Tetracycline
(Synonyms: 四环素) 目录号 : GC37769
Tetracycline(TC,四环素)是一种具有口服活性的广谱抗生素，可有效抑制革兰氏阳性菌、革兰氏阴性菌、衣原体、支原体和立克次体等细菌的活性。
Cas No.:60-54-8
Sample solution is provided at 25 µL, 10mM.
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Tetracycline (TC) is a broad-spectrum antibiotic with oral activity. It is effective against a variety of bacteria, including gram-positive and gram-negative bacteria, chlamydia, mycoplasma, and Rickettsia. It is used in the study of infections and can induce gene expression in the corresponding system[1-4].
Tetracycline (6.25-250 μM) was dose-dependent on NO synthetase activity and nitrite formation in J774 cells[5].
Tetracycline treatment delays the onset and slows the progression of diabetes in human amylin/islet amyloid polypeptide (hA/hIAPP) transgenic mice. Chronic oral administration of tetracycline (0.03 mg/ml in drinking water) from weaning enhances glycemic control and extends lifespan in hemizygous mice [6]. In a dog model of acute ischemia, 4mg/kg tetracycline injected 30 min prior to the occlusion improved the functional recovery from stunning of myocardium caused by ischemia[7]. Tetracycline injection at single doses of 1.5, 10, and 20 mg/kg significantly protected mice from a lethal intraperitoneal injection of LPS[8].
References:
[1]. Platt BN, Jacobs CA, et,al. Tetracycline use in treating osteoarthritis: a systematic review. Inflamm Res. 2021 Mar;70(3):249-259. doi: 10.1007/s00011-021-01435-4. Epub 2021 Jan 29. PMID: 33512569.
[2]. Chopra I, Roberts M. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 ; second page, table of contents. doi: 10.1128/MMBR.65.2.232-260.2001. PMID: 11381101; PMCID: PMC99026.
[3]. Roberts MC. Tetracycline therapy: update. Clin Infect Dis. 2003 Feb 15;36(4):462-7. doi: 10.1086/367622. Epub 2003 Jan 28. PMID: 12567304.
[4]. Aiba A, Nakao H. Conditional mutant mice using tetracycline-controlled gene expression system in the brain. Neurosci Res. 2007 Jun;58(2):113-7. doi: 10.1016/j.neures.2007.01.009. Epub 2007 Jan 20. PMID: 17316857.
[5]. D'Agostino P, Arcoleo F, et,al. Tetracycline inhibits the nitric oxide synthase activity induced by endotoxin in cultured murine macrophages. Eur J Pharmacol. 1998 Apr 10;346(2-3):283-90. doi: 10.1016/s0014-2999(98)00046-6. PMID: 9652371.
[6]. Aitken JF, Loomes KM, et,al. Tetracycline treatment retards the onset and slows the progression of diabetes in human amylin/islet amyloid polypeptide transgenic mice. Diabetes. 2010 Jan;59(1):161-71. doi: 10.2337/db09-0548. Epub 2009 Sep 30. PMID: 19794060; PMCID: PMC2797917.
[7]. Kagawa N, Senbonmatsu TA, et,al. Tetracycline protects myocardium against ischemic injury. Front Biosci. 2005 Jan 1;10:608-19. doi: 10.2741/1557. PMID: 15569603.
[8]. Milano S, Arcoleo F, et,al. Intraperitoneal injection of tetracyclines protects mice from lethal endotoxemia downregulating inducible nitric oxide synthase in various organs and cytokine and nitrate secretion in blood. Antimicrob Agents Chemother. 1997 Jan;41(1):117-21. doi: 10.1128/AAC.41.1.117. PMID: 8980766; PMCID: PMC163671.
Tetracycline(TC,四环素)是一种具有口服活性的广谱抗生素，可有效抑制革兰氏阳性菌、革兰氏阴性菌、衣原体、支原体和立克次体等细菌的活性。Tetracycline可用于研究感染。Tetracycline可诱导相应系统的基因表达[1-4]。
Tetracycline(6.25-250 μM)在J774细胞中剂量依赖性地降低了脂多糖刺激诱导的NO合成酶活性和亚硝酸盐的形成[5]。
Tetracycline在人胰淀素/胰岛淀粉样多肽(hA/hIAPP)转基因小鼠中可延缓糖尿病的发生和进展。断奶后长期口服Tetracycline(0.03 mg/ml drinking water)可改善半合子小鼠的血糖控制和寿命[6]。在狗急性缺血模型中，血管闭塞前30分钟注射4mg/kg Tetracycline可改善缺血引起的心肌休克的功能恢复[7]。注射单剂量Tetracycline(1.5, 10 and 20 mg/kg)的小鼠可明显避免腹腔注射致死性LPS[8]。
| Cell experiment [1]: | |
Cell lines | J774 macrophage |
Preparation Method | Cells were cultured in medium stimulated with lipopolysaccharide (1 μg/ml) and treated with varying doses of tetracycline. |
Reaction Conditions | 6.25-250 μM; 6h |
Applications | Tetracycline reduced lipopolysaccharide-stimulated inducible NO synthase activity and nitrite formation in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Adult mongrel dogs (acute ischemia model) |
Preparation Method | Saline or tetracycline (4 mg/kg in saline) was administered intravenously at a volume of 0.5 mL/kg, 30 minutes before the ligation. The ligature around the coronary artery was tightened for 20 minutes and then released to allow reperfusion. |
Dosage form | 4 mg/kg; i.p |
Applications | Tetracycline, injected 30 minutes before the occlusion, enhanced the functional recovery of the myocardium from stunning caused by ischemia. |
References: | |
| Cas No. | 60-54-8 | SDF | |
| 别名 | 四环素 | ||
| Canonical SMILES | O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@]3([H])[C@](C)(O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N | ||
| 分子式 | C22H24N2O8 | 分子量 | 444.43 |
| 溶解度 | DMSO: 125 mg/mL (281.26 mM) | 储存条件 | Store at 2-8°C,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2501 mL | 11.2504 mL | 22.5007 mL |
| 5 mM | 0.45 mL | 2.2501 mL | 4.5001 mL |
| 10 mM | 0.225 mL | 1.125 mL | 2.2501 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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