25-Hydroxy VD2-D6
(Synonyms: 25-羟基维生素D2-D6) 目录号 : GC35082
An internal standard for the quantification of 25-hydroxy vitamin D2
Cas No.:1262843-46-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
25-hydroxy Vitamin D2-d6 is intended for use as an internal standard for the quantification of 25-hydroxy vitamin D2 by GC- or LC-MS. 25-hydroxy Vitamin D2 is a metabolite of vitamin D2 .1 It is formed from vitamin D2 by the cytochrome P450 (CYP) isoforms CYP2R1 and CYP27A1, which have 25-hydroxylase activity, and is bound to vitamin D binding protein (DBP) and albumin in the liver, then secreted into the bloodstream.2 25-hydroxy Vitamin D2 is transported to the kidney, where it is preferentially 24-hydroxylated by CYP24A1 to produce 24,25-dihydroxy vitamin D2.2 It can also be hydroxylated by CYP27B1 to produce 1,25-dihydroxy vitamin D2.2,1 Serum levels of 25-hydroxy vitamin D2 have been used as a marker of vitamin D status.3
1.DeLuca, H.F., Sicinski, R.P., Tanaka, Y., et al.Biological activity of 1,25-dihydroxyvitamin D2 and 24-epi-1,25-dihydroxyvitamin D2Am. J. Physiol.254(4 Pt 1)E402-406(1998) 2.Bikle, D.Vitamin D: Production, metabolism, and mechanisms of actionEndotext(2000) 3.Saenger, A.K., Laha, T.J., Bremner, D.E., et al.Quantification of serum 25-hydroxyvitamin D2 and D3 using HPLC-tandem mass spectrometry and examination of reference intervals for diagnosis of vitamin D deficiencyAm. J. Clin. Pathol.125(6)914-920(2006)
| Cas No. | 1262843-46-8 | SDF | |
| 别名 | 25-羟基维生素D2-D6 | ||
| Canonical SMILES | O[C@@H](CCC/1=C)CC1=C\C=C2[C@@](CC[C@@H]3[C@@H](/C=C/[C@H](C)C(C([2H])([2H])[2H])(C([2H])([2H])[2H])O)C)([H])[C@]3(C)CCC\2 | ||
| 分子式 | C28H38D6O2 | 分子量 | 418.68 |
| 溶解度 | DMSO : 100 mg/mL (238.85 mM; Need ultrasonic) | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3885 mL | 11.9423 mL | 23.8846 mL |
| 5 mM | 0.4777 mL | 2.3885 mL | 4.7769 mL |
| 10 mM | 0.2388 mL | 1.1942 mL | 2.3885 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。