Thevetiaflavone
(Synonyms: 黄花夹竹桃黄酮,Apigenin-5-methyl ether) 目录号 : GC37780
Thevetiaflavone 可以上调 Bcl?2 的表达，下调 Bax 和 caspase-3 的表达。
Cas No.:29376-68-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Thevetiaflavone could upregulate the expression of Bcl?2 and downregulate that of Bax and caspase?3. Bcl-2 Bax Caspase-3
Thevetiaflavone, a natural flavonoid obtained from Wikstroemia indica, could improve cell viability and suppresses the leakage of lactate dehydrogenase from the cytoplasm. Further investigation of the mechanisms demonstrated that Thevetiaflavone decreases overproduction of ROS and ameliorates ROS?mediated mitochondrial dysfunction, including collapse of mitochondrial membrane potential and mitochondrial permeability transition pore opening. Thevetiaflavone reduces the intracellular Ca2+ level, which is closely associated with mitochondrial function and interplays with ROS. Furthermore, Thevetiaflavone inhibits apoptosis in PC12 cells through upregulating the expression of Bcl?2 and downregulating that of Bax and caspase?3 in addition to increasing the activity of caspase?3. These results further indicate the protective effects of thevetiaflavone in vivo and its application in the clinic[1].
[1]. Yao H, et al. Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS?mediated mitochondrial dysfunction. Mol Med Rep. 2017 Dec;16(6):9197-9202.
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.5179 mL | 17.5895 mL | 35.1791 mL |
| 5 mM | 0.7036 mL | 3.5179 mL | 7.0358 mL |
| 10 mM | 0.3518 mL | 1.759 mL | 3.5179 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。