Norepinephrine hydrochloride
(Synonyms: 去甲肾上腺素盐酸盐; Levarterenol hydrochloride; L-Noradrenaline hydrochloride) 目录号 : GC36758
A racemic mixture of of L-norepinephrine and D-norepinephrine
Cas No.:329-56-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
Subcutaneous preadipocytes derived from a 38-year old non-diabetic female donor are immortalized with TERT and HPV E6/E7. For the current studies, a stable diploid clone (referred to as clone B) with consistent differentiation capacity is isolated by ring cloning. Cells are grown in preadipocyte PGM2 media. Once cells are confluent, differentiation is induced by incubation in differentiation media consisting of dexamethasone, IBMX, indomethacin, and additional insulin. Cells are differentiated for 10 days. Prior to treatment, media is replaced with PGM2 media for one day and then switched to serum-free media overnight for treatments. Adipocytes are treated for 6 hours with vehicle, Norepinephrine (NE, 10 μM), CGP (10 nM), or Norepinephrine (NE) and CGP[2]. |
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References: [1]. MacGregor DA, et al. Relative efficacy and potency of beta-adrenoceptor agonists for generating cAMP in human lymphocytes. Chest. 1996 Jan;109(1):194-200. |
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(±)-Norepinephrine is a racemic mixture of the endogenous neurotransmitter (–)-norepinephrine and (+)-norepinephrine. It induces cAMP accumulation in rat cerebral cortical membranes when used at concentrations ranging from 1 to 100 ?M.1 (±)-Norepinephrine induces contraction of bovine anterior cerebral, middle cerebral, and internal carotid arterial strips (EC50s = 0.91, 0.92, and 0.87 ?M, respectively) but induces relaxation of bovine posterior cerebral arterial strips (EC50 = 0.95 ?M).2
1.Tadokoro, C., Kiuchi, Y., Yamazaki, Y., et al.Behavioral stimulation without alteration of β and 5-HT receptors and adenylate cyclase activity in rat brain after chronic sertraline administrationPsychopharmacology (Berl.)130(2)124-130(1997) 2.Ayajiki, K., and Toda, N.Isolated bovine cerebral arteries from rostral and caudal regions: Distinct responses to adrenoceptor agonistsEur. J. Pharmacol.191(3)417-425(1990)
| Cas No. | 329-56-6 | SDF | |
| 别名 | 去甲肾上腺素盐酸盐; Levarterenol hydrochloride; L-Noradrenaline hydrochloride | ||
| Canonical SMILES | OC1=CC=C([C@@H](O)CN)C=C1O.Cl | ||
| 分子式 | C8H12ClNO3 | 分子量 | 205.64 |
| 溶解度 | 5 mg/mL in DMSO (ultrasonic and warming and heat to 40°C) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.8629 mL | 24.3143 mL | 48.6287 mL |
| 5 mM | 0.9726 mL | 4.8629 mL | 9.7257 mL |
| 10 mM | 0.4863 mL | 2.4314 mL | 4.8629 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Norepinephrine-induced diuresis in chronically ethanol-treated rats
Alcohol 1986 Sep-Oct;3(5):283-6.PMID:3778642DOI:10.1016/0741-8329(86)90002-9.
Previous research from this laboratory indicated that noradrenergic mechanisms mediate ethanol diuresis. Experiments described here examined changes in sensitivity of these noradrenergic mechanisms in animals chronically treated with ethanol. Norepinephrine hydrochloride (0-12 micrograms intraventricularly) produced dose-dependent diuresis in control (dextrin maltose)- and ethanol (8-11 g/kg/day)-treated rats on the first day of treatment. Tolerance to ethanol diuresis was present after 10 days of ethanol treatment. Lack of responsiveness to norepinephrine-induced diuresis was evident only on the 20th day of treatment in both ethanol and dextrin-maltose groups of rats. These results suggest that norepinephrine does not play a primary role in the development of tolerance to the diuretic action of ethanol.