Dobutamine hydrochloride
(Synonyms: 盐酸多巴酚丁胺) 目录号 : GC35891
A β-
Cas No.:49745-95-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Dobutamine is a catecholamine that acts as a β-
1.Tuttle, R.R., and Mills, J.Dobutamine: Development of a new catecholamine to selectively increase cardiac contractilityCirc. Res.36(1)185-196(1975) 2.Deighton, N.M., Motomura, A., Bals, S., et al.Characterization of theβadrenoceptor subtype(s) mediating the positive inotropic effects of epinine, dopamine, dobutamine, denopamine and xamoterol in isolated human right atriumJ. Pharmacol. Exp. Ther.262(2)532-538(1992) 3.Brown, L., N?bauer, M., and Erdmann, E.Dobutamine: Positive inotropy by nonselective adrenoceptor agonism in isolated guinea pig and human myocardiumNaunyn Schmiedebergs Arch. Pharmacol.335(4)385-390(1987) 4.Innocenti, A., Gül?in, I., Scozzafava, A., et al.Carbonic anhydrase inhibitors. Antioxidant polyphenols effectively inhibit mammalian isoforms I-XVBioorg. Med. Chem. Lett.20(17)5050-5053(2010)
| Cas No. | 49745-95-1 | SDF | |
| 别名 | 盐酸多巴酚丁胺 | ||
| Canonical SMILES | OC1=CC=C(CCNC(C)CCC2=CC=C(O)C=C2)C=C1O.[H]Cl | ||
| 分子式 | C18H24ClNO3 | 分子量 | 337.84 |
| 溶解度 | DMSO: ≥ 33 mg/mL (97.68 mM); Water: 20 mg/mL (59.20 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.96 mL | 14.7999 mL | 29.5998 mL |
| 5 mM | 0.592 mL | 2.96 mL | 5.92 mL |
| 10 mM | 0.296 mL | 1.48 mL | 2.96 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Possible inhibition mechanism of Dobutamine hydrochloride as potent inhibitor for human glucose-6-phosphate dehydrogenase enzyme
J Biomol Struct Dyn 2022 Jan;40(1):204-212.PMID:32835622DOI:10.1080/07391102.2020.1811155.
Glucose-6-phosphate dehydrogenase (G6PD) is the first rate-limiting enzyme in the pentose phosphate pathway. One of the enzyme's most important functions is the production of a reducing agent that is essential for preserving the level of reduced glutathione (GSH). However, some chemicals, such as industrial waste and the active ingredients of several drugs, can cause reduction or blockage in this enzyme's activity. This case causes the occurrence of anemia by damaging erythrocytes. In this study, the G6PD enzyme was purified 21,981 fold with affinity chromatography and the effects of the active ingredients of some antiarrhythmic drugs on enzyme activity were investigated with in vitro and in silico methods. We found that Dobutamine hydrochloride significantly decreased enzyme activity and its inhibitory constant (Ki) value was calculated as 19.02 ± 4.83 mM. The in vitro study results also show that Dobutamine hydrochloride is a potent inhibitor of enzyme activity. We also found that Dobutamine hydrochloride inhibits the hG6PD enzyme's activity by causing structural alterations in substrate and coenzyme binding sites.Communicated by Ramaswamy H. Sarma.
Determination of Dobutamine hydrochloride by enzymatic catalytic spectrofluorimetry
Luminescence 2014 Feb;29(1):92-5.PMID:23616473DOI:10.1002/bio.2509.
A highly sensitive and simple spectrofluorimetric method for the determination of Dobutamine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine was developed. The relationship between the concentration of Dobutamine hydrochloride and the fluorescence quenching (ΔF) of the system is linear under the optimal experimental conditions. The calibration graph is linear in the range 2.00 × 10(-7) to 3.00 × 10(-6) g/mL with a limit of detection of 4.83 × 10(-9) g/mL. This method can be used for the determination of Dobutamine hydrochloride in its pharmaceutical formulations and in urine with satisfactory results.
Effects of Dobutamine hydrochloride on cardiovascular function in horses anesthetized with isoflurane with or without acepromazine maleate premedication
Am J Vet Res 2016 Dec;77(12):1318-1324.PMID:27901396DOI:10.2460/ajvr.77.12.1318.
