Z-Gly-Pro-AMC
(Synonyms: Z-甘氨酰脯氨酸-4-甲基-7-香豆素) 目录号 : GA23817Z-Gly-Pro-AMC是一种荧光底物,容易被脯氨酰内肽酶水解,导致强烈荧光产物7-氨基-4-甲基香豆素生成(Λex=380 nm,Λem=465 nm)。
Cas No.:68542-93-8
Sample solution is provided at 25 µL, 10mM.
Z-Gly-Pro-AMC functions as a fluorogenic substrate that is susceptible to hydrolysis by prolyl endopeptidase, resulting in the production of intensely fluorescent 7-amido-4-methylcoumarin. (Λex=380 nm, Λem=465 nm) [1-3].
The substrate Z-Gly-Pro-AMC is specifically cleaved by FAPα, which possesses a postprolyl peptidase activity, capable of targeting N-terminal benzyloxycarbonyl (Z)-blocked peptides. This enzymatic action results in the generation of highly fluorescent 7-amido-4-methylcoumarin[4].
References:
[1]. Bracke A, Van Elzen R, et,al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160. doi: 10.1016/j.cca.2019.04.063. Epub 2019 Apr 11. PMID: 30981844.
[2]. Fu P, Sun W, et,al. Identification of two isoforms of Pop in the domestic silkworm, Bombyx mori: Cloning, characterization and expression analysis. Gene. 2018 Aug 15;667:101-111. doi: 10.1016/j.gene.2018.05.021. Epub 2018 May 9. PMID: 29753046.
[3]. Lee KN, Jackson KW, et,al. Antiplasmin-cleaving enzyme is a soluble form of fibroblast activation protein. Blood. 2006 Feb 15;107(4):1397-404. doi: 10.1182/blood-2005-08-3452. Epub 2005 Oct 13. PMID: 16223769.
[4].Huang S, Zhang Y, et,al. Toxicological profile and safety pharmacology of a single dose of fibroblast activation protein-α-based doxorubicin prodrug: in-vitro and in-vivo evaluation. Anticancer Drugs. 2018 Mar;29(3):253-261. doi: 10.1097/CAD.0000000000000593. PMID: 29346131.
Z-Gly-Pro-AMC是一种荧光底物,容易被脯氨酰内肽酶水解,导致强烈荧光产物7-氨基-4-甲基香豆素生成(Λex=380 nm,Λem=465 nm)[1-3]。底物Z-Gly-Pro-AMC被FAPα特异性切割,FAPα具有后脯氨酸肽酶活性,能够靶向n端苯氧羰基(Z)阻断肽。这种酶的作用导致产生高荧光的7-氨基-4-甲基香豆素[4]。
Protocol for assay conditions for the kinetic measurements[1]: |
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Protocol for FAP activity measurements[1]: |
This protocol only provides a guideline, and should be modified according to your specific needs. |
References: [1]. Bracke A, Van Elzen R, et,al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160. doi: 10.1016/j.cca.2019.04.063. Epub 2019 Apr 11. PMID: 30981844. |
Cas No. | 68542-93-8 | SDF | |
别名 | Z-甘氨酰脯氨酸-4-甲基-7-香豆素 | ||
分子式 | C25H25N3O6 | 分子量 | 463.49 |
溶解度 | DMSO : 125 mg/mL (269.70 mM; Need ultrasonic) | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.1575 mL | 10.7877 mL | 21.5754 mL |
5 mM | 0.4315 mL | 2.1575 mL | 4.3151 mL |
10 mM | 0.2158 mL | 1.0788 mL | 2.1575 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet