Ganoderic acid G
(Synonyms: 灵芝酸G) 目录号 : GC36118
A triterpenoid with anticancer and anti-inflammatory activities
Cas No.:98665-22-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Ganoderic acid G is a triterpenoid that has been found in G. lucidum and has anticancer and anti-inflammatory activities.1,2 It inhibits the proliferation of Meth A sarcoma cells (IC50 = 6.8 ?g/ml).1 Ganoderic acid G inhibits the production of nitric oxide (NO) in LPS-induced BV-2 microglial cells (IC50 = 5.77 ?M).2
1.Min, B.S., Gao, J.J., Nakamura, N., et al.Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against meth-A and LLC tumor cellsChem. Pharm. Bull. (Tokyo)48(7)1026-1033(2000) 2.Yang, J., Ting, X., Zou, L.-H., et al.Lanostane triterpenoids from Ganoderma curtisii and their NO production inhibitory activities of LPS-induced microgliaBioorg. Med. Chem. Lett.26(15)3556-3561(2018)
| Cas No. | 98665-22-6 | SDF | |
| 别名 | 灵芝酸G | ||
| Canonical SMILES | CC12C3=C(C(C(O)C1(C(C(C)CC(CC(C)C(O)=O)=O)CC2=O)C)=O)C4(C(C(C)(C(O)CC4)C)CC3O)C | ||
| 分子式 | C30H44O8 | 分子量 | 532.67 |
| 溶解度 | DMSO : 100 mg/mL (187.73 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8773 mL | 9.3867 mL | 18.7733 mL |
| 5 mM | 0.3755 mL | 1.8773 mL | 3.7547 mL |
| 10 mM | 0.1877 mL | 0.9387 mL | 1.8773 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Glycosylation of Ganoderic acid G by Bacillus Glycosyltransferases
Int J Mol Sci 2021 Sep 9;22(18):9744.PMID:34575908DOI:10.3390/ijms22189744.
Ganoderma lucidum is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous Ganoderma triterpenoids have already been identified, rare Ganoderma triterpenoid saponins were recently discovered. To create novel Ganoderma saponins, Ganoderic acid G (GAG) was selected for biotransformation using four Bacillus glycosyltransferases (GTs) including BtGT_16345 from the Bacillus thuringiensis GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the Bacillus subtilis ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3-o-β-glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26-o-β-glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four Bacillus GTs highlights the possibility of novel Ganoderma triterpenoid saponin production in the future.