Alizarin Red S sodium
(Synonyms: 茜素红S,ARS sodium) 目录号 : GC38233
Alizarin Red S sodium (Alizarin red S mono sodiumsalt, ARS sodium, C.I. Mordant Red 3, C.I 58005, Alizarin Carmine, Alizarin Red, Alizarin sodium monosulfonate, Alizarin sulfonate sodium, Alizarinsulfonic acid sodium salt, Sodium alizarinsulfonate) is an anthraquinone dye that has been used to evaluate calcium-rich deposits by cells in culture.
Cas No.:130-22-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Alizarin Red S sodium (Alizarin red S mono sodiumsalt, ARS sodium, C.I. Mordant Red 3, C.I 58005, Alizarin Carmine, Alizarin Red, Alizarin sodium monosulfonate, Alizarin sulfonate sodium, Alizarinsulfonic acid sodium salt, Sodium alizarinsulfonate) is an anthraquinone dye that has been used to evaluate calcium-rich deposits by cells in culture.
[1] Carl A Gregory, et al. Anal Biochem. 2004 Jun 1;329(1):77-84.
| Cas No. | 130-22-3 | SDF | |
| 别名 | 茜素红S,ARS sodium | ||
| Canonical SMILES | O=S(C(C=C1C2=O)=C(O)C(O)=C1C(C3=C2C=CC=C3)=O)([O-])=O.[Na+] | ||
| 分子式 | C14H7NaO7S | 分子量 | 342.26 |
| 溶解度 | Water: 10 mg/mL (29.22 mM); DMSO: < 1 mg/mL (insoluble or slightly soluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9218 mL | 14.6088 mL | 29.2176 mL |
| 5 mM | 0.5844 mL | 2.9218 mL | 5.8435 mL |
| 10 mM | 0.2922 mL | 1.4609 mL | 2.9218 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Enhancing the photo-efficacy of an organic visible-light-activated chromophore (Alizarin Red S) on zinc oxide with a Ag-Na electrolyte to photo-transform aromatic and aliphatic alcohols
RSC Adv 2019 Aug 5;9(42):24259-24266.PMID:35527857DOI:10.1039/c9ra03474a.
The development of an aqueous silver-sodium/alizarin red sensitised zinc oxide system has been reported to oxidise a range of both aromatic and aliphatic alcohols to aldehydes. Furthermore, photoluminescence spectroscopy validated the electron quenching effect of zinc oxide's defect sites after surface sensitising the metal-oxide with alizarin red. Powder diffuse reflectance UV/Vis data further substantiated the visible-light attenuated properties of alizarin red sensitised zinc oxide, and hence justification for its visible light reactivity towards alcohol oxidations. Lastly, density functional theory calculations supported the intermolecular photo-electronic transfer between alizarin red organic and zinc oxide.
Use of Alizarin Red S for histochemical staining of Ca2+ in the mouse; some parameters of the chemical reaction in vitro
Acta Anat (Basel) 1982;114(3):268-80.PMID:7158284DOI:10.1159/000145596.
Alizarin Red S (ARS) is used for histological characterization of calcium. In order to improve the conditions of the use of the dye on biological materials, the chemical reaction between ARS and Ca2+ is studied in vitro. In aqueous solution ARS and Ca2+ ions precipitate to form brick-red deposits. For precipitation to occur a neutral pH (4 less than pH less than 8) is required; the stoichiometric ratio is 1:1. ARS reacts with calcium via its sulfonate and hydroxyl groups. The apparent solubility product is around 10(-7) Na+ and K+ ions do not interfere with equilibrium of the precipitation reaction but Mg2+ and soluble proteins such as bovine serum albumin react with ARS and form soluble complexes which can lead to a decrease in the ARS quantity available to react with the calcium ions.
Nbr1-regulated autophagy in Lactoferrin-induced osteoblastic differentiation
Biosci Biotechnol Biochem 2020 Jun;84(6):1191-1200.PMID:32141386DOI:10.1080/09168451.2020.1737505.
The molecular mechanism of autophagy in Lactoferrin (LF) induced osteoblast differentiation is not fully demonstrated. In this study, alkaline phosphatase (ALP) activity, Alizarin Red S staining and ELISA were used to study N-terminal propeptide of type I procollagen (PINP) expression. mRFP-GFP-LC3 adenoviruses, mono-dansylcadaverine (MDC) staining, scanning electron microscopy, and western blot analysis was employed to probe the LF induced autophagy. The interaction between autophagy receptor Neighbor of Brca1 gene (Nbr1) and pp38 was studied. 3-methyladenine (3-MA) and chloroquine (CQ) could inhibit the activity of ALP, PINP and the autophagy in LF group. LF treatment could up-regulate and down-regulate the expressions of pp38 and Nbr1with a dose-dependent manner, respectively. LF could inhibit the recognition of pp38 and Nbr1. In addition, LF can prompt Nbr1-medicated autophagy and prevent pp38 degradation by autophagy. LF can induce Nbr1-mediated autophagy and inhibit pp38 entering into autophagy flux in the physiological process of osteoblast differentiation.Abbreviations: CQ:chloroquine；LF: Lactoferrin; 3-MA: 3-methyladenine; ALP: Alkaline phosphatase; ANOVA: Analysis of variance; CCK-8: Cell Counting Kit-8; LC3: Microtubule-associated protein light chain3; MDC: Monodansylcadaverine; Nbr1: neighbor of Brca1 gene; PINP: N-terminal propeptide of type I procollagen; PVDF: Polychlorotrifluoroethylene; pp38: phosphorylation p38; RAPA: Rapamycin; SDS: sodium dodecyl sulfate.
3D printing of Ti3C2-MXene-incorporated composite scaffolds for accelerated bone regeneration
Biomed Mater 2022 Apr 1;17(3).PMID:35316803DOI:10.1088/1748-605X/ac5ffe.
Grafting of bone-substitute biomaterials plays a vital role in the reconstruction of bone defects. However, the design of bioscaffolds with osteoinductive agents and biomimetic structures for regeneration of critical-sized bone defects is difficult. Ti3C2MXene-belonging to a new class of 2D nanomaterials-exhibits excellent biocompatibility, and antibacterial properties, and promotes osteogenesis. However, its application in preparing 3D-printed tissue-engineered bone scaffolds for repairing bone defects has not been explored. In this work, Ti3C2MXene was incorporated into composite scaffolds composed of hydroxyapatite and sodium alginate via extrusion-based 3D printing to evaluate its potential in bone regeneration. MXene composite scaffolds were fabricated and characterized by SEM, XPS, mechanical properties and porosity. The biocompatibility and osteoinductivity of MXene composite scaffolds were evaluated by cell adhesion, cell counting kit-8 test, quantitative real-time polymerase chain reaction, alkaline phosphatase activity and Alizarin Red S tests of bone mesenchymal stem cells (BMSCs). A rat calvarial defect model was performed to explore the osteogenic activity of the MXene composite scaffoldsin vivo. The results showed the obtained scaffold had a uniform structure, macropore morphology, and high mechanical strength.In vitroexperimental results revealed that the scaffold exhibited excellent biocompatibility with BMSCs, promoted cell proliferation, upregulated osteogenic gene expression, enhanced alkaline phosphatase activity, and promoted mineralized-nodule formation. The experimental results confirmed that the scaffold effectively promoted bone regeneration in a model of critical-sized calvarial- bone-defectin vivoand promoted bone healing to a significantly greater degree than scaffolds without added Ti3C2MXene did. Conclusively, the Ti3C2MXene composite 3D-printed scaffolds are promising for clinical bone defect treatment, and the results of this study provide a theoretical basis for the development of practical applications for tissue-engineered bone scaffolds.
MiR-708-5p/Pit-1 axis mediates high phosphate-induced calcification in vascular smooth muscle cells via Wnt8b/β-catenin pathway
Kaohsiung J Med Sci 2022 Jul;38(7):653-661.PMID:35460325DOI:10.1002/kjm2.12542.
Recently, the underlying mechanism of vascular calcification (VC) has been partially elucidated. However, it is still high incidence, and no effective treatment has been found. This study aims at figuring out the underlying mechanisms of microRNA-708-5p (miR-708-5p)/sodium-phosphate transporter 1 (Pit-1) axis in high phosphate (HP)-induced VC of T/G HA-VSMCs. Alizarin Red S staining was used to evaluate calcium salt deposition, and the activity of alkaline phosphatase (ALP) was determined by measuring the absorbance at 405 nm. RT-qPCR and Western blot were performed to assess the levels of miR-708-5p and Pit-1, the levels of ALP, Pit-1, β-catenin, glycogen synthesis kinase 3 β (GSK3β), and p-GSK3β proteins, respectively. The interaction between miR-708-5p and Pit-1 was validated by luciferase reporter assay. Our findings illustrated that miR-708-5p was downregulated and Pit-1was upregulated in HP-induced VC. MiR-708-5p mimics inhibited HP-induced VC. Further experiments demonstrated that miR-708-5p targets Pit-1. In addition, miR-708-5p inactivates the Wnt8b/β-catenin pathway via targeting Pit-1 to reduce HP-induced VC. MiR-708-5p has a crucial effect on VC via targeting Pit-1 and inhibiting Wnt8b/β-catenin pathway, it may serve as a new target for VC treatment.