Ghrelin (rat)
(Synonyms: 生长激素释放肽) 目录号 : GC11583
Ghrelin (rat) 是一种生长激素释放肽，于 1999 年首次在大鼠胃中发现，是生长激素促分泌素受体 的配体。
Cas No.:258338-12-4
Sample solution is provided at 25 µL, 10mM.
Ghrelin (rat), a growth hormone-releasing peptide first discovered in rat stomach in 1999, is a ligand for the growth hormone secretagogue receptor[1，2].
Retroperitoneal (RP) adipocytes were cultured in the absence or presence of either Ghrelin (rat) or desacyl Ghrelin (rat) and in combination with either inhibitors of protein synthesis, insulin, dexamethasone (DXM), or GHSR1a antagonist. The results indicate that both Ghrelin (rat) forms possess a direct leptin-releasing activity (LRA) on RP adipocytes and significantly enhanced adipocyte ob mRNA expression[4].
Ghrelin (rat) and the GHS-R1α receptor antagonist GHRP-6 were injected intravenously in rats followed by blood flow measurements using a microsphere technique. Ghrelin (rat) decreased, while GHRP-6 in fasted, but not fed, rats selectively increased islet blood flow fourfold[3]. The initial electrophysiological results displayed that ex vivo administration of Ghrelin (rat) increased NAc shell output in brain slices from drug- and training-na?ve rats. In rats with an acquired skilled reach performance, acute as well as repeated treatment with a Ghrelin (rat) receptor (GHSR-1 A) antagonist decreased the number of sucrose pellets consumed[5]. Ghrelin (rat) administration attenuated sepsis symptoms induced by CLP. Blood flow in the stomach greater curvature was significantly higher in the CLP induced sepsis group rats compared with the sham operation group, whereas there was no difference between the CLP group treated with Ghrelin (rat) and the sham rats. Ghrelin (rat) administration also reduced the secretion of pro inflammatory cytokines compared with the CLP induced sepsis group rats[6]. Ghrelin (rat) signaling increases the reward from social interaction in a manner that reflects the degree of divergence in body weight between the social pair[7].
References:
[1]. Tokudome T, Kangawa K. Physiological significance of ghrelin in the cardiovascular system. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(8):459-467. doi: 10.2183/pjab.95.032. PMID: 31611501; PMCID: PMC6819151.
[2]. Kojima M, Hosoda H, et,al.in is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999 Dec 9;402(6762):656-60. doi: 10.1038/45230. PMID: 10604470.
[3]. Drott CJ, Franzén P, et,al. Ghrelin in rat pancreatic islets decreases islet blood flow. Am J Physiol Endocrinol Metab. 2019 Jul 1;317(1):E139-E146. doi: 10.1152/ajpendo.00004.2019. Epub 2019 May 7. PMID: 31063397.
[4]. Giovambattista A, Gaillard RC, et,al.Ghrelin gene-related peptides modulate rat white adiposity. Vitam Horm. 2008;77:171-205. doi: 10.1016/S0083-6729(06)77008-X. PMID: 17983857.
[5]. Vestlund J, Bergquist F, et,al. Ghrelin signalling within the rat nucleus accumbens and skilled reach foraging. Psychoneuroendocrinology. 2019 Aug;106:183-194. doi: 10.1016/j.psyneuen.2019.04.008. Epub 2019 Apr 8. PMID: 30999229.
[6]. Li B, Lin Q, et,al.Ghrelin regulates sepsis?induced rat acute gastric injury. Mol Med Rep. 2019 Jun;19(6):5424-5432. doi: 10.3892/mmr.2019.10208. Epub 2019 Apr 30. PMID: 31059095; PMCID: PMC6522907.
[7]. Schéle E, Pfabigan DM, et,al. Ghrelin Induces Place Preference for Social Interaction in the Larger Peer of a Male Rat Pair. Neuroscience. 2020 Nov 1;447:148-154. doi: 10.1016/j.neuroscience.2020.01.027. Epub 2020 Feb 5. PMID: 32032669.
