Alisol B

Alisol B is a triterpene that has been found in A. orientale and has diverse biological activities.^1,2,3 It increases the number of LC3 puncta, a marker of autophagy, as well as induces cell cycle arrest at the G₁ phase and apoptosis, in MCF-7 breast cancer cells when used at a concentration of 30 ?M.¹ Alisol B (0.5-5 ?M) inhibits RANKL-induced osteoclast differentiation of mouse bone marrow-derived macrophages (BMDMs).² It reduces increases in ear thickness in a mouse model of delayed-type hypersensitivity induced by picryl chloride when administered at doses of 10 or 50 mg/kg.³

1.Law, B.Y.K., Wang, M., Ma, D.-L., et al.Alisol B, a novel inhibitor of the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump, induces autophagy, endoplasmic reticulum stress, and apoptosisMol. Cancer Ther.9(3)718-730(2010) 2.Lee, J.-W., Kobayashi, Y., Nakamichi, Y., et al.Alisol-B, a novel phyto-steroid, suppresses the RANKL-induced osteoclast formation and prevents bone loss in miceBiochem. Pharmacol.80(3)352-361(2010) 3.Lee, J.H., Kwon, O.S., Jin, H.-G., et al.The rhizomes of Alisma orientale and alisol derivatives inhibit allergic response and experimental atopic dermatitisBiol. Pharm. Bull.35(9)1581-1587(2012)