VIP(6-28)(human, rat, porcine, bovine)
目录号 : GC37910
VIP(6-28)(human, rat, porcine, bovine) 是一种有效的外源性血管活性肠肽 (VIP) 拮抗剂。
Cas No.:69698-54-0
Sample solution is provided at 25 µL, 10mM.
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VIP(6-28)(human, rat, porcine, bovine) is an effective antagonist of the actions of exogenous vasoactive intestinal peptide (VIP) on cAMP. VIP[1]
VIP(6-28) is an effective VIP antagonist in the superior cervical ganglion (SCG) , and results obtained using this analog indicate that endogenous VIP can participate in a positive feedback loop in injured sympathetic neurons in which it enhances its own expression. VIP(6-28), when added to short-term cultures of adult SCG at a concentration of 10, 30, or 100 μM, reduces the increase in cAMP levels produced by stimulation with 10 μM VIP by 52, 64, or 81%, respectively. At any of these concentrations tested, VIP(6-28) by itself does not alter cAMP levels. In contrast to its ability to reduce the VIP-stimulated elevation in cAMP levels by 64%, the addition of 30 μM VIP(6-28) to culture medium does not significantly alter cAMP levels measured after stimulation of adult ganglia with either isoproterenol or forskolin (10 μM each). Similar results on the ability of VIP(6-28) to block VIP-stimulated increases in cAMP levels are obtained in neuron-enriched and in non-neuronal cell-enriched dissociated cultures[1].
[1]. Mohney RP, et al. Vasoactive intestinal peptide enhances its own expression in sympathetic neurons after injury. J Neurosci. 1998 Jul 15;18(14):5285-93.
Kinase experiment: | Adult rats are killed by decapitation. The SCGs are removed, desheathed, placed in organ culture, and maintained for 24 or 48 hr in F-12 defined medium equilibrated with 95% O2 and 5% CO2. Some ganglia are preincubated for 30 min in medium containing the VIP receptor antagonist VIP(6-28), and then transferred for 24 hr to medium containing both VIP(6-28) and an agonist. In experiments in which cAMP is to be measured, ganglia are removed from animals and preincubated for 30 min in F-12 medium containing 500 μM IBMX to prevent the metabolism of cAMP. Ganglia are then incubated for an additional 30 min in F-12 medium with IBMX and the compound to be studied. When the action of VIP(6-28) is examined, it is added to the medium during the last 5 min of the preincubation and throughout the incubation. Ascorbic acid (0.2 mg/mL) is added to cultures containing isoproterenol to retard oxidation of the catecholamine. No significant differences in peptide levels are detected between ganglia maintained in F-12 alone and those cultured in medium containing ascorbic acid[1]. |
References: [1]. Mohney RP, et al. Vasoactive intestinal peptide enhances its own expression in sympathetic neurons after injury. J Neurosci. 1998 Jul 15;18(14):5285-93. | |
| Cas No. | 69698-54-0 | SDF | |
| 分子式 | C126H207N37O34S | 分子量 | 2816.28 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3551 mL | 1.7754 mL | 3.5508 mL |
| 5 mM | 0.071 mL | 0.3551 mL | 0.7102 mL |
| 10 mM | 0.0355 mL | 0.1775 mL | 0.3551 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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