Ghrelin (rat) 是一种生长激素释放肽，于 1999 年首次在大鼠胃中发现，是生长激素促分泌素受体[1，2] 的配体。
腹膜后 (RP) 脂肪细胞在生长素释放肽（大鼠）或去酰基生长素释放肽（大鼠）不存在或存在的情况下进行培养，并结合蛋白质合成抑制剂、胰岛素、地塞米松 (DXM) 或 GHSR1a 拮抗剂。结果表明，两种Ghrelin（大鼠）形式均对RP脂肪细胞具有直接的瘦素释放活性（LRA），并显着增强脂肪细胞ob mRNA的表达[4]。
在大鼠体内静脉注射 Ghrelin（大鼠）和 GHS-R1α 受体拮抗剂 GHRP-6，然后使用微球技术测量血流量。 Ghrelin（大鼠）减少，而 GHRP-6 在禁食但未进食的大鼠中选择性地使胰岛血流量增加四倍[3]。最初的电生理学结果表明，Ghrelin（大鼠）的离体给药增加了未接受药物和训练的大鼠脑切片中的 NAc 壳层输出。在具有后天熟练触及能力的大鼠中，用 Ghrelin（大鼠）受体 (GHSR-1A) 拮抗剂进行急性和重复治疗可减少消耗的蔗糖颗粒数量[5]。 Ghrelin（大鼠）给药减轻了 CLP 引起的败血症症状。与假手术组相比，CLP 引起的脓毒症组大鼠胃大弯的血流量显着高于假手术组，而 Ghrelin（大鼠）治疗的 CLP 组与假手术大鼠之间没有差异。与 CLP 诱导的脓毒症组大鼠相比，Ghrelin（大鼠）给药也减少了促炎细胞因子的分泌[6]。生长素释放肽（大鼠）信号以一种反映社交对之间体重差异程度的方式增加社交互动的奖励[7]。
| Cell experiment [1]: | |
|
Cell lines |
RP adipocytes |
|
Preparation Method |
After isolation, adipocytes were seeded (800 µl of suspension per well) onto 24 well plates; then, 200 µl of the following test solutions were added: culture medium either alone (basal) or containing insulin; dexamethasone phosphate, Ghrelin (rat)(0.001-1 nM), desacyl Ghrelin (rat), cycloheximid, actinomycin D , Ghrelin (rat) antagonist or, when appropriate, different combinations of these substances. |
|
Reaction Conditions |
0.001-1 nM for 0.5-48h at 37°C |
|
Applications |
Retroperitoneal (RP) adipocytes were cultured in the absence or presence of either Ghrelin (rat) or desacyl Ghrelin (rat) and in combination with either inhibitors of protein synthesis, insulin, dexamethasone (DXM), or GHSR1a antagonist. The results indicate that both Ghrelin (rat) forms possess a direct leptin-releasing activity (LRA) on RP adipocytes and significantly enhanced adipocyte ob mRNA expression. |
| Animal experiment [2]: | |
|
Animal models |
Male Sprague-Dawley rats (M&B, Ry, Denmark) 12-16 wk of age and weighing ~300 g |
|
Preparation Method |
Ghrelin (rat) (100 nmol/kg) dissolved in saline, was injected into the catheterized femoral vein. The influence of both exogenously administered Ghrelin (rat) and antagonism of endogenous Ghrelin (rat) levels (by GHRP-6 administration) was investigated in normal-fed rats. |
|
Dosage form |
100 nmol/kg Ghrelin (rat)(I.V) |
|
Applications |
Ghrelin (rat) and the GHS-R1α receptor antagonist GHRP-6 were injected intravenously in rats followed by blood flow measurements using a microsphere technique. Ghrelin decreased, while GHRP-6 in fasted, but not fed, rats selectively increased islet blood flow fourfold. |
|
References: [1]. Giovambattista A, Gaillard RC, et,al. Ghrelin gene-related peptides modulate rat white adiposity. Vitam Horm. 2008;77:171-205. doi: 10.1016/S0083-6729(06)77008-X. PMID: 17983857. |
|
| Cas No. | 258338-12-4 | SDF | |
| 别名 | 生长激素释放肽 | ||
| 化学名 | (4S,5Z,7S,8Z,10S,11Z,13S,14Z,16S,17Z,19S,20Z,22S,23Z,25S,26Z,28S,29Z,31S)-28-((1H-imidazol-5-yl)methyl)-4-((Z)-(((S,Z)-1-(((S,Z)-6-amino-1-(((S)-6-amino-1-((S)-2-((S)-2-((1Z,3S,4Z,6S,7Z,9S,10Z,12S)-6-(4-aminobutyl)-12-((S)-2-((Z)-(((S)-1-carboxy-4-guanidi | ||
| Canonical SMILES | CCCCCCCC(OC[C@@](/N=C(O)/[C@](/N=C(O)/CN)([H])CO)([H])/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](C(N1CCC[C@@]1([H])/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/ | ||
| 分子式 | C147H245N45O42 | 分子量 | 3314.83 |
| 溶解度 | Soluble to 1 mg/ml in Water | 储存条件 | Desiccate at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.3017 mL | 1.5084 mL | 3.0167 mL |
| 5 mM | 0.0603 mL | 0.3017 mL | 0.6033 mL |
| 10 mM | 0.0302 mL | 0.1508 mL | 0.3017 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet