L-Methionine sulfoxide
(Synonyms: L-蛋氨酸亚砜,H-Met(O)-OH) 目录号 : GC38320
L-Methionine sulfoxide (H-Met(O)-OH)，甲硫氨酸的代谢产物，诱导 M1/classical 巨噬细胞极化，并调节氧化应激和嘌呤能的信号传递参数。
Cas No.:3226-65-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L-Methionine sulfoxide (H-Met(O)-OH), a metabolite of Methionine, induces M1/classical macrophage polarization, and modulates oxidative stress and purinergic signaling parameters[1].
[1]. Dos Santos LM, et al. Methionine and methionine sulfoxide treatment induces M1/classical macrophage polarization and modulates oxidative stress and purinergic signaling parameters. Mol Cell Biochem. 2017 Jan;424(1-2):69-78.
| Cas No. | 3226-65-1 | SDF | |
| 别名 | L-蛋氨酸亚砜,H-Met(O)-OH | ||
| Canonical SMILES | O=C(O)[C@@H](N)CCS(C)=O | ||
| 分子式 | C5H11NO3S | 分子量 | 165.21 |
| 溶解度 | H2O : 100 mg/mL (605.29 mM; Need ultrasonic); DMSO : < 1 mg/mL (insoluble or slightly soluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.0529 mL | 30.2645 mL | 60.529 mL |
| 5 mM | 1.2106 mL | 6.0529 mL | 12.1058 mL |
| 10 mM | 0.6053 mL | 3.0265 mL | 6.0529 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Utilization of L-Methionine sulfoxide, L-methionine sulfone and cysteic acid by the weanling rat
J Nutr 1976 Aug;106(8):1108-14.PMID:939991DOI:10.1093/jn/106.8.1108.
Three experiments were conducted to test the ability of weanling rats to utilize the oxidized forms of the sulfur amino acids methionine and cysteine for growth. In the first two experiments, diets were fed which contained graded levels of methionine, methionine sulfoxide and methionine sulfone. The third experiment included a comparison of two dietary levels of cysteine and cysteic acid. The 2 week weight gain and food consumption data indicated that methionine sulfoxide was utilized for growth with only 60% of the efficiency of that achieved by rats fed methionine. Methionine sulfone was not utilized for growth. Analysis of plasma sulfur amino acids showed that the rat has a limited capacity to utilize methionine sulfoxide by effecting its reduction to methionine. Cysteic acid did not support weight gain. This amino acid appeared to be rapidly catabolized to taurine. It was concluded that methionine sulfone and cysteic acid cannot be utilized by the weanling rat. Methionine sulfoxide cannot fully meet the dietary requirement of the rat methionine because of its limited capacity to reduce this amino acid.
Effects of equimolar doses of L-methionine, D-methionine and L-methionine-dl-sulfoxide on plasma and urinary amino acid levels in normal adult humans
J Nutr 1986 Jul;116(7):1185-92.PMID:3746457DOI:10.1093/jn/116.7.1185.
Plasma and urinary amino acid levels were measured in four normal adult subject administered equimolar quantities (0.0605 mmol/kg body wt) of L-methionine, D-methionine and L-methionine-dl-sulfoxide in a randomized crossover design. Plasma total methionine concentrations increased significantly (P less than 0.05) over base line (3.7 +/- 1.2 mumol/dl; mean +/- SD) after loading with each compound. Mean peak plasma methionine levels were 9.8 +/- 1.1, 14.4 +/- 2.3 and 5.2 +/- 1.0 mumol/dl after loading with L-methionine, D-methionine and L-Methionine sulfoxide, respectively. D-Methionine accounted for the increased plasma levels seen after D-methionine loading. None of the three compounds affected plasma cystine, cysteine or taurine concentrations. Plasma methionine sulfoxide concentrations were not affected by loading with D- or L-methionine but increased significantly after ingestion of L-Methionine sulfoxide. Urinary methionine excretion was 20 times higher after ingestion of D-methionine than after ingestion of L-methionine or L-Methionine sulfoxide, with the increase due to D-methionine excretion. Urinary excretion of methionine sulfoxide and its N-acetyl derivatives was not significantly higher after loading with methionine sulfoxide. The data indicate that adult humans do not utilize D-methionine efficiently as a methionine source but probably do utilize L-methionine-dl-sulfoxide.
Stereospecific electrophoretically mediated microanalysis assay for methionine sulfoxide reductase enzymes
Anal Bioanal Chem 2014 Feb;406(6):1723-9.PMID:24424966DOI:10.1007/s00216-013-7596-4.
An electrophoretically mediated microanalysis assay (EMMA) for the determination of the stereoselective reduction of L-Methionine sulfoxide diastereomers by methionine sulfoxide reductase enzymes was developed using fluorenylmethyloxycarbonyl (Fmoc)-L-methionine sulfoxide as substrate. The separation of the diastereomers of Fmoc-L-methionine sulfoxide and the product Fmoc-L-methionine was achieved in a successive multiple ionic-polymer layer-coated capillary using a 50 mM Tris buffer, pH 8.0, containing 30 mM sodium dodecyl sulfate as background electrolyte and an applied voltage of 25 kV. 4-Aminobenzoic acid was employed as internal standard. An injection sequence of incubation buffer, enzyme, substrate, enzyme, and incubation buffer was selected. The assay was optimized with regard to mixing time and mixing voltage and subsequently applied for the analysis of stereoselective reduction of Fmoc-L-methionine-(S)-sulfoxide by human methionine sulfoxide reductase A and of the Fmoc-L-methionine-(R)-sulfoxide by human methionine sulfoxide reductase B. The Michaelis-Menten constant, K m, and the maximum velocity, v max, were determined. Essentially identical data were determined by the electrophoretically mediated microanalysis assay and the analysis of the samples by CE upon offline incubation. Furthermore, it was shown for the first time that Fmoc-methionine-(R)-sulfoxide is a substrate of human methionine sulfoxide reductase B.