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Boc-Gly-Arg-Arg-AMC Sale

目录号 : GA21012

Boc-Gly-Arg-Arg-AMC是黄热病病毒NS3蛋白酶、登革热病毒NS2B-NS3蛋白酶以及西尼罗河病毒蛋白酶等黄病毒蛋白酶的底物。

Boc-Gly-Arg-Arg-AMC Chemical Structure

Cas No.:113866-14-1

规格 价格 库存 购买数量
25mg
¥6,670.00
现货
100mg
¥20,023.00
现货

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Sample solution is provided at 25 µL, 10mM.

Description

Boc-Gly-Arg-Arg-AMC is a substrate for flavivirus proteases such as West Nile virus protease, yellow fever virus NS3 protease, and dengue virus NS2B-NS3 protease. Boc-GRR-AMC is cleaved by type II Metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana. Stock solutions of Boc-GRR-AMC are best prepared in DMSO [1-2].

References:

[1]. Osman H, Idris NH, et,al. 3,5-Bis(arylidene)-4-piperidones as potential dengue protease inhibitors. Acta Pharm Sin B. 2017 Jul;7(4):479-484. doi: 10.1016/j.apsb.2017.04.009. Epub 2017 May 4. PMID: 28752033; PMCID: PMC5518655.
[2]. Abduraman MA, Hariono M, et,al. Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities. Heliyon. 2018 Dec 8;4(12):e01023. doi: 10.1016/j.heliyon.2018.e01023. PMID: 30560214; PMCID: PMC6289942.

Boc-Gly-Arg-Arg-AMC是黄热病病毒NS3蛋白酶、登革热病毒NS2B-NS3蛋白酶以及西尼罗河病毒蛋白酶等黄病毒蛋白酶的底物。Boc-GRR-AMC被 拟南芥II 型Metacaspases Atmc4和Atmc9切割。Boc-GRR-AMC的库存溶液最好在DMSO中制备[1-2]

实验参考方法

Protocol for protease assay [1]:

  1. Preparing an enzymatic reaction mixture with a total volume of 100µL containing standard buffer solution (Tris HCl buffer pH 8.5), a constant amount (0.57 µmol/L) of DENV2 NS2B-NS3 solution and the candidate inhibitor compound (200 mg/L in DMSO).
  2. The mixtures were incubated at 37℃ and shaken at 200 rpm for 10 min, and then 10 mM of peptide substrate Boc-Gly-Arg-Arg-AMC was added to initiate the reaction and incubation with shaking at 200 rpm was carried out for another 60 min.
  3. The assays were in triplicate and corrected by subtracting the absorbance of their respective blank.
  4. The selected compounds were used to generate an IC50 value in the range (6.25-200 mg/L) of sample concentration.
  5. The IC50 values were determined graphically from inhibition curves (log inhibitor concentration vs. percentage of inhibition) using graphpad module.

This protocol only provides a guideline, and should be modified according to your specific needs.

References:

[1]. Osman H, Idris NH, et,al. 3,5-Bis(arylidene)-4-piperidones as potential dengue protease inhibitors. Acta Pharm Sin B. 2017 Jul;7(4):479-484. doi: 10.1016/j.apsb.2017.04.009. Epub 2017 May 4. PMID: 28752033; PMCID: PMC5518655.

化学性质

Cas No. 113866-14-1 SDF
分子式 C29H44N10O7 分子量 644.73
溶解度 Soluble in DMSO 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 1.551 mL 7.7552 mL 15.5104 mL
5 mM 0.3102 mL 1.551 mL 3.1021 mL
10 mM 0.1551 mL 0.7755 mL 1.551 mL
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*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
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