GCSF Rat
目录号 : GP20324Granulocyte-Colony Stimulating Factor Rat Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 97.0% as determined by:(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Granulocyte colony stimulating factor; Protein Csf3; Csf3. | ||
| Amino Acid Sequence | KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI. | ||
| Solubility | It is recommended to reconstitute the lyophilized GCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of > 2.0б┴ 107 IU/mg. | ||
| Formulation | Lyophilized from a 0.2µm filtered solution in 5mM Sodium Citrate, pH 4.0. | ||
GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes.
The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of > 2.0б┴ 107 IU/mg.
Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.