Timosaponin B III
(Synonyms: 知母皂苷BIII) 目录号 : GC37796
Timosaponin B III 是一种主要的生物活性甾体皂苷，从知母中分离出来，具有抗炎、抗血小板聚集和抗抑郁作用。
Cas No.:142759-74-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Timosaponin B III is a major bioactive steroidal saponin isolated from Anemarrhena asphodeloides Bge, and exhibits anti-inflammatory, anti-platelet aggregative and anti-depressive effects[1][2][3].
[1]. Zhang XL, et al. Timosaponin B-III exhibits antidepressive activity in a mouse model of postpartum depression by the regulation of inflammatory cytokines, BNDF signaling and synaptic plasticity. Exp Ther Med. 2017 Oct;14(4):3856-3861. [2]. Zhao YF, et al. New transformation pathway and cytotoxic derivatives from the acid hydrolysis of timosaponin B III. Nat Prod Res. 2018 Nov 20:1-7. [3]. Kang LP, et al. Steroidal glycosides from the rhizomes of Anemarrhena asphodeloides and their antiplateletaggregation activity. Planta Med. 2012 Apr;78(6):611-6.
| Cas No. | 142759-74-8 | SDF | |
| 别名 | 知母皂苷BIII | ||
| 分子式 | C45H74O18 | 分子量 | 903.06 |
| 溶解度 | DMSO : 100 mg/mL (110.73 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1073 mL | 5.5367 mL | 11.0735 mL |
| 5 mM | 0.2215 mL | 1.1073 mL | 2.2147 mL |
| 10 mM | 0.1107 mL | 0.5537 mL | 1.1073 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
New transformation pathway and cytotoxic derivatives from the acid hydrolysis of Timosaponin B III
Nat Prod Res 2019 Oct;33(19):2755-2761.PMID:30453752DOI:10.1080/14786419.2018.1499640.
Timosaponin B III is a major bioactive steroidal saponin isolated from Anemarrhena asphodeloides Bge. To potentially discover derivatives with better biological activity, Timosaponin B III was structurally modified via acid hydrolysis to yield one new (2, timopregnane A I) C21 steroidal glycoside and seven known compounds. Their structures were elucidated on the basis of NMR spectroscopy and mass spectrometry. All eight compounds were evaluated for cytotoxic activity against MCF7, SW480, HepG2, and SGC7901 cell lines in vitro. As a result, compounds 6 and 7 showed significant activity (IC50 2.94-12.2 μM) against all tested cell lines. Structure-activity relationships of these compounds were investigated and the preliminary conclusions were provided. Moreover, a new transformation pathway was discovered in the acid hydrolysis of Timosaponin B III for the first time.
Steroidal glycosides from the rhizomes of Anemarrhena asphodeloides and their antiplatelet aggregation activity
Planta Med 2012 Apr;78(6):611-6.PMID:22307934DOI:10.1055/s-0031-1298223.
Five new steroidal glycosides, timosaponin J ( 1), timosaponin K ( 2), (25 S)-karatavioside C ( 5), timosaponin L ( 6), and (25 S)-officinalisnin-I ( 8), together with eight known steroidal saponins, timosaponin E (1) ( 3), purpureagitosid ( 4), timosaponin BII ( 7), Timosaponin B III ( 9), anemarrhenasaponin I ( 10), anemarrhenasaponin III ( 11), anemarrhenasaponin A (2) ( 12), and timosaponin A III ( 13), were isolated from the rhizomes of Anemarrhena asphodeloides. Their structures were elucidated on the basis of spectroscopic and chemical evidence. The aglycones of compounds 1 and 2 are new aglycones. Compounds 1- 13 were evaluated for their platelet aggregation activities, and compound 13 exhibited the strongest inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation.
