Z-Gly-Pro-pNA
(Synonyms: Carbobenzoxy-glycyl-L-prolyl-p-Nitroanilide, N-CBZ-Glycyl-L-proline 4-Nitroanilide, Z-Gly-Pro-4-Nitroanilide) 目录号 : GA23825
Z-Gly-Pro-pNA 作为显色底物，可被循环酶二肽基肽酶 IV (DPP IV) 裂解。
Cas No.:65022-15-3
Sample solution is provided at 25 µL, 10mM.
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Gly-Pro-pNA, as a chromogenic substrate, can be cleaved by the circulating enzyme, dipeptidyl peptidase IV (DPP IV).[1]
In vitro, when Z-Gly-Pro-pNA was used as a substrate, SNA-8073-B inhibited prolyl endopeptidase of Flavobacterium non-competitively with IC50 of 8.9 µM.[2] In addition, when Z-Gly-Pro-pNA was used as a substrate, Propeptin inhibited prolyl endopeptidase of the genus Flavobacterium competitively with Ki of 0.70 µM.[3] In addition, using Gly-Pro-p-nitroanilide as substrate to determine the enzymatic activity of this molecule in serum. Using Gly-Pro-pNA as substrate can assess the enzymatic activity and biochemical status of dipeptidyl peptidase IV in patients with rheumatoid arthritis.[4] In vitro, using Gly-Pro-pNA as a substrate can determine DPP-IV inhibitory activities of quinoa protein hydrolysates. Twenty quinoa-derived peptides were determined with in vitro DPP-IV inhibitory activities with IC50 values less than 500 µM. [5]
[1] Fu P, et al. Identification of two isoforms of Pop in the domestic silkworm, Bombyx mori: Cloning, characterization and expression analysis. Gene. 2018 Aug 15;667:101-111.
Kimura K, et al. SNA-8073-B, a new isotetracenone antibiotic inhibits prolyl endopeptidase. I. Fermentation, isolation and biological properties. J Antibiot (Tokyo). 1997 Apr;50(4):291-6.
Kimura K, et al. Propeptin, a new inhibitor of prolyl endopeptidase produced by Microbispora. I. Fermentation, isolation and biological properties. J Antibiot (Tokyo). 1997 May;50(5):373-8.
Yeganeh F, et al. Association of CD26/dipeptidyl peptidase IV mRNA level in peripheral blood mononuclear cells with disease activity and bone erosion in rheumatoid arthritis. Clin Rheumatol. 2018 Dec;37(12):3183-3190.
You H, et al. Preparation and identification of dipeptidyl peptidase IV inhibitory peptides from quinoa protein. Food Res Int. 2022 Jun;156:111176.
References:
Z-Gly-Pro-pNA 作为显色底物，可被循环酶二肽基肽酶 IV (DPP IV) 裂解。[1]
在体外，当以Z-Gly-Pro-pNA为底物时，SNA-8073-B非竞争性抑制黄杆菌的脯氨酰内肽酶，IC50为8.9 µM.[2] 此外，当Z -以Gly-Pro-pNA为底物，Propeptin竞争性抑制黄杆菌属的脯氨酰内肽酶，Ki为0.70 µM.[3] 此外，使用Gly-Pro-p-nitroanilide作为底物来确定该分子在血清中的酶活性。以Gly-Pro-pNA为底物可评估类风湿性关节炎患者体内二肽基肽酶IV的酶活性和生化状态。[4] 在体外，以Gly-Pro-pNA为底物可测定藜麦蛋白水解物的 DPP-IV 抑制活性。二十种藜麦来源的肽经测定具有体外 DPP-IV 抑制活性，IC50 值小于 500 µM。 [5]
| Cas No. | 65022-15-3 | SDF | |
| 别名 | Carbobenzoxy-glycyl-L-prolyl-p-Nitroanilide, N-CBZ-Glycyl-L-proline 4-Nitroanilide, Z-Gly-Pro-4-Nitroanilide | ||
| 分子式 | C21H22N4O6 | 分子量 | 426.43 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3451 mL | 11.7253 mL | 23.4505 mL |
| 5 mM | 0.469 mL | 2.3451 mL | 4.6901 mL |
| 10 mM | 0.2345 mL | 1.1725 mL | 2.3451 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet