CRAMP (mouse)
(Synonyms: 抗菌肽CRAMP) 目录号 : GA21286
CRAMP (mouse)是一种在胚胎和成年小鼠中表达的cathelin相关的抗菌肽（AMP）。
Cas No.:376364-36-2
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
CRAMP (mouse) is a cathelin-related antimicrobial peptide (AMP) expressed in embryonic and adult mice[1]. AMPs exert multiple immunomodulatory effects that may be dominant under physiological conditions and lose their bactericidal properties in serum and tissue environments[2]. CRAMP (mouse) is an effective antibiotic against Gram-negative bacteria by inhibiting the growth of multiple bacterial strains and directly permeabilizing the inner membrane of Escherichia coli[3]. Mice lacking CRAMP are more susceptible to central nervous system pneumococcal infection, resulting in higher bacterial loads within the brain and outside the central nervous system, accompanied by reduced neutrophil infiltration and higher mortality[4]. CRAMP (mouse) is able to enhance the chemotactic response of bone marrow-derived stem/progenitor cells (BMSPCs) to a low α-chemokine stromal-derived factor 1 (SDF-1) gradient[5].
References:
[1] Gallo R L, Kim K J, Bernfield M, et al. Identification of CRAMP, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse[J]. Journal of Biological Chemistry, 1997, 272(20): 13088-13093.
[2] Xia X, Cheng L, Zhang S, et al. The role of natural antimicrobial peptides during infection and chronic inflammation[J]. Antonie Van Leeuwenhoek, 2018, 111: 5-26.
[3] Mishra N M, Briers Y, Lamberigts C, et al. Evaluation of the antibacterial and antibiofilm activities of novel CRAMP–vancomycin conjugates with diverse linkers[J]. Organic & Biomolecular Chemistry, 2015, 13(27): 7477-7486.
[4] Kress E, Merres J, Albrecht L J, et al. CRAMP deficiency leads to a pro-inflammatory phenotype and impaired phagocytosis after exposure to bacterial meningitis pathogens[J]. Cell Communication and Signaling, 2017, 15: 1-15.
[5] Klyachkin Y M, Idris A, Rodell C B, et al. Cathelicidin related antimicrobial peptide (CRAMP) enhances bone marrow cell retention and attenuates cardiac dysfunction in a mouse model of myocardial infarction[J]. Stem cell reviews and reports, 2018, 14: 702-714.
CRAMP (mouse)是一种在胚胎和成年小鼠中表达的cathelin相关的抗菌肽（AMP）[1]。AMP发挥多种免疫调节作用，在生理条件下可能占主导地位，在血清和组织环境中会失去杀菌特性[2]。CRAMP (mouse)通过抑制多种细菌菌株的生长和直接透化大肠杆菌的内膜而成为针对革兰氏阴性菌的有效抗生素[3]。缺乏CRAMP的小鼠更容易受到中枢神经系统肺炎球菌感染，导致大脑内部和中枢神经系统外部的细菌负荷更高，并伴有中性粒细胞浸润减少和死亡率更高[4]。CRAMP (mouse)能够增强骨髓来源的干细胞/祖细胞（BMSPC）对低α-趋化因子基质衍生因子1（SDF-1）梯度的趋化反应[5]。
| Cas No. | 376364-36-2 | SDF | |
| 别名 | 抗菌肽CRAMP | ||
| 分子式 | C178H302N50O46 | 分子量 | 3878.67 |
| 溶解度 | DMSO : 3.33 mg/mL (0.86 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.2578 mL | 1.2891 mL | 2.5782 mL |
| 5 mM | 0.0516 mL | 0.2578 mL | 0.5156 mL |
| 10 mM | 0.0258 mL | 0.1289 mL | 0.2578 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet