Chrodrimanin B
(Synonyms: Thailandolide B) 目录号 : GC45408A fungal metabolite
Cas No.:132196-54-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
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Chrodrimanin B is a meroterpenoid fungal metabolite that has been found in Talaromyces species.1 It has insecticidal activity against third instar silkworm larvae (LD50 = 10 μg/g of diet). Chrodrimanin B selectively inhibits the silkworm GABA receptor RDL (IC50 = 1.13 nM) over human α1β2γ2 subunit-containing GABA receptors (IC50 = 1.48 μM).2 The structure of thailandolide B, originally described as a C-9 epimer of chrodrimanin B, was revised in 2012 and is identical to that of chrodrimanin B.1
References
1. Hayashi, H., Oka, Y., Kai, K., et al. A new meroterpenoid, chrodrimanin C, from YO-2 of Talaromyces sp. Biosci. Biotech. Biochem. 76(4), 745-748 (2012).
2. Xu, Y., Furutani, S., Ihara, M., et al. Meroterpenoid chrodrimanins are selective and potent blockers of insect GABA-gated chloride channels. PLoS One 10(4), e0122629 (2015).
Cas No. | 132196-54-4 | SDF | |
别名 | Thailandolide B | ||
Canonical SMILES | O=C(C)O[C@H]1[C@@H](C)OC(C2=C1C3=C(C=C2O)O[C@@]([C@@]4([H])C3)(C)[C@@H](O)C[C@@]([C@]4(C)C=C5)([H])C(C)(C)C5=O)=O | ||
分子式 | C27H32O8 | 分子量 | 484.5 |
溶解度 | DMSO: Soluble,Ethanol: 1 mg/ml,Methanol: Soluble | 储存条件 | Store at -20°C |
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10 mM | 0.2064 mL | 1.032 mL | 2.064 mL |
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Competitive Chrodrimanin B interactions with rat brain GABAA receptors revealed by radioligand binding assays
Pestic Biochem Physiol 2022 May;183:105074.PMID:35430068DOI:10.1016/j.pestbp.2022.105074.
Meroterpenoid compounds chrodrimanins produced by Talaromyces sp. YO-2 have been shown to act as competitive antagonists of silkworm larval GABAA receptors using electrophysiology, yet no further evidence has been provided to support such an action. We have investigated the actions of Chrodrimanin B on rat brain GABAA receptors by binding assays with non-competitive ligand of GABAA receptors [3H]EBOB and competitive ligands [3H]gabazine and [3H]muscimol. Chrodrimanin B did not significantly affect the binding of [3H]EBOB while reducing the binding of [3H]gabazine and [3H]muscimol to the rat membrane preparations. Chrodrimanin B increased the dissociation constant Kd of [3H]gabazine and [3H]muscimol without significantly affecting the maximum binding, pointing to competitive interactions of Chrodrimanin B with rat GABAA receptors in support of our previous observation that the compound acts as a competitive antagonist on the silkworm larval GABA receptor.
Elucidation and Heterologous Reconstitution of Chrodrimanin B Biosynthesis
Org Lett 2018 Dec 7;20(23):7504-7508.PMID:30417647DOI:10.1021/acs.orglett.8b03268.
The biosynthetic gene cluster of the fungal meroterpenoid Chrodrimanin B (4) was discovered in Penicillium verruculosum TPU1311, and the complete biosynthetic pathway of 4 has been elucidated by heterologous reconstitution of its biosynthesis in Aspergillus oryzae, as well as by in vitro characterizations of selected enzymes. The present study has identified the polyketide synthase that produces 6-hydroxymellein (3) and also provided a biosynthetic platform of chrodrimanins for further bioengineering.
Meroterpenoid Chrodrimanins Are Selective and Potent Blockers of Insect GABA-Gated Chloride Channels
PLoS One 2015 Apr 22;10(4):e0122629.PMID:25902139DOI:10.1371/journal.pone.0122629.
