Diprotin A
(Synonyms: 抑二肽素A,Ile-Pro-Ile) 目录号 : GC45436A tripeptide inhibitor of DPP-4
Cas No.:90614-48-5
Sample solution is provided at 25 µL, 10mM.
Diprotin A is a tripeptide inhibitor of dipeptidyl peptidase 4 (DPP-4; IC50 = 1.1 μg/ml).1 It inhibits degradation of glucagon-like peptide 1 in culture with insulin-secreting BRIN-BD11 rat pancreatic β-cells when used at a concentration of 25 μM.2 Diprotin A (5 mg/ml) pre-incubation enhances chemotaxis of murine embryonic stem cells towards stromal cell-derived factor-1 (SDF-1/CXCL12) in vitro.3 Pre-incubation of CD34+ human umbilical cord blood cells with diprotin A (5 mM) prior to injection increases engraftment in NOD/SCID recipient mice.4
References
1. Umezawa, H., Aoyagi, T., Ogawa, K., et al. Diprotins A and B, inhibitors of dipeptidyl aminopeptidase IV, produced by bacteria. J. Antibiot. (Tokyo) 37(4), 422-425 (1984).
2. Green, B.D., Liu, H.K., McCluskey, J.T., et al. Function of a long-term, GLP-1-treated, insulin-secreting cell line is improved by preventing DPP IV-mediated degradation of GLP-1. Diabetes Obes. Metab. 7(5), 563-569 (2005).
3. Guo, Y., Hangoc, G., Bian, H., et al. SDF-1/CXCL12 enhances survival and chemotaxis of murine embryonic stem cells and production of primitive and definitive hematopoietic progenitor cells. Stem Cells 23(9), 1324-1332 (2005).
4. Campbell, T.B., Hangoc, G., Liu, Y., et al. Inhibition of CD26 in human cord blood CD34+ cells enhances their engraftment of nonobese diabetic/severe combined immunodeficiency mice. Stem Cells Dev. 16(3), 347-354 (2007).
Cas No. | 90614-48-5 | SDF | |
别名 | 抑二肽素A,Ile-Pro-Ile | ||
Canonical SMILES | O=C([C@@H](N)[C@@H](C)CC)N1CCC[C@H]1C(N[C@H](C(O)=O)[C@@H](C)CC)=O | ||
分子式 | C17H31N3O4 | 分子量 | 341.5 |
溶解度 | DMF: 33 mg/ml,DMSO: 25 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.9283 mL | 14.6413 mL | 29.2826 mL |
5 mM | 0.5857 mL | 2.9283 mL | 5.8565 mL |
10 mM | 0.2928 mL | 1.4641 mL | 2.9283 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet