Specneuzhenide
(Synonyms: 特女贞苷,Nuezhenide) 目录号 : GC37673
An iridoid glycoside with diverse biological activities
Cas No.:449733-84-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Specneuzhenide is an iridoid glycoside originally isolated from L. lucidum with diverse biological activities.1,2,3,4 It reduces neurotoxicity induced by 6-OHDA in SH-SY5Y cells by 49.2% when used at a concentration of 10 μM.1 Specneuzhenide (25, 50, and 100 μM) reduces high glucose-induced levels of cleaved caspase-3 protein and apoptosis in mouse glomerular mesangial cells.2 It increases cell viability and alkaline phosphatase activity in osteoblastic UMR-106 cells.3 Specneuzhenide inhibits hypoxia-induced VEGFA secretion, VEGFA and prolyl hydroxylase 2 (PHD-2) mRNA expression, and protein levels of VEGFA, HIF-1α, and PHD-2 in human acute retinal pigment epithelial-19 (ARPE-19) cells.4 In vivo, specneuzhenide (5 and 10 mg/kg) prevents retinal neovascularization in a rat model of oxygen-induced retinopathy.
1.Sung, S.H., Kim, E.S., Lee, K.Y., et al.A new neuroprotective compound of Ligustrum japonicum leavesPlanta Med.72(1)62-64(2006) 2.Zhang, A.-n., Li, P., Hong, X.-h., et al.Preventive effect of specnuezhenide on high glucose-induced apoptosis in glomerular mesangial cellsZhongguo Shiyan Fangjixue Zazhi21(8)116-119(2015) 3.Huang, Y., Wu, Y., Wu, J., et al.Chemical constituents from Ligustrum lucidum differentially promote bone formation and prevent oxidative damage in osteoblastic UMR-106 cellsLat. Am. J. Pharm.33(2)258-265(2014) 4.Wu, J., Ke, X., Fu, W., et al.Inhibition of hypoxia-induced retinal angiogenesis by specnuezhenide, an effective constituent of Ligustrum lucidum Ait., through suppression of the HIF-1α/VEGF signaling pathwayMolecules21(12)E1756(2016)
| Cas No. | 449733-84-0 | SDF | |
| 别名 | 特女贞苷,Nuezhenide | ||
| Canonical SMILES | C/C=C1[C@@H](C(C(OC)=O)=CO[C@H]/1O[C@]2([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]2O)CO)CC(OC[C@H]3O[C@H]([C@H](O)[C@@H](O)[C@@H]3O)OCCC4=CC=C(O)C=C4)=O | ||
| 分子式 | C31H42O17 | 分子量 | 686.65 |
| 溶解度 | DMSO: ≥ 100 mg/mL (145.63 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4563 mL | 7.2817 mL | 14.5635 mL |
| 5 mM | 0.2913 mL | 1.4563 mL | 2.9127 mL |
| 10 mM | 0.1456 mL | 0.7282 mL | 1.4563 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Specneuzhenide Ameliorate Complete Freund Adjuvant Induced Arthritis in Rats: Involvement of NF-κB and HO-1/Nrf-2 Pathway
J Oleo Sci 2022;71(4):551-561.PMID:35370215DOI:10.5650/jos.ess21413.
Rheumatoid arthritis (RA) is growing autoimmune and inflammatory disease that occur due to self-destruction of immune response inducing joint deformity and bone erosion. During the arthritis inducing the swelling, pain and inflammation in around the joint and other body organs due to injury of tendons and ligaments. Specneuzhenide (SZ) already proved antioxidant and anti-inflammatory effect against the various diseases. In this experimental study, we scrutinized the anti-arthritic effect of SZ against the complete freund adjuvant (CFA) induced arthritic in rats. Subcutaneously injection of CFA was injected into the subplantar region of the left hind paw for induction the arthritis and rats were divided into different groups and rats received the oral administration of SZ (5, 10 and 15 mg/kg) for 28 days. The body weight, paw swelling arthritic score mRNA expression and biochemical parameters were determined at regular time interval. CFA induced arthritic rats treated with SZ significantly (p < 0.001) enhanced the body weight and decreased the paw swelling, arthritic index and organ (spleen and thymus) index, respectively. SZ treated rats significantly (p < 0.001) decreased the hepatic parameter such as SGPT, SGOT and ALP, anti-CII IgG levels (IgG1 and IgG2A) and inflammatory parameters (COX-2 and PGE2). SZ treated rats significantly (p < 0.001) suppressed the level of MDA and increased the level of GSH, SOD and CAT. SZ treated rats suppressed the inflammatory cytokines such as TNF-α, Il-1β, IL-2, IL-6, IL-16, IL-17 and increased the level of IL-10, TGF-β. SZ treated rats significantly (p < 0.001) suppressed the mRNA expression of Nrf2, HO-1 and NF-κB. On the basis of result, we can say that Specneuzhenide protective effect against CFA induced arthritis in rats via alteration of HO-1/Nrf-2 pathway.