Meroterpenoid chrodrimanins, produced from Talaromyces sp. YO-2, are known to paralyze silkworm (Bombyx mori) larvae, but their target is unknown. We have investigated the actions of Chrodrimanin B on ligand-gated ion channels of silkworm larval neurons using patch-clamp electrophysiology. Chrodrimanin B had no effect on membrane currents when tested alone at 1 μM. However, it completely blocked the γ-aminobutyric acid (GABA)-induced current and showed less pronounced actions on acetylcholine- and L-glutamate-induced currents, when delivered at 1 μM for 1 min prior to co-application with transmitter GABA. Thus, chrodrimanins were also tested on a wild-type isoform of the B. mori GABA receptor (GABAR) RDL using two-electrode voltage-clamp electrophysiology. Chrodrimanin B attenuated the peak current amplitude of the GABA response of RDL with an IC50 of 1.66 nM. The order of the GABAR-blocking potency of chrodrimanins B > D > A was in accordance with their reported insecticidal potency. Chrodrimanin B had no open channel blocking action when tested at 3 nM on the GABA response of RDL. Co-application with 3 nM Chrodrimanin B shifted the GABA concentration response curve to a higher concentration and further increase of Chrodrimanin B concentration to 10 nM; it reduced maximum current amplitude of the GABA response, pointing to a high-affinity competitive action and a lower affinity non-competitive action. The A282S;T286V double mutation of RDL, which impairs the actions of fipronil, hardly affected the blocking action of Chrodrimanin B, indicating a binding site of Chrodrimanin B distinct from that of fipronil. Chrodrimanin B showed approximately 1,000-fold lower blocking action on human α1β2γ2 GABAR compared to RDL and thus is a selective blocker of insect GABARs.
Tafuketide, a phylogeny-guided discovery of a new polyketide from Talaromyces funiculosus Salicorn 58
Appl Microbiol Biotechnol 2016 Jun;100(12):5323-38.PMID:26810200DOI:10.1007/s00253-016-7311-4.
A phylogeny-guided approach was applied to screen endophytic fungi containing type I polyketide synthase (PKS I) biosynthetic gene sequences and aimed to correlate genotype to chemotype for the discovery of novel bioactive polyketides. Salicorn 58, which was identified as Talaromyces funiculosus based on its internal transcribed spacer (ITS) and ribosomal large-subunit (LSU) DNA sequences, showed significant target bands. A chemical investigation of the culture of Salicorn 58 was allowed for the isolation of a new polyketide, Talafun (1), and a new natural product, N-(2'-hydroxy-3'-octadecenoyl)-9-methyl-4,8-sphingadienin (2), together with six known compounds, including chrodrimanin A (3), Chrodrimanin B (4), N-(4-hydroxy-2-methoxyphenyl) acetamide (5), butyl β-glucose (6), 3β,15β-dihydroxyl-(22E, 24R)-ergosta-5,8(14),22-trien-7-dione (7), and (3β,5a,8a,22E)-5,8-epidioxyergosta-6,22-dien-3-ol (8). Their chemical structures were elucidated by extensive spectroscopic analysis and electro circular dichroism (ECD) spectrum calculations. Antioxidant experiments revealed that compound 5 showed strong ABTS(+) radical scavenging activity with an IC50 value of 11.43 ± 1.61 μM and potent ferric reducing activity (FRAP assay) with FRAP value of 187.52 ± 2.97. Antimicrobial assays revealed that compounds 1 and 4 showed high levels of selectivity toward Escherichia coli with MIC values of 18 ± 0.40 and 43 ± 0.52 μM, respectively. Compounds 2 and 3 exhibited broad-spectrum antimicrobial activity against Staphylococcus aureus, Mycobacterium smegmatis, Micrococcus tetragenus, Mycobacterium phlei, and E. coli, respectively. The results from the current research highlight the advantage of phylogeny-guided pipeline for the screening of new polyketides from endophytic fungi containing PKS I genes.