Gibberellin A1
(Synonyms: 赤霉素) 目录号 : GC49215
A plant hormone with growth stimulatory activities
Cas No.:545-97-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >96.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Gibberellin A1 is a plant hormone that has been found in Phaseolus multiflorus and has growth stimulatory activities.1,2,3 It reverses inhibition of spinach stem elongation induced by the plant growth retardants AMO-1618 or BX-112 .2 Gibberellin A1 increases hypocotyl, but not radicle, growth of cucumber seedlings when used at concentrations of 30 and 100 µM.3
1.MacMillan, J., Seaton, J.C., and Suter, P.J.Plant hormones—I: Isolation of gibberellin A1 and gibberellin A5 from Phaseolus multiflorusTetrahedron11(1-2)60-66(1960) 2.Zeevaart, J.A., Gage, D.A., and Talon, M.Gibberellin A1 is required for stem elongation in spinachProc. Natl. Acad. Sci. USA90(15)7401-7405(1993) 3.Halevy, A.H., and Cathey, H.M.Effects of structure and concentration of gibberellins on the growth of cucumber seedlingsBot. Gaz.122(1)63-67(1960)
| Cas No. | 545-97-1 | SDF | |
| 别名 | 赤霉素 | ||
| Canonical SMILES | OC([C@H]1[C@@]2([H])[C@]3(OC([C@@]2([C@H](CC3)O)C)=O)[C@@]4([H])[C@]15C[C@@](CC4)(C(C5)=C)O)=O | ||
| 分子式 | C19H24O6 | 分子量 | 348.4 |
| 溶解度 | Acetone:Water (1:1): soluble,DMSO: soluble,Methanol: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8703 mL | 14.3513 mL | 28.7026 mL |
| 5 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL |
| 10 mM | 0.287 mL | 1.4351 mL | 2.8703 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Gibberellin A1 metabolism contributes to the control of photoperiod-mediated tuberization in potato
PLoS One 2011;6(9):e24458.PMID:21961036DOI:10.1371/journal.pone.0024458.
Some potato species require a short-day (SD) photoperiod for tuberization, a process that is negatively affected by gibberellins (GAs). Here we report the isolation of StGA3ox2, a gene encoding a GA 3-oxidase, whose expression is increased in the aerial parts and is repressed in the stolons after transfer of photoperiod-dependent potato plants to SD conditions. Over-expression of StGA3ox2 under control of constitutive or leaf-specific promoters results in taller plants which, in contrast to StGA20ox1 over-expressers previously reported, tuberize earlier under SD conditions than the controls. By contrast, StGA3ox2 tuber-specific over-expression results in non-elongated plants with slightly delayed tuber induction. Together, our experiments support that StGA3ox2 expression and gibberellin metabolism significantly contribute to the tuberization time in strictly photoperiod-dependent potato plants.
Gibberellin A1 is required for stem elongation in spinach
Proc Natl Acad Sci U S A 1993 Aug 1;90(15):7401-5.PMID:11607418DOI:10.1073/pnas.90.15.7401.
The effects of the growth retardants 2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate (AMO-1618) and calcium 3,5-dioxo-4-propionylcyclohexanecarboxylate (BX-112) on stem elongation were investigated in the rosette plant spinach (Spinacia oleracea L.) under long-day (LD) conditions. Stem growth induced by a LD treatment was prevented by both retardants. The inhibition caused by AMO-1618 was reversed by Gibberellin A1 (GA1) and GA20, whereas the effects of BX-112 were reversed by GA1 only. Six GAs (GA53, GA44, GA19, GA20, GA1, and GA8) were quantified by gas chromatography-selected ion monitoring using internal standards. Plants treated with BX-112 had reduced levels of GA1 and GA8 and accumulated GA53, GA44, GA19, and GA20. The relative levels of four additional GAs (3-epi-GA1, GA29, GA60, and GA81) were compared by ion intensities only. Relative to GA81, the level of GA29 was decreased by BX-112, whereas the levels of GA60 and 3-epi-GA1 were increased. Transfer of spinach from short-day conditions to LD conditions caused an increase in all identified GAs of the early 13-hydroxylation pathway with GA20, GA1, and GA8 showing the largest increases. These findings support the position that, of the GAs belonging to the early 13-hydroxylation pathway, GA1 is the primary GA active per se for stem elongation in spinach. The increase in endogenous GA1 in plants in LD conditions is most likely the primary factor for stem elongation.
