PC DBCO-PEG4-NHS ester

PC DBCO-PEG4-NHS ester is a PEG based PROTAC linker that can be used as a linker in click chemistry for the synthesis of PROTAC molecules. The reaction between the DBCO group in PC DBCO-PEG4-NHS ester and azide compounds (usually azide methane (CH2N2) or azide amide analogues) belongs to the stress promoted azide alkyne cycloaddition reaction (SPAAC), which is a metal free chemical reaction. The alkyne portion of DBCO can bind with azide groups and form a stable 1,2,3-triazole cyclobutane compound through a [3+2] cycloaddition mechanism.

PC DBCO-PEG4-NHS ester can be used for surface modification of samples such as peptides, proteins, enzymes, and live cells, to prepare protein peptide conjugates, protein/peptide oligonucleotide conjugates, and antibody enzyme/antibody drug conjugates. PC DBCO-PEG4-NHS ester only reacts with azide compounds, exhibiting good bioorthogonality, high selectivity, and high reaction rate. Due to the absence of metal catalysts in the reaction, this product is also suitable for use in living organisms or other metal sensitive environments, and has broad application prospects and scientific value.

It is recommended to explore the labeling ratio of PC DBCO-PEG4-NHS ester to ligand at around 5:1 or 8:1, and label at room temperature for 30-1h. The usage conditions need to be adjusted according to specific experimental needs and labeling effects.

PC DBCO-PEG4-NHS ester是一种PEG类PROTAC linker，在点击化学中可作为连接剂用于合成PROTAC分子。PC DBCO-PEG4-NHS ester中的DBCO基团与叠氮化合物（通常是叠氮甲烷（CH2N2）或者叠氮酰胺类似物）的反应属于应力促发叠氮-炔烃环加成反应（Strain-Promoted Azide-Alkyne Cycloaddition，SPAAC），是一种无金属催化的化学反应。DBCO的炔烃部分能够与叠氮基团结合，通过[3+2]环加成机制形成一个稳定的1,2,3-三唑环丁烷化合物。

PC DBCO-PEG4-NHS ester能够用于多肽、蛋白、酶、活细胞等样本的表面修饰，制备蛋白-多肽偶联物、蛋白/多肽-寡核苷酸偶联物以及抗体-酶/抗体药物偶联物。PC DBCO-PEG4-NHS ester仅与叠氮化合物发生反应，具有良好的生物正交性、高选择性和高反应速率。由于反应无需金属催化剂，本产品也适用于生物体内或其他对金属敏感的环境中，具有广泛的应用前景和科学价值。

PC DBCO-PEG4-NHS ester与配体的标记比例建议在5:1或8:1左右摸索，室温标记30min-1h，需要根据具体实验需求及标记效果调整使用条件。