OBJECTIVE To determine the effects of acepromazine maleate premedication on cardiovascular function before and after infusion of Dobutamine hydrochloride for 30 minutes in isoflurane-anesthetized horses. ANIMALS 6 healthy adult horses. PROCEDURES Each horse was anesthetized once following premedication with acepromazine (0.02 mg/kg, IV) administered 30 minutes prior to anesthetic induction (ACP+ treatment) and once without premedication (ACP- treatment). Anesthesia was induced with IV administration of xylazine hydrochloride (0.8 mg/kg), ketamine hydrochloride (2.2 mg/kg), and diazepam (0.08 mg/kg). Horses were positioned in right lateral recumbency, and anesthesia was maintained via inhalation of isoflurane delivered in oxygen. End-tidal isoflurane concentration was adjusted to achieve a target mean arterial blood pressure of 60 mm Hg (interquartile range [25th to 75th percentile], 57 to 63 mm Hg) for at least 15 minutes. Cardiac index, oxygen delivery index, and femoral arterial blood flow indices were determined 60 minutes after anesthetic induction (baseline). Dobutamine was then infused to achieve a target mean arterial blood pressure of 80 mm Hg (interquartile range, 76 to 80 mm Hg). Data collection was repeated 30 minutes after the start of dobutamine infusion for comparison with baseline values. RESULTS Complete data sets were available from 5 of the 6 horses. Dobutamine administration resulted in significant increases in oxygen delivery and femoral arterial blood flow indices but no significant change in cardiac index for each treatment. However, at baseline or 30 minutes after the start of dobutamine infusion, findings for the ACP+ and ACP- treatments did not differ. CONCLUSIONS AND CLINICAL RELEVANCE In isoflurane-anesthetized horses, dobutamine administration increased oxygen delivery and femoral arterial blood flow indices, but these changes were unaffected by premedication with acepromazine.
Stability of Dobutamine hydrochloride and verapamil hydrochloride in 0.9% sodium chloride and 5% dextrose injections
Am J Hosp Pharm 1984 Apr;41(4):686-9.PMID:6720710doi
The stability of Dobutamine hydrochloride (250 micrograms/ml) and verapamil hydrochloride (160 micrograms/ml) alone and in combination in 0.9% sodium chloride injection or 5% dextrose injection was studied. Solutions were stored both in plastic i.v. bags and in amber-colored glass bottles at 24 degrees C and 5 degrees C for up to seven days. Before storage and at various times during storage, solutions were assayed at least in triplicate by high-performance liquid chromatography, pH was recorded, and visual appearance was noted. All solutions tested under all conditions retained at least 90% potency for seven days. In plastic i.v. bags, dobutamine either alone or in combination with verapamil in both diluents turned a light-pink color in 24 hours at 24 degrees C. The intensity of the pink color increased with time in 0.9% sodium chloride injection; in 5% dextrose injection, solutions, became clear in 48 hours. The pH of solutions prepared in plastic i.v. bags in 5% dextrose injection decreased from 4.0 to 3.1 during the seven-day period at 24 degrees C; results for solutions in amber bottles were similar. At 5 degrees C, the pH and clarity of all solutions in bags and bottles remained stable for seven days. At the concentrations tested, Dobutamine hydrochloride combined with verapamil hydrochloride is stable in 0.9% sodium chloride injection and 5% dextrose injection for 48 hours at 24 degrees C and for seven days at 5 degrees C.
Stability of Dobutamine hydrochloride in selected large-volume parenterals
Am J Hosp Pharm 1982 Nov;39(11):1923-5.PMID:7148861doi
Stability of Dobutamine hydrochloride when mixed with large-volume parenteral solutions was assessed. Dobutamine hydrochloride was added to large-volume solutions of 5% dextrose injection, 0.9% sodium chloride injection, lactated Ringer's injection, and 5% dextrose and 0.45% sodium chloride injection, in both glass and polyvinyl chloride containers; the initial concentration was 1 mg/ml. After 0.25, 1, 3, 8, 24, and 48 hours, the concentration of Dobutamine hydrochloride was determined by high-pressure liquid chromatography assay, and each solution was visually examined for evidence of haze, precipitation, color change, or evolution of gas. Concentration of Dobutamine hydrochloride in the samples did not exhibit any appreciable change over the 48-hour period, and no HPLC peaks indicating degradation products were noted. Color changes were observed in some of the solutions, but no other visual changes occurred. There were no apparent differences in stability between the admixtures packaged in glass and those in polyvinyl chloride bags. At the concentration studied, Dobutamine hydrochloride is stable in the admixtures tested for a minimum of 48 hours.