The conversion of Gibberellin A1 to gibberellin A 8 by a cell-free enzyme system
Planta 1974 Sep;119(3):183-91.PMID:24442496DOI:10.1007/BF00429043.
An enzyme system which catalyzes hydroxylation of (3)[H]Gibberellin A1 ([(3)H]GA1) to [(3)H]GA8 was obtained from homogenates of germinating bean (Phaseolus vulgaris L.) seed. The system remained in the supernatant after centrifugation at 95000×g for 2 h. Hydroxylation occurred at one of the specifically (3)H-labeled positions (C-2) and, concomitantly, produced [(3)H]water whic was used to measure formation of [(3)H]GA8. NADPH and/or ascorbate are cofactors utilized by this enzyme. EDTA effectively inhibited the system, suggesting a cation requirement.
The Metabolism of Gibberellin A20 to Gibberellin A1 by Tall and Dwarf Mutants of Oryza sativa and Arabidopsis thaliana
Plant Physiol 1994 Dec;106(4):1367-1372.PMID:12232414DOI:10.1104/pp.106.4.1367.
The purpose of this study was to demonstrate the metabolism of gibberellin A20 (GA20) to Gibberellin A1 (GA1) by tall and mutant shoots of rice (Oryza sativa L.) and Arabidopsis thaliana (L.) Heynh. The data show that the tall and dx mutant of rice and the tall and ga5 mutant of Arabidopsis metabolize GA20 to GA1. The data also show that the dy mutant of rice and the ga4 mutant of Arabidopsis block the metabolism of GA20 to GA1. [17-13C,3H]GA20 was fed to tall and the dwarf mutants, dx and dy, of rice and tall and the dwarf mutants, ga5 and ga4, of Arabidopsis. The metabolites were analyzed by high-performance liquid chromatography and full-scan gas chromatography-mass spectrometry together with Kovats retention index data. For rice, the metabolite [13C]GA, was identified from tall and dx seedlings; [13C]GA1 was not identified from the dy seedlings. [13C]GA29 was identified from tall, dx, and dy seedlings. For Arabidopsis, the metabolite [13C]GA1 was identified from tall, ga5, and ga4 plants. The amount of [13C]GA1 from ga4 plants was less than 15% of that obtained from tall and ga5 plants. [13C]GA29 was identified from tall, ga5, and ga4 plants. [13C]GA5 and [13C]GA3 were not identified from any of the six types of plant material.
Mass production of pure Gibberellin A1 by Phaeosphaeria sp. L487 and the fungal preparation of [U-13C]Gibberellin A1
Biosci Biotechnol Biochem 2001 Sep;65(9):2095-7.PMID:11676028DOI:10.1271/bbb.65.2095.
The mass production of pure Gibberellin A1 (GA1) by shake-culturing Phaeosphaeria sp. L487 was investigated. Its GA1 production was markedly influenced by natural nitrogen sources and NH4NO3. When the fungus was cultured in an 8% glucose-1.5% oatmeal-0.1% NH4NO3-0.5% KH2PO4-0.1% MgSO4 x 7H2O medium for 3 weeks, the amount of GA1 in the culture filtrate was up to ca. 200 microg/ml: the addition of safflower oil to the culture medium two weeks after inoculation prolonged the GA1-production period to produce 300 microg/ml. Further preparation of [U-13C]GA, as a tool for the analysis of a complex of GA1 and its binding protein was attempted by using the fungus. The fungal culture in a [U-13C]glucose-oatmeal medium gave 6 mg of crystalline 13C-enriched GA1. Its 13C-enrichment of ca. 75% and 1J(CC) values were determined by NMR spectrometry.