(R)-(-)-Rolipram
(Synonyms: (4R)-4-[3-(环戊氧基)-4-甲氧基苯基]吡咯-2-酮,(R)-Rolipram; (-)-Rolipram) 目录号 : GC34984
A PDE4 inhibitor
Cas No.:85416-75-7
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
(–)-Rolipram is a phosphodiesterase 4 (PDE4) inhibitor (IC50 = 0.22 ?M for the guinea pig eosinophil enzyme) and an enantiomer of (±)-rolipram .1 It enhances isoprenaline-induced cAMP accumulation in isolated guinea pig eosinophils (EC50 = 0.19 ?M). (–)-Rolipram (1-1,000 ?M) inhibits TNF and INF-γ production in L1402 CD4+ encephalitogenic rat T cells.2 It inhibits ovalbumin-induced contraction of tracheal rings isolated from ovalbumin-sensitized guinea pigs when used at a concentration of 1 ?M.3 (–)-Rolipram inhibits ovalbumin-induced bronchoconstriction in guinea pigs (ED50 = 0.25 mg/kg).
1.Souness, J.E., and Scott, L.C.Stereospecificity of rolipram actions on eosinophil cyclic AMP-specific phosphodiesteraseBiochem. J.291(Pt 1)(Pt 2)389-395(1993) 2.Sommer, N., L?shmann, P.-A., Northoff, G.H., et al.The antidepressant rolipram suppresses cytokine production and prevents autoimmune encephalomyelitisNat. Med.1(3)244-248(1995) 3.Underwood, D.C., Osborn, R.R., Novak, L.B., et al.Inhibition of antigen-induced bronchoconstriction and eosinophil infiltration in the guinea pig by the cyclic AMP-specific phosphodiesterase inhibitor, rolipramJ. Pharmacol. Exp. Ther.266(1)306-313(1993)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6319 mL | 18.1594 mL | 36.3187 mL |
| 5 mM | 0.7264 mL | 3.6319 mL | 7.2637 mL |
| 10 mM | 0.3632 mL | 1.8159 mL | 3.6319 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。