Protective Effects of Specneuzhenide on Renal Injury in Rats with Diabetic Nephropathy
Open Med (Wars) 2019 Oct 6;14:740-747.PMID:31637305DOI:10.1515/med-2019-0085.
Background: We aim to investigate the protective effects and potential mechanisms in Specneuzhenide (SPE) on renal injury in rats with diabetic nephropathy (DN). Results: SPE could inhibit the decrease of body weight compared with the model group (P<0.05), and trigger improvement in the renal index (P<0.05). High dose and low dose SPE could trigger a significant decrease in serum IL1β, IL-6 and TNF-α compared with the model group (P<0.05). SPE could attenuate the glomerular lesions in DN rats. SPE induced up-regulation of podocin and CD2AP (P<0.05). Conclusion: SPE showed protective effects on renal injury through attenuating the pathological injury and urine protein. This process may be closely related to the modulation of CD2AP and podocin expression.
An integrated approach to reveal the chemical changes of Ligustri Lucidi Fructus during wine steaming processing
J Pharm Biomed Anal 2021 Jan 30;193:113667.PMID:33181428DOI:10.1016/j.jpba.2020.113667.
Ligustri Lucidi Fructus (LLF) is a traditional Chinese medicine to treat osteopathic and hepatic diseases. Wine steaming is the major processing method for LLF in Chinese Pharmacopoeia, but the chemical changes involved are still unclear. In this study, a research strategy was proposed to reveal the chemical changes during wine steaming processing of LLF. Firstly, in total 104 compounds were tentatively identified using UHPLC/Orbitrap-MS. Secondly, potential chemical changes were revealed by comparing the peak areas of the 104 compounds between LLF and the corresponding wine-steamed LLF (WLLF). The results indicated that iridoid and phenylethanoid esters were readily hydrolyzed during wine steaming processing, while organic acids, flavonoids, and triterpenes were stable. Finally, 7 selected compounds were simultaneously determined in 21 batches of LLF/WLLF samples using a 10-min UPLC/UV method. The contents of 4 esters decreased from 3.64% to 2.71%, and the contents of 3 potential hydrolysis products increased from 0.27% to 0.88% after processing. Our study indicated that hydrolysis of iridoid and phenylethanoid esters such as Specneuzhenide was responsible for the chemical changes during the wine processing of LLF. The proposed strategy could be employed to investigate the chemical changes of other herbs during processing.
Metabolomics approach based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry to identify the chemical constituents of the Traditional Chinese Er-Zhi-Pill
J Sep Sci 2017 Jul;40(13):2713-2721.PMID:28485887DOI:10.1002/jssc.201601425.
Er-Zhi-Pill, which consists of Ligustri lucidi fructus and Ecliptae prostratae herba, is a classical traditional Chinese medicinal formulation widely used as a liver-nourishing and kidney-enriching tonic. To identify the bioactive ingredients of Er-Zhi-Pill and characterize the variation of chemical constituents between co-decoction and mix of individually decocted L. lucidi fructus and E. prostratae herba, a novel metabolomics approach based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in both positive and negative ion modes, was established to comprehensively analyze chemical constituents and probe distinguishable chemical markers. In total, 68 constituents were unambiguously or tentatively identified through alignment of accurate molecular weights within an error margin of 5 ppm, elemental composition and fragmentation characteristics, including eight constituents, which were confirmed by comparing to reference standards. Furthermore, principal component analysis and partial least squares discriminant analysis using Simca-p+ 12.0 software were applied to investigate chemical differences between formulations obtained by co-decoction and a mixture of individual decoctions. Global chemical differences were found in samples of two different decoction methods, and 16 components, including salidroside, Specneuzhenide and wedelolactone, contributed most to the observed differences. This study provides a basic chemical profile for the quality control and further mechanism research of Er-Zhi-Pill.