Indole-3-acetamide
(Synonyms: 3-吲哚乙酰胺) 目录号 : GC38268
An endogenous metabolite of tryptophan in phytopathogenic bacteria
Cas No.:879-37-8
Sample solution is provided at 25 µL, 10mM.
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Indole-3-acetamide is an endogenous metabolite of tryptophan and intermediate in the biosynthesis of the major plant, fungal, and bacterial auxin hormone, indole-3-acetic acid , in phytopathogenic bacteria.1 It is formed directly from tryptophan in plants by tryptophan monooxygenase or, indirectly, through indole-3-acetonitrile or indole-3-acetaldoxime intermediates. Exogenous application of indole-3-acetamide (20 ?M) increases the expression of ami1, the gene encoding amidase 1, the enzyme that catalyzes the synthesis of indole-3-acetic acid from indole-3-acetamide, in Arabidopsis.2 It reduces relative primary root elongation in Arabidopsis mutants that have increased amidase activity and lower indole-3-acetamide levels, but not in wild-type Arabidopsis, when used at concentrations of 1 and 10 ?M. Indole-3-acetamide inhibits mouse and rat liver, as well as P. fluorescens and tryptophan 2,3-dioxygenase but not rabbit intestine or mouse epididymis indoleamine 2,3-dioxygenase.3
1.Duca, D., Lorv, J., Patten, C.L., et al.Indole-3-acetic acid in plant-microbe interactionsAnton. Van. Lee. J. M. S.106(1)85-125(2014) 2.Pérez-Alonso, M.-M., Ortiz-García, P., Moya-Cuevas, J., et al.Endogenous indole-3-acetamide levels contribute to the crosstalk between auxin and abscisic acid, and trigger plant stress responses in ArabidopsisJ. Exp. Bot.72(2)459-475(2021) 3.Eguchi, N., Watanabe, Y., Kawanishi, K., et al.Inhibition of indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase by β-carboline and indole derivativesArch. Biochem. Biophys.232(2)602-609(1984)
| Cas No. | 879-37-8 | SDF | |
| 别名 | 3-吲哚乙酰胺 | ||
| Canonical SMILES | C1=CC=CC2=C1C(=C[NH]2)CC(N)=O | ||
| 分子式 | C10H10N2O | 分子量 | 174.2 |
| 溶解度 | DMSO : 100 mg/mL (574.05 mM; Need ultrasonic); H2O : 1.3 mg/mL (7.46 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL |
| 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL |
| 10 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet