Transcrocetin meglumine salt
(Synonyms: trans-Crocetin meglumine salt) 目录号 : GC37821
A natural apocarotenoid with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
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Transcrocetin is a natural apocarotenoid isolated from C. sativus and G. jasminoides that has antioxidant, antiproliferative, anti-inflammatory, cardioprotective, and antinociceptive properties.1,2,3,4,5,6 It scavenges 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC50 = 17.8 μg/ml) and inhibits growth of MKN28 stomach, MCF-7 breast, and Caco-2 colon cancer cell lines (IC50s = 53, 63, and 103 μM, respectively).2,3 Transcrocetin (20 μM) protects primary rat microglial cells from LPS-induced death and decreases LPS-induced production of intracellular reactive oxygen species (ROS), TNF-α, IL-1β, and NF-κB.4 Transcrocetin (100 mg/kg) increases the level of glutathione (GSH), catalase (CAT), creatine kinase (CK), and lactate dehydrogenase (LDH) in cardiac tissue in a rat model of myocardial infarction induced by isoproterenol .5 It also increases the pressure threshold and latency to withdrawal in response to mechanical and thermal stimuli, respectively, indicating a decrease in allodynia in a mouse model of spared nerve injury when administered intrathecally at a dose of 30 mg/kg.6
1.Pfister, S.L., Meyer, P., Steck, A., et al.Isolation and structure elucidation of carotenoid?glycosyl esters in gardenia fruits (Gardenia jasminoides Ellis) and saffron (Crocus sativus Linne)J. Agric. Food Chem.44(9)2612-2615(1996) 2.Kanakis, C.D., Tarantilis, P.A., Tajmir-Riahi, H.A., et al.Crocetin, dimethylcrocetin, and safranal bind human serum albumin: Stability and antioxidative propertiesJ. Agric. Food Chem.55(3)970-977(2007) 3.Oliveira, H., Cai, X., Zhang, Q., et al.Gastrointestinal absorption, antiproliferative and anti-inflammatory effect of the major carotenoids of Gardenia jasminoides Ellis on cancer cellsFood Funct.8(4)1672-1679(2017) 4.Nam, K.N., Park, Y.-M., Jung, H.-J., et al.Anti-inflammatory effects of crocin and crocetin in rat brain microglial cellsEur. J. Pharmacol.648(1-3)110-116(2010) 5.Zhang, W., Li, Y., and Ge, Z.Cardiaprotective effect of crocetin by attenuating apoptosis in isoproterenol induced myocardial infarction rat modelBiomed. Pharmacother.93376-382(2017) 6.Wang, X., Zhang, G., Qiao, Y., et al.Crocetin attenuates spared nerve injury-induced neuropathic pain in miceJ. Pharmacol. Sci.135(4)141-147(2017)
Cell experiment: | Cytotoxicity of test compounds is determined by MTT assay using Caco-2 cells in 96 well plates at a density of 20.000 cells per well in 200 ?l FBS-free medium, grown for 96 h and followed by 24 h contact time with the test compounds (100 ?L of serum-free media containing SE 0.5, 1, and 2 mg/mL; trans-crocin-1 250, 500, and 1000 ?M; Transcrocetin 10, 40, 80, and 160 ?M) and incubation at 37°C/5% CO2. The incubation solutions are aspirated, each well is washed twice with 150 ?L of PBS and 50 ?L of MTT solution are added (2.5 mg/mL in PBS). Supernatants are discarded and the formed formazan is dissolved in 50 ?L of DMSO. The absorption of the resulting solution is determined at λ=492 nm against reference wavelength λ=690 nm[1]. |
References: [1]. Lautenschl?ger M, et al. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44. | |
| Cas No. | SDF | ||
| 别名 | trans-Crocetin meglumine salt | ||
| Canonical SMILES | O[C@H]([C@H]([C@@H]([C@@H](CO)O)O)O)CNC.O=C(O)/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C(O)=O.O[C@H]([C@H]([C@@H]([C@@H](CO)O)O)O)CNC | ||
| 分子式 | C34H58N2O14 | 分子量 | 718.83 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3911 mL | 6.9557 mL | 13.9115 mL |
| 5 mM | 0.2782 mL | 1.3911 mL | 2.7823 mL |
| 10 mM | 0.1391 mL | 0.6956 mL | 1.3911 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet