N6,N6-Dimethyladenosine
(Synonyms: N6,N6-二甲基腺苷) 目录号 : GC36681
A modified nucleoside
Cas No.:2620-62-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
N6,N6-Dimethyladenosine is a modified nucleoside.1 It has been found in tRNA isolated from M. bovis. Dimethylation of adjacent adenosine nucleosides (m62Am62A) is found in E. coli 16S rRNA and is important for the initiation of protein synthesis.2 N6,N6-Dimethyladenosine inhibits the proliferation of L1210 leukemia cells in vitro (IC50 = 0.5 ?g/ml) but does not have antitumor activity in vivo.2 It is also a component of the antibiotic and protein synthesis inhibitor puromycin .
1.Chan, C.T.Y., Chionh, Y.H., Ho, C.-H., et al.Identification of N6,N6-dimethyladenosine in transfer RNA from Mycobacterium bovis Bacille Calmette-GuérinMolecules16(6)5168-5181(2011) 2.Poldermans, B., Van Buul, C.P.J.J., and Van Knippenberg, P.H.Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. II. The effect of the absence of the methyl groups on initiation of protein biosynthesisJ. Biol. Chem.254(18)9090-9093(1979)
| Cas No. | 2620-62-4 | SDF | |
| 别名 | N6,N6-二甲基腺苷 | ||
| Canonical SMILES | OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=CN=C3N(C)C)O1)O)O | ||
| 分子式 | C12H17N5O4 | 分子量 | 295.29 |
| 溶解度 | DMSO: ≥ 125 mg/mL (423.31 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3865 mL | 16.9325 mL | 33.865 mL |
| 5 mM | 0.6773 mL | 3.3865 mL | 6.773 mL |
| 10 mM | 0.3387 mL | 1.6933 mL | 3.3865 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Metabolic turnover and dynamics of modified ribonucleosides by 13C labeling
J Biol Chem 2021 Nov;297(5):101294.PMID:34634303DOI:10.1016/j.jbc.2021.101294.
Tandem mass spectrometry (MS/MS) is an accurate tool to assess modified ribonucleosides and their dynamics in mammalian cells. However, MS/MS quantification of lowly abundant modifications in non-ribosomal RNAs is unreliable, and the dynamic features of various modifications are poorly understood. Here, we developed a 13C labeling approach, called 13C-dynamods, to quantify the turnover of base modifications in newly transcribed RNA. This turnover-based approach helped to resolve mRNA from ncRNA modifications in purified RNA or free ribonucleoside samples and showed the distinct kinetics of the N6-methyladenosine (m6A) versus 7-methylguanosine (m7G) modification in polyA+-purified RNA. We uncovered that N6,N6-Dimethyladenosine (m62A) exhibits distinct turnover in small RNAs and free ribonucleosides when compared to known m62A-modified large rRNAs. Finally, combined measurements of turnover and abundance of these modifications informed on the transcriptional versus posttranscriptional sensitivity of modified ncRNAs and mRNAs, respectively, to stress conditions. Thus, 13C-dynamods enables studies of the origin of modified RNAs at steady-state and subsequent dynamics under nonstationary conditions. These results open new directions to probe the presence and biological regulation of modifications in particular RNAs.