H-Tyr-His-OH
目录号 : GA22973
H-Tyr-His-OH 保持为组氨酸定量分析的内标，并在 8.06 分钟时洗脱。
Cas No.:3788-44-1
Sample solution is provided at 25 µL, 10mM.
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H-Tyr-His-OH was maintained as an internal standard for histidine quantitative analysis and eluted at 8.06 min. In addition, since it does not exist in tissues as an endogenous peptide, it is easy to form positive ions through ESI ionization, with good recovery.[1,2]
References:
[1]: Aldini G, Orioli M,et,al. Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry. J Mass Spectrom. 2004 Dec;39(12):1417-28. doi: 10.1002/jms.696. PMID: 15578740.
[2]: Orioli M, Aldini G, et,al. LC-ESI-MS/MS determination of 4-hydroxy-trans-2-nonenal Michael adducts with cysteine and histidine-containing peptides as early markers of oxidative stress in excitable tissues. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 15;827(1):109-18. doi: 10.1016/j.jchromb.2005.04.025. PMID: 15916929.
H-Tyr-His-OH 作为组氨酸定量分析的内标，并在 8.06 分钟时洗脱。此外，由于它不作为内源性肽存在于组织中，通过ESI电离很容易形成正离子，回收率好。[1,2]
| Quantitative analysis experiment [1]: | |
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Samples Preparation |
Calibration samples were prepared by spiking lung homogenate (1 : 3, w/v, in PBS) with each working solution. |
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Preparation Method |
Samples (plasma or tissue homogenates from male rats) were prepared by protein precipitation with HClO(4) (1 : 1, v/v) containing H-Tyr-His-OH as internal standard. |
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Reaction Conditions |
Using 0.1% HFBA as ion-pairing agent: the histidine-containing dipeptides eluted at 4.54 min (CAR), 4.64 min (ANS, HCAR) and 5.06 min (BAL), with an excellent bell shape. H-Tyr-His-OH was chosen as IS for quantitative analysis. |
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Applications |
The mapping profile( H-Tyr-His-OH as internal standard) in rat tissue gave the following results: the highest concentrations of CAR and ANS were found in skeletal muscles (soleus, gastrocnemius, tibialis), followed by the heart, cerebellum and brain (ANS below the LOQ). HCAR was found only in the brain and cerebellum. No histidine-containing dipeptides were detectable in plasma, liver, kidney and lung. |
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References: [1]. Aldini G, Orioli M,et,al. Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry. J Mass Spectrom. 2004 Dec;39(12):1417-28. doi: 10.1002/jms.696. PMID: 15578740. |
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| Cas No. | 3788-44-1 | SDF | |
| 分子式 | C15H18N4O4 | 分子量 | 318.33 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1414 mL | 15.707 mL | 31.4139 mL |
| 5 mM | 0.6283 mL | 3.1414 mL | 6.2828 mL |
| 10 mM | 0.3141 mL | 1.5707 mL | 3.1414 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet