Galactose 1-phosphate
(Synonyms: 半乳糖-1-磷酸) 目录号 : GC30337半乳糖1磷酸是半乳糖-1-磷酸尿苷酰转移酶(GALT)的作用底物,具有细胞毒性,对糖代谢途径中的多种酶有抑制作用.Gal-1-P(1- 16mm;37℃;1h)能刺激大鼠脑Na+,K+-ATP酶。
Cas No.:2255-14-3
Sample solution is provided at 25 µL, 10mM.
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Galactose 1-phosphate (Gal-1-P) is the substrate of galactose 1-phosphate uridylyltransferase (GALT), which is cytotoxic and has inhibitory effect on many enzymes in glucose metabolism pathway[1,2].Gal-1-P(1-16 mM;37℃；1h) can stimulated rat brain Na+,K+-ATPase[3].
Gal-1-P (5-10 mM) in culture medium for 7-14 days significantly decreased IGF-1, PDGF and FBS-stimulated DNA synthesis in Fibroblasts[4]. Various concentrations (1-16 mM) of galactose-1-phosphate (Gal-1-P) were preincubated with brain homogenates of suckling rats as well as with pure eel Electroforus electricus AChE at 37℃ for 1 h. Gal-1-P decreased gradually the enzyme activity reaching a plateau, when incubated with 8-16 mM. Gal-1-P can affect acetylcholine degradation acting directly on AChE molecule[5].
References:
[1]. Oh SL, Cheng LY, et,al. Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK. J Inherit Metab Dis. 2020 May;43(3):529-539. doi: 10.1002/jimd.12198. Epub 2019 Dec 17. PMID: 31774565.
[2]. Lai K, Langley SD, et,al. GALT deficiency causes UDP-hexose deficit in human galactosemic cells. Glycobiology. 2003 Apr;13(4):285-94. doi: 10.1093/glycob/cwg033. Epub 2003 Jan 3. PMID: 12626383.
[3]. Tsakiris S, Marinou K, et,al. The effect of galactose metabolic disorders on rat brain Na+,K+-ATPase activity. Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):939-43. doi: 10.1515/znc-2002-9-1030. PMID: 12440737.
[4]. Al-Essa M, Dhaunsi G. Receptor-mediated attenuation of insulin-like growth factor-1 activity by galactose-1-phosphate in neonate skin fibroblast cultures: Galactosemia pathogenesis. Adv Clin Exp Med. 2020 Apr;29(4):499-504. doi: 10.17219/acem/111807. PMID: 32356415.
[5]. Tsakiris S, Schulpis KH. The effect of galactose metabolic disorders on rat brain acetylcholinesterase activity. Z Naturforsch C J Biosci. 2000 Sep-Oct;55(9-10):852-5. doi: 10.1515/znc-2000-9-1032. PMID: 11098845.
半乳糖1磷酸是半乳糖-1-磷酸尿苷酰转移酶(GALT)的作用底物,具有细胞毒性,对糖代谢途径中的多种酶有抑制作用[1,2].
Gal-1-P(1- 16mm;37℃;1h)能刺激大鼠脑Na+,K+-ATP酶[3]。Gal-1-P (5-10 mM；7-14天)在培养基中显著降低成纤维细胞中IGF-1、PDGF和FBS刺激的DNA合成[4]。
将不同浓度(1 ~ 16 mM)的半乳糖-1-磷酸(Gal-1-P)与乳鼠脑匀浆及纯电鳗乙酰胆碱(AChE)在37℃下孵育1 h, Gal-1-P在8 ~ 16 mM孵育1 h后, 酶活性逐渐降低至稳定。并且Gal-1-P可直接作用于乙酰胆碱分子[5]。
| Cell experiment [1]: | |
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Cell lines |
Fibroblasts derived from the foreskin of 3-8-day-old healthy neonates |
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Preparation Method |
Galactose 1-phosphate (Gal-1-P) at different concentrations (1-16 mM) was preincubated separately with whole brain homogenates at 37℃ for 1 h. |
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Reaction Conditions |
0-10 mM；1-14 days |
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Applications |
Gal-1-P can stimulated rat brain Na+,K+-ATPase. |
| Animal experiment [2]: | |
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Animal models |
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Preparation Method |
Cells were cultured for 1-14 days with 0-10 mM Gal-1-P and then stimulated with 5% fetal bovine serum (FBS) or 50 ng/mL of platelet-derived growth factor (PDGF) or fibroblast growth factor (FGF) or IGF-1 for 24 h. |
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Dosage form |
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Applications |
Gal-1-P (5-10 mM) in culture medium for 7-14 days significantly decreased IGF-1-, PDGF-and FBS-stimulated DNA synthesis. |
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References: [1]. Tsakiris S, Marinou K,et,al. The effect of galactose metabolic disorders on rat brain Na+,K+-ATPase activity. Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):939-43. doi: 10.1515/znc-2002-9-1030. PMID: 12440737. |
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| Cas No. | 2255-14-3 | SDF | |
| 别名 | 半乳糖-1-磷酸 | ||
| Canonical SMILES | O=P(O)(O)O[C@H]1O[C@@H]([C@H](O)[C@H](O)[C@H]1O)CO | ||
| 分子式 | C6H13O9P | 分子量 | 260.14 |
| 溶解度 | Water:125 mg/mL (371.67 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8441 mL | 19.2204 mL | 38.4408 mL |
| 5 mM | 0.7688 mL | 3.8441 mL | 7.6882 mL |
| 10 mM | 0.3844 mL | 1.922 mL | 3.8441 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet