Xylotriose
(Synonyms: 木三糖) 目录号 : GC38172
A xylooligosaccharide
Cas No.:47592-59-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Xylotriose is a xylooligosaccharide that has been found in corn and various agricultural wastes.1,2
1.Chapla, D., Pandit, P., and Shah, A.Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probioticsBioresour. Technol.115215-221(2012) 2.Akpinar, O., Erdogan, K., and Bostanci, S.Enzymatic production of xylooligosaccharide from selected agricultural wastesFood Bioprod. Process.87(2)145-151(2009)
| Cas No. | 47592-59-6 | SDF | |
| 别名 | 木三糖 | ||
| Canonical SMILES | O[C@H]([C@H]([C@H](O)CO1)O)[C@]1([H])O[C@H]2[C@@H]([C@H]([C@H](O[C@H](CO)[C@H](O)[C@@H](O)C=O)OC2)O)O | ||
| 分子式 | C15H26O13 | 分子量 | 414.36 |
| 溶解度 | H2O : 125 mg/mL (301.67 mM; Need ultrasonic); DMSO : 100 mg/mL (241.34 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4134 mL | 12.0668 mL | 24.1336 mL |
| 5 mM | 0.4827 mL | 2.4134 mL | 4.8267 mL |
| 10 mM | 0.2413 mL | 1.2067 mL | 2.4134 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Efficient production of xylooligosaccharides rich in xylobiose and Xylotriose from poplar by hydrothermal pretreatment coupled with post-enzymatic hydrolysis
Bioresour Technol 2021 Dec;342:125955.PMID:34547709DOI:10.1016/j.biortech.2021.125955.
A promising approach for production of value-added xylooligosaccharides (XOS) from poplar was developed by combining hydrothermal pretreatment and endo-xylanase post-hydrolysis. Results showed that the 35.4% XOS (DP 2-6) and 17.6% low DP xylans (DP > 6) were obtained at the identified optimal condition (170 °C, 50 min) for hydrothermal pretreatment. Structural features of low DP xylans generated during the hydrothermal pretreatment were examined, revealing that low DP xylans are mainly comprised of 4-O-methylglucuronic xylan and are involved in lignin carbohydrate complexes. Moreover, higher pretreatment intensity promoted the cleavage of side-chain substituents including arabinose and glucuronic acid groups. The subsequent endo-xylanase hydrolysis of the pretreatment liquor hydrolyzed low DP xylans, contributing to a significant improvement in xylobiose and Xylotriose proportions. This combined strategy resulted in a XOS with conversion yield of 44.6% containing 78.7% xylobiose and Xylotriose starting from the initial xylan in raw poplar.
The enhancement of xylose monomer and Xylotriose degradation by inorganic salts in aqueous solutions at 180 degrees C
Carbohydr Res 2006 Nov 6;341(15):2550-6.PMID:16920089DOI:10.1016/j.carres.2006.07.017.
The inorganic salts KCl, NaCl, CaCl2, MgCl2, and FeCl3, and especially the latter, significantly increased xylose monomer and Xylotriose degradation in water heated to 180 degrees C with unaccountable losses of xylose amounting to as high as 65% and 78% for xylose and Xylotriose, respectively, after 20 min incubation with 0.8% FeCl3. Furthermore, losses of both xylose and Xylotriose were well described by first order homogeneous kinetics, and the rate constants for xylose and Xylotriose disappearance increased 6- and 49-fold, respectively, when treated with 0.8% FeCl3 solution compared to treatment with just pressurized hot water at the same temperature. Although the addition of these inorganic salts produced a significant drop in pH, the degradation rates with salts were much faster than could be accounted for by a pH change. For example, the rate constants for the disappearance of xylose and Xylotriose with 0.8% FeCl3 were 3-fold and 7-fold greater, respectively, than for treatment with very dilute sulfuric acid at the same pH. In addition, xylose losses were greater than could be accounted for by just furfural production, suggesting that other degradation products were also formed, and xylose losses to unidentified compounds increased significantly with the addition of FeCl3. The unidentified compounds could be formed through aqueous furfural resinification and condensation reactions that are accelerated by FeCl3, but the actual mechanisms are still not clear.
An endoxylanase rapidly hydrolyzes xylan into major product xylobiose via transglycosylation of xylose to Xylotriose or xylotetraose
Carbohydr Polym 2020 Jun 1;237:116121.PMID:32241400DOI:10.1016/j.carbpol.2020.116121.
Here, we proposed an effective strategy to enhance a novel endoxylanase (Taxy11) activity and elucidated an efficient catalysis mechanism to produce xylooligosaccharides (XOSs). Codon optimization and recruitment of natural propeptide in Pichia pastoris resulted in achievement of Taxy11 activity to 1405.65 ± 51.24 U/mL. Analysis of action mode reveals that Taxy11 requires at least three xylose (Xylotriose) residues for hydrolysis to yield xylobiose. Results of site-directed mutagenesis indicate that residues Glu119, Glu210, and Asp53 of Taxy11 are key catalytic sites, while Asp203 plays an auxiliary role. The novel mechanism whereby Taxy11 catalyzes conversion of xylan or XOSs into major product xylobiose involves transglycosylation of xylose to Xylotriose or xylotetraose as substrate, to form xylotetraose or xylopentaose intermediate, respectively. Taxy11 displayed highly hydrolytic activity toward corncob xylan, producing 50.44 % of xylobiose within 0.5 h. This work provides a cost-effective and sustainable way to produce value-added biomolecules XOSs (xylobiose-enriched) from agricultural waste.
Production and accumulation of 4-O-methyl-α-D-glucuronosyl-xylotriose by growing culture of thermophilic Anoxybacillus sp. strain JT-12
Appl Biochem Biotechnol 2012 Apr;166(7):1791-800.PMID:22350872DOI:10.1007/s12010-012-9590-2.
A thermophilic Anoxybacillus sp. strain JT-12, isolated from soil, produced acidic Xylotriose, 4-O-methyl-α-D-glucuronosyl-xylotriose (MeGlcAX₃), as a main product from birchwood xylan and accumulated them in the culture under optimum conditions at pH 7.0 and 55 °C using 0.75% (w/v) birchwood xylan as a carbon source for 42-72 h. The acidic Xylotriose was purified by ethanol precipitation and high-performance liquid chromatography using NH₂ Lichosher® 100 column. The results of electrospray ionization mass spectrometry, mass to charge ratio (m/z) 603.23, confirmed that the purified sample was acidic Xylotriose that had benefits and applications in many fields.
An integrated process to produce bio-ethanol and xylooligosaccharides rich in xylobiose and Xylotriose from high ash content waste wheat straw
Bioresour Technol 2017 Oct;241:228-235.PMID:28570888DOI:10.1016/j.biortech.2017.05.109.
A bio-refinery process of wheat straw pulping solid residue (waste wheat straw, WWS) was established by combining prewashing and liquid hot water pretreatment (LHWP). The results showed that employing a prewashing step prior to the LHWP remarkably improved enzymatic glucose yields from 39.7% to 76.6%. Moreover, after 96h simultaneous saccharification and fermentation (SSF), identical ethanol yields of 0.41g/g-cellulose were obtained despite varied solid loadings (5-30%). Beyond ethanol, enzymatic post-hydrolysis of the prehydrolyzate effectively increased xylobiose and Xylotriose yields from 15mg/g-WWS and 14mg/g-WWS to 53mg/g-WWS and 20mg/g-WWS, respectively. For mass balance, about 10.9tons raw WWS will be consumed to produce 1ton ethanol, in addition to producing 614.8kg xylooligosaccharides (XOS) containing 334.3kg xylobiose and 124.8kg Xylotriose. The results demonstrated that the integrated process for the WWS bio-refinery is promising, based on value-adding co-production in addition to robust ethanol yields.