[HPLC-PDA-CAD Fingerprints of Salt Anemarrhenae Rhizoma]
Zhong Yao Cai 2015 May;38(5):942-7.PMID:26767285doi
Objective: To establish the HPLC-PDA-CAD fingerprints and to determine its seven main constituents so as to provide a reliable evidence for the scientific evaluation and quality control of salt Anemarrhenae Rhizoma. Methods: The chromatographic fingerprint was obtained with Thermo Hypersil C18 column (250 mm x 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water; The CAD parameters were pressure of 241. 3 kPa, filter of high and range of 200 pA. The detection wavelength of PDA was set at 258 nm. Results: The common mode of HPLC-PDA-CAD fingerprint of salt Anemarrhenae Rhizoma was set up. There were 5 PDA and 12 CAD common peaks in the fingerprints. Timosaponin B II, anemarsaponin E, Timosaponin B III, timosaponin A III, neomangiferin, mangiferin and baohuoside I were identified in fingerprints and determined. Conclusion: The established HPLC-PDA-CAD fingerprint method is accurate, reliable, and has a good reproducibility and precision, which can be used for the quality control of salt Anemarrhenae Rhizoma.
[Effects of different processing methods on five main chemical constituents of Anemarrhena asphodeloides Bge. studied by high performance liquid chromatography]
Se Pu 2012 Dec;30(12):1271-5.PMID:23593885DOI:10.3724/sp.j.1123.2012.07024.
A high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of neomangiferin, mangiferin, Timosaponin B III, anemarrhenasaponin I and timosaponin A III in the products of Anemarrhena asphodeloides Bge. processed by different methods. By comparing and analyzing the variation of the contents of the five components, the effects of processing on chemical constituents of Anemarrhena asphodeloides Bge. were explored, which provided evidences for the relevance between processing and the property changes of Anemarrhena asphodeloides Bge. The chromatographic separation was performed on an Alltima C18 column (250 mm x 4.6 mm, 5 microm) with a gradient elution of acetonitrile (A) and 0. 1% formic acid (B) (0 - 10 min, 5% A - 20% A; 10 - 15 min, 20% A - 25% A; 15 -30 min, 25% A - 80% A; 30 -35 min, 80% A - 100% A) at a flow rate of 0.8 mL/min. Neomangiferin and mangiferin were detected by an ultraviolet detector at 265 nm and room temperature. Timosaponin B III, anemarrhenasaponin I and timosaponin A III were detected by an evaporative light scattering detector with the drift temperature at 50 degrees C and gas pressure at 179.1 kPa (26 psi). To some extent, the contents of the major components varied in different processed products of Anemarrhena asphodeloides Bge. The results indicated that different processing methods caused significant differences in the contents of the major components of Anemarrhena asphodeloides Bge. It is of great use for further researching the relevance of the processing methods to pharmacodynamics of Anemarrhena asphodeloides Bge.
[Simultaneous determination of flavones and saponins of Rhizoma Anemarrhenae by HPLC-DAD-ELSD]
Zhongguo Zhong Yao Za Zhi 2015 Jan;40(1):108-11.PMID:25993798doi
This study is to establish an HPLC-DAD-ELSD method for simultaneous determination of 5 flavones and saponins in Rhizoma Anemarrhenae including neo-mangiferin, mangiferin, timosaponin B II, Timosaponin B III and timosaponin A III. Samples were analyzed on a Merck Purospher STAR column(4.6 mm x 250 mm, 5 μm). The mobile phase consisted of acetonitrile( A) and 0. 1% formic acid (B) with gradient elution at a flow rate of 1.0 mL · min(-1). The column temperature was set at 40 °C. The DAD detector wavelength was set at 254 nm. The ELSD conditions were as follows: the nebulizing gas flow rate was 2.0 L · min(-1) and temperature of drift tube was 105 °C. The volume was 10 μL. The five compounds were well separated with good linear correlations. The mean recoveries were between 102.0%-104.0%. This method was quick and reliable which provides a foundation for quality control of R. Anemarrhenae.