2'-Deoxyguanosine monohydrate
(Synonyms: 2'-脱氧鸟苷一水合物) 目录号 : GC38191
A purine nucleoside with diverse biological activities
Cas No.:312693-72-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2'-Deoxyguanosine is a purine nucleoside with diverse biological activities.1,2,3,4 It inhibits the clonogenic growth of HL-60 and K562 leukemia cells (IC50s = 80 and 100 ?M, respectively).1 2'-Deoxyguanosine inhibits the growth of MOLT-4 T cells and MGL-8 B cells by 99.8 and 68.3%, respectively, when used at a concentration of 50 ?M.2 It increases the number of binucleated cells, a marker of inhibited cytokinesis, in A. sativum meristems.3 2'-Deoxyguanosine (>1 ?M) also induces relaxation of precontracted isolated bovine lingual artery.4
1.Ross, D.D., Akman, S.A., Schrecker, A.W., et al.Effects of deoxynucleosides on cultured human leukemia cell growth and deoxynucleotide poolsCancer Res.41(11 Pt 1)4493-4498(1981) 2.Mitchell, B.S., Mejias, E., Daddona, P.E., et al.Purinogenic immunodeficiency diseases: Selective toxicity of deoxyribonucleosides for T cellsProc. Natl. Acad. Sci. USA75(10)5011-5014(1978) 3.Brulfert, A., Clain, E., and Deysson, G.Deoxyguanosine, a potent cytokinesis inhibitor in plant cellsExperientia30(9)1010-1011(1974) 4.Scholar, E.M.Effect of deoxypurine and deoxypyrimidine compounds on the vascular responsiveness of bovine coronary and lingual arteriesJ. Pharm. Pharmacol.32(10)726(1980)
| Cas No. | 312693-72-4 | SDF | |
| 别名 | 2'-脱氧鸟苷一水合物 | ||
| Canonical SMILES | OC[C@@H]1[C@H](C[C@H](N2C=NC3=C2N=C(N)NC3=O)O1)O.O | ||
| 分子式 | C10H15N5O5 | 分子量 | 285.26 |
| 溶解度 | DMSO : 25 mg/mL (87.64 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5056 mL | 17.5279 mL | 35.0557 mL |
| 5 mM | 0.7011 mL | 3.5056 mL | 7.0111 mL |
| 10 mM | 0.3506 mL | 1.7528 mL | 3.5056 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Short communication: Nutrient consumption patterns of Lactobacillus acidophilus KLDS 1.0738 in controlled pH batch fermentations
J Dairy Sci 2017 Jul;100(7):5188-5194.PMID:28501405DOI:10.3168/jds.2017-12607.
This work focused on elucidating the nutrient consumption patterns of Lactobacillus acidophilus to guide the design of media for high-cell-density culture. We investigated the nutrient consumption patterns of L. acidophilus KLDS 1.0738 in chemically defined media in controlled pH batch fermentations. The most abundantly consumed amino acids, vitamins, ions, and purines and pyrimidines were Glu and Gly, pyridoxine and nicotinamide, K+ and PO43-, and guanine and uracil, respectively. The highest consumption rates for amino acids, vitamins, ions, and purines and pyrimidines were Asp and Arg, folic acid and pyridoxine, Fe2+ and Mn2+, and uracil and thymine, respectively. Furthermore, most of the amino acids, as well as guanine, thymine, pyridoxine, folic acid, nicotinamide, Mg2+, PO43-, and K+ had the highest bioavailability from the end of the lag growth phase to the mid-exponential growth phase. The overall consumption of glucose, adenine nucleotides, 2'-Deoxyguanosine monohydrate, calcium pantothenate, Fe2+ and Mn2+ decreased with increasing average growth rate, indicating more effective use of these nutritional components at a higher average growth rate, as biomass yield based on nutritional component consumption increased. Our findings help to formulate complex media for high-cell-density cultivation and provide a theoretical basis for L. acidophilus feeding strategies.