Netropsin dihydrochloride
(Synonyms: 纺锤菌素二盐酸； 纺锤菌素) 目录号 : GC36724
A DNA minor groove binder
Cas No.:18133-22-7
Sample solution is provided at 25 µL, 10mM.
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Netropsin is a DNA minor groove binder originally isolated from S. netropsis.1,2 It binds to A/T rich regions of DNA (Ka = 4.9 x 105 M-1 for poly(dA)poly(dT) DNA).3 Netropsin inhibits topoisomerase II (IC90 = 200 ?M in a cell-free assay) and teniposide-induced crosslinks in nuclei from 935.1 mouse fibrosarcoma cells (IC50 = 65 ?M).4 It is active against several bacteria, including S. aureus, S. typhosa, K. pneumoniae, and A. aerogenes with MIC values of 5, 10, 10, and 5 ?g/ml, respectively.2 Netropsin (75 ?g/ml) inhibits viral plaque formation in Shope fibroma virus- or vaccinia virus-infected BS-C-1 host cells.5 It increases survival in a mouse model of endotoxemia induced by LPS when administered at a dose of 25 mg/kg.6
1.Neidle, S.DNA minor-groove recognition by small moleculesNat. Prod. Rep.18(3)291-309(2001) 2.Finlay, A.C., Hochstein, F.A., Sobin, B.A., et al.Netropsin, a new antibiotic produced by a StreptomycesJ. Am. Chem. Soc.73(1)341-343(1951) 3.Wartell, R.M., Larson, J.E., and Wells, R.D.Netropsin. A specific probe for A-T regions of duplex deoxyribonucleic acidJ. Biol. Chem.249(21)6719-6731(1974) 4.Beerman, T.A., Woynarowski, J.M., Sigmund, R.D., et al.Netropsin and bis-netropsin analogs as inhibitors of the catalytic activity of mammalian DNA topoisomerase II and topoisomerase cleavable complexesBiochim. Biophys. Acta1090(1)52-60(1991) 5.Becker, Y., Asher, Y., and Zakay-Rones, Z.Congocidine and distamycin A, antipoxvirus antibioticsAntimicrob. Agents Chemother.1(6)483-488(1972) 6.Grant, M.A., Baron, R.M., Macias, A.A., et al.Netropsin improves survival from endotoxaemia by disrupting HMGA1 binding to the NOS2 promoterBiochem. J.418(1)103-112(2009)
| Cas No. | 18133-22-7 | SDF | |
| 别名 | 纺锤菌素二盐酸； 纺锤菌素 | ||
| Canonical SMILES | O=C(C1=CC(NC(CNC(N)=N)=O)=CN1C)NC2=CN(C)C(C(NCCC(N)=N)=O)=C2.[H]Cl.[H]Cl | ||
| 分子式 | C18H28Cl2N10O3 | 分子量 | 503.39 |
| 溶解度 | DMSO : 125 mg/mL (248.32 mM; Need ultrasonic); H2O : 20 mg/mL (39.73 mM; Need ultrasonic) | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9865 mL | 9.9327 mL | 19.8653 mL |
| 5 mM | 0.3973 mL | 1.9865 mL | 3.9731 mL |
| 10 mM | 0.1987 mL | 0.9933 mL | 1.9865 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet