Tolfenpyrad
(Synonyms: 唑虫酰胺) 目录号 : GC37811
Tolfenpyrad 是一种杀虫剂，于2002 年在日本获得批准上市。
Cas No.:129558-76-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >96.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Tolfenpyrad is a pesticide that was first approved in 2002 in Japan.
[1]. Yamaguchi K, et al. Analysis of tolfenpyrad and its metabolites in plasma in a tolfenpyrad poisoning case. J Anal Toxicol. 2012 Sep;36(7):529-37. [2]. Hikiji W, et al. Acute fatal poisoning with Tolfenpyrad. J Forensic Leg Med. 2013 Nov;20(8):962-4.
| Cas No. | 129558-76-5 | SDF | |
| 别名 | 唑虫酰胺 | ||
| Canonical SMILES | O=C(C1=C(Cl)C(CC)=NN1C)NCC2=CC=C(OC3=CC=C(C)C=C3)C=C2 | ||
| 分子式 | C21H22ClN3O2 | 分子量 | 383.87 |
| 溶解度 | DMSO: ≥ 56 mg/mL (145.88 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.605 mL | 13.0252 mL | 26.0505 mL |
| 5 mM | 0.521 mL | 2.605 mL | 5.2101 mL |
| 10 mM | 0.2605 mL | 1.3025 mL | 2.605 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Sublethal effects of Tolfenpyrad on the development, reproduction, and predatory ability of Chrysoperla sinica
Ecotoxicol Environ Saf 2022 May 1;236:113482.PMID:35367884DOI:10.1016/j.ecoenv.2022.113482.
The lacewing, Chrysoperla sinica, is a predaceous insect that is important in crop pest management. Chemical pesticides have adversely impacted predaceous insect species. Here we studied the effect of Tolfenpyrad on C. sinica. The acute toxicity of Tolfenpyrad to the second-instar larvae was determined and indicated that Tolfenpyrad is a medium-risk insecticide. Sublethal concentrations (LC10, LC20, and LC30) of Tolfenpyrad had effects on the development, reproduction, and predatory ability of C. sinica. When the second-instar larvae of C. sinica were exposed to sublethal concentrations of Tolfenpyrad, the activities of protective enzymes, such as superoxide dismutase, peroxidase, and catalase, and detoxification enzymes, including carboxylesterase, glutathione-S-transferase, and P450 monooxygenases, were increased with exposure time. The second-instar larvae of C. sinica exposed to sublethal concentrations of Tolfenpyrad exhibited an oxidative stress response that increased the levels of malondialdehyde and reactive oxygen species (ROS). Within 48-120 h after treatment, the contents of mitochondrial respiratory chain complex I and adenosine triphosphate in the second-instar larvae were decreased. This resulted in an imbalance between the production and clearance of ROS and caused cellular damage.
Dissipation behavior and risk assessment of Tolfenpyrad from tea bushes to consuming
Sci Total Environ 2022 Feb 1;806(Pt 4):150771.PMID:34619216DOI:10.1016/j.scitotenv.2021.150771.
The dissipation behavior of Tolfenpyrad, a widely used pyrazole insecticide in tea plantations, was investigated during tea bushes growing, manufacturing and brewing. Tolfenpyrad dissipated fast on the tea bushes with the half-lives of 1.8-2.3 days. Manufacturing processes of green tea and black tea further reduced the Tolfenpyrad residue by 3.5%-36.4%. The average processing factors (PFs) of Tolfenpyrad ranged from 0.68 to 1.40 and 0.84 to 1.30 during the processing of green tea and black tea, respectively. Then a low infusion factor of 9.8%-19.9% was observed during the brewing of made tea, as the water solubility of Tolfenpyrad was only 0.087 mg/L. Therefore, more than 96% of the initial deposition of Tolfenpyrad was dissipated and not accessible for consuming. Results of the risk quotient (RQ) assessment also indicated a negligible health risk by tea consumption. Results from this study indicated that the residue of Tolfenpyrad can be reduced by proper field management, manufacturing and brewing processes, where field dissipation and brewing were key steps to minimize its risks. Data of this study could also provide guidance for rational application of Tolfenpyrad in tea plantations.
The influence of Tolfenpyrad on fitness, development, and reproduction in parents and offspring of Coccinella septempunctata
Ecotoxicol Environ Saf 2021 Mar 1;210:111875.PMID:33454577DOI:10.1016/j.ecoenv.2020.111875.
Coccinella septempunctata (ladybird) is one of the foremost natural predators that feed on aphids. Thus, C. septempunctata serves as an effective biological control agent in integrated pest management (IPM) programs. To supplement the activity of biological control agents, IPM programs often incorporate chemical pesticides to bolster crop protection. To evaluate the effects of a potent insecticide, Tolfenpyrad, on C. septempunctata, we tested the sublethal effects of Tolfenpyrad on all developmental stages of the life cycle of C. septempunctata and its effects on the next generation. For sublethal testing of the parent generation, the LR50 of Tolfenpyrad for C. septempunctata was determined to range from 1.04 to 8.43 g a.i. /hm2 within a set exposure period, while the hazard quotient (HQ) values were above our threshold value of 2 during the entire observation period. These data indicated a potential toxicity risk from Tolfenpyrad exposure. The no observed effect application rates (NOERs) of Tolfenpyrad on parents (F0) were determined for survival (0.485 g a.i. /hm2), developmental time of pupation (0.242 g a.i. /hm2), and fecundity (0.485 g a.i. /hm2). Application of sublethal doses to unexposed progeny (F1) of exposed parents, prolonged the L1 (1st instar of larvae) and L2 (2nd instar of larvae) stage, while the total longevity, intrinsic rate of increase (r), finite rate of increase (γ), net reproductive rate (R0), and mean generation time (T) were significantly reduced. These results demonstrated the negative influence of sublethal concentrations of Tolfenpyrad on C. septempunctata and its persistent effects on subsequent generations.
Residue, dissipation and dietary intake risk assessment of Tolfenpyrad in four leafy green vegetables under greenhouse conditions
Food Chem X 2022 Feb 4;13:100241.PMID:35499034DOI:10.1016/j.fochx.2022.100241.
A novel and accurate analytical method for the determination of Tolfenpyrad in four leafy green vegetables, Brassica bara L., Spinacia oleracea L., Lactuca sativa L. and Brassica chinensis L., was developed and applied to investigate the residue distribution and dietary risk under greenhouse conditions. The established approach was determined to be adequate, with recoveries of 79.2%-92.9% and relative standard deviations < 8%. Tolfenpyrad dissipated relatively rapidly in four leafy green vegetables. Terminal residues of Tolfenpyrad were below 0.5 mg/kg (maximum residue limit for Brassica bara L. set by China) in leafy green vegetables collected 28 d after the last application. Due to risk quotient values < 100%, the residue levels of Tolfenpyrad in leafy green vegetables collected 21 days after the last application were deemed safe for consumers. The results provide field data for the reasonable use and dietary risk assessment of Tolfenpyrad in leafy green vegetables.
Effects of Tolfenpyrad exposure on development and response mechanism in the silkworm, Bombyx mori
Pestic Biochem Physiol 2023 Jan;189:105280.PMID:36549810DOI:10.1016/j.pestbp.2022.105280.
Tolfenpyrad is a broad spectrum of insecticide that can effectively kill different types of pests, including Lepidoptera. However, due to improper use, the adverse effects of Tolfenpyrad on beneficial or economic insects have not been well studied. In this study, we systematically investigated the toxic effect of sublethal Tolfenpyrad on silkworms. Sublethal Tolfenpyrad exposure can affect the body weight, developments days, cocooning rate, eclosion rate and pupation rate. To further study the response mechanism of silkworms to Tolfenpyrad stimulation, we compared the different expression genes by transcriptome sequencing and verified them by qRT-PCR. We found that significant changes in the genes expression was involved in xenobiotics biodegradation and metabolism, immune system and digestive system after Tolfenpyrad treatment. To further investigate the possible mechanisms by which intestinal microbia in the response to Tolfenpyrad, we analysed the microbia changes in the midgut of silkworms by 16S rRNA gene sequencing. The results showed that the relative abundances of Enterobacter and Staphylococcus were increased whereas the Tyzzerella and Methylobacterium-Methylorubrum were decreased after Tolfenpyrad stimulation. Taken together, these results indicated that low concentration of Tolfenpyrad affect the growth and development of silkworms. Silkworms respond to the toxicity of Tolfenpyrad by inducing immune and detoxification-related gene expression or altering microbial composition in the midgut.