta HH, et,al. Mitochondrial peptides modulate mitochondrial function during cellular senescence. Aging (Albany NY). 2018 Jun 10;10(6):1239-1256. doi: 10.18632/aging.101463. PMID: 29886458; PMCID: PMC6046248.
[3]: Hashimoto Y, Niikura T, et,al. A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Abeta. Proc Natl Acad Sci U S A. 2001 May 22;98(11):6336-41. doi: 10.1073/pnas.101133498. Erratum in: Proc Natl Acad Sci U S A 2001 Oct 23;98(22):12854. PMID: 11371646; PMCID: PMC33469.
[4]: Ding Y, Feng Y, et,al. [Gly14]-humanin restores cathepsin D function via FPRL1 and promotes autophagic degradation of Ox-LDL in HUVECs. Nutr Metab Cardiovasc Dis. 2020 Nov 27;30(12):2406-2416. doi: 10.1016/j.numecd.2020.07.022. Epub 2020 Jul 25. PMID: 32917500.
[5]: Yuan L, Liu XJ, et,al. [Gly14]-Humanin Protects Against Amyloid β Peptide-Induced Impairment of Spatial Learning and Memory in Rats. Neurosci Bull. 2016 Aug;32(4):374-82. doi: 10.1007/s12264-016-0041-x. Epub 2016 Jun 15. PMID: 27306655; PMCID: PMC5563781.
[6]: Wang T, Zhang L, et,al. [Gly14]-Humanin reduces histopathology and improves functional outcome after traumatic brain injury in mice. Neuroscience. 2013 Feb 12;231:70-81. doi: 10.1016/j.neuroscience.2012.11.019. Epub 2012 Nov 20. PMID: 23178909.
[7]: Zhu WW, Wang SR, et,al. Gly[14]-humanin inhibits ox-LDL uptake and stimulates cholesterol efflux in macrophage-derived foam cells. Biochem Biophys Res Commun. 2017 Jan 1;482(1):93-99. doi: 10.1016/j.bbrc.2016.10.138. Epub 2016 Nov 1. PMID: 27815075.
Humanin 既是细胞内蛋白又是分泌蛋白。它是第一个新发现的在线粒体基因组中编码的肽，对许多细胞类型的无数细胞毒性刺激具有强大的保护作用[2]。 HN 肽链 14 位丝氨酸 (Ser14) 被甘氨酸 (Gly) 取代导致 (Gly14)-Humanin (human) 的形成，与 Humanin 相比，其细胞保护活性增强了 1000 倍[3].
(Gly14)-Humanin（人类）减少了 RAW 264.7 巨噬细胞中的 Dil-ox-LDL 积累。 (Gly14)-Humanin（人）可抑制 ox-LDL 诱导的巨噬细胞衍生泡沫细胞形成，这是由于巨噬细胞脂质摄取减少和胆固醇流出增加所致[7] . (Gly14)-Humanin（人）预处理通过下调 miR-155 抑制 HG 诱导的血管内皮细胞凋亡，miR-155 通过细胞衍生的 EMP 转移到邻近的血管内皮细胞。 EMP 可作为特异性递送 miRNA 的潜在工具，有助于增强 (Gly14)-Humanin (human) 在抗糖尿病血管并发症治疗中的疗效[1]。 (Gly14)-Humanin (human) 可恢复 HUVECs 组织蛋白酶 D 活性和蛋白水平，修复 Ox-LDL 诱导的溶酶体功能损伤，进一步修复 Ox-LDL 诱导的 HUVECs 自噬损伤[4] .
用 (Gly14)-Humanin（人）预处理不仅可以保护空间记忆，还可以从 Aβ 诱导的破坏中拯救 STAT3； (Gly14)-Humanin（人）的神经保护作用被染料木黄酮（一种特定的酪氨酸激酶抑制剂）有效抵消。 Aβ中的31-35序列是负责Aβ从分子到行为的神经毒性的最短活性中心； (Gly14)-Humanin（人类）保护大鼠的空间学习和记忆免受 Aβ 诱导的损伤；并且可能涉及酪氨酸激酶的激活和随后对 STAT3 和 caspase3 的有益调节[5]。在 TBI 前脑室内 (i.c.v.) 施用 (Gly14)-Humanin（人）的小鼠在受损皮层和海马体中具有质膜通透性的细胞减少、脑损伤体积减小、运动性能改善以及 TBI 后莫里斯水迷宫测试中的性能改善。即使在 TBI 后 1 小时和 2 小时腹膜内 (i.p.) 施用 (Gly14)-Humanin（人）时，也观察到损伤体积减小，并且还观察到运动和莫里斯水迷宫测试缺陷的轻微改善。 (Gly14)-Humanin（人）治疗改善了小鼠 TBI 后的形态学和功能结果，并且（Gly14）-Humanin（人）对 TBI 的保护作用可能与下调细胞凋亡和自噬有关[6]< /sup>.
| Cell experiment [1]: | |
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Cell lines |
EMP(from Primary HUVECs ) |
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Preparation Method |
For cells receiving (Gly14)-Humanin (human), cells were pretreated with (Gly14)-Humanin (human) for 3 hours. |
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Reaction Conditions |
1 µM (Gly14)-Humanin (human) for 3 hours |
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Applications |
(Gly14)-Humanin (human) can attenuate HG-induced apoptosis of HUVECs cells by reducing the expression of Mir-155 in EMPs. |
| Animal experiment [2]: | |
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Animal models |
10-12-week-old adult male CD-1 mice weighing 25-30 g |
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Preparation Method |
(Gly14)-Humanin (human) -treated groups were administered 0.1 µg of (Gly14)-Humanin (human) in 5 µL of saline intraventricularly (i.c.v., coordinates 1 mm posterior, 1 mm lateral, 2.5 mm deep to bregma) before TBI or 1 µg in 100 µL of saline intraperitoneally (i.p.) post TBI. |
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Dosage form |
0.1 µg of (Gly14)-Humanin (human) in 5 µL saline intraperitoneally post TBI. |
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Applications |
(Gly14)-Humanin (human) reduced TBI-induced cell damage. |
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References: [1]: Shen MY, Wang M, et,al. [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles. Diabetes Metab Syndr Obes. 2021 May 24;14:2335-2347. doi: 10.2147/DMSO.S306026. PMID: 34079312; PMCID: PMC8163639. | |
| Cas No. | 330936-70-4 | SDF | |
| 分子式 | C118H202N34O31S2 | 分子量 | 2657.25 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3763 mL | 1.8816 mL | 3.7633 mL |
| 5 mM | 0.0753 mL | 0.3763 mL | 0.7527 mL |
| 10 mM | 0.0376 mL | 0.1882 mL | 0.3763 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet