Endothelin-2, human
(Synonyms: 内皮素2,Endothelin-2 (human, canine); Human endothelin-2) 目录号 : GC35987
A peptide vasoconstrictor
Cas No.:123562-20-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Endothelin-2 is a peptide vasoconstrictor and ligand for the endothelin (ET) receptors ETA and ETB (Kbs= 32.8 and 1.33 nM, respectively).1 It induces β-arrestin recruitment in CHO-K1 cells expressing human ETA and ETB (pD2s =8.75 and 8.55, respectively). Ex vivo, endothelin-2 induces vformal asoconstriction in isolated rat mesenteric arterial beds (ED50 = 90.8 pmol).2 Endothelin-2 also induces contraction of isolated rat uteri, an effect which is reversed by the ETA antagonist BQ-123 but not the ETB antagonist BQ-788 .3 Infusion of endothelin-2 restores vasoconstriction of vessels at the follicular apex of the ovarian follicle and ovulation in Amhr2cre/+Smo2 mice.4
1.Maguire, J.J., Kuc, R.E., Pell, V.R., et al.Comparison of human ETA and ETB receptor signalling via G-protein and β-arrestin pathwaysLife Sci.91(13-14)544-549(2012) 2.Douglas, S.A., and Hiley, C.R.Endothelium-dependent vascular activities of endothelin-like peptides in the isolated superior mesenteric arterial bed of the ratBr. J. Pharmacol.101(1)81-88(1990) 3.Kousides, M., Story, M.E., and Pennefather, J.N.Endopeptidase 24.11 inhibition does not modify uterotonic effects of endothelins in rat uterusPeptides19(9)1585-1593(1998) 4.Migone, F.F., Cowan, R.G., Williams, R.M., et al.In vivo imaging reveals an essential role of vasoconstriction in rupture of the ovarian follicle at ovulationProc. Natl. Acad. Sci. USA113(8)2294-2299(2016)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3926 mL | 1.9632 mL | 3.9263 mL |
| 5 mM | 0.0785 mL | 0.3926 mL | 0.7853 mL |
| 10 mM | 0.0393 mL | 0.1963 mL | 0.3926 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。