Germicidin B
目录号 : GC16571
An autoregulative inhibitor of spore germination
Cas No.:150973-78-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Germicidin is a pyranone antibiotic originally excreted by Streptomyces viridochromogenes NRRL B-1551. Germicidin has an inhibitory effect on the germination of its own arthrospores at a concentration as low as 200 pM (40 pg/ml). Higher concentrations of germicidin inhibited porcine Na+/K+-activated ATPase and retarded the germination of the cress Lepidium sativum with an ID50 of 100 μM. Germicidin was the first known autoregulative inhibitor of spore germination in the genus Streptomyces and was isolated from the supernatant of germinated spores, but also from the supernatant of the submerged culture [1].
Germicidins B inhibited germination of S. coelicolor A3(2) spores above 1 μg/ml. The S. coelicolor A3(2) spores collected from a single petri dish (9 cm i.d.) contained 5.4 μg of germicidin A (~2.7 × 10-14 g per spore) and contents of germicidins B, C and D were 0.2–0.8 μg. The activity of the spore extract corresponded well with the sum of the activity of each germicidin. Germicidins functioned as self-germination inhibitors in S. coelicolor A3(2) [2].
References:
[1] PETERSEN F, ZHNER H, METZGER J W, et al. Germicidin, an autoregulative germination inhibitor of Streptomyces viridochromogenes NRRL B-1551[J]. The Journal of antibiotics, 1993, 46(7): 1126-1138.
[2] Aoki Y, Matsumoto D, Kawaide H, et al. Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3 (2)[J]. The Journal of antibiotics, 2011, 64(9): 607-611.
| Cas No. | 150973-78-7 | SDF | |
| 化学名 | 3-ethyl-4-hydroxy-6-(1-methylethyl)-2H-pyran-2-one | ||
| Canonical SMILES | O=C1OC(C(C)C)=CC(O)=C1CC | ||
| 分子式 | C10H14O3 | 分子量 | 182.2 |
| 溶解度 | DMF: Soluble,DMSO: Soluble,Ethanol: Soluble,Methanol: Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.4885 mL | 27.4424 mL | 54.8847 mL |
| 5 mM | 1.0977 mL | 5.4885 mL | 10.9769 mL |
| 10 mM | 0.5488 mL | 2.7442 mL | 5.4885 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。