GLK E.coli
目录号 : GP22524Glucokinase E.coli Recombinant
Sample solution is provided at 25 µL, 10mM.
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GLK E.coli Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 344 amino acids (1-321 a.a) andhaving a molecular mass of 37.1kDa.GLK is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| Purity | Greater than 95% as determined by SDS-PAGE. | Source | E.coli. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Glucokinase; Glucose kinase; glk; b2388; JW2385. | ||
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRVYLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLIQFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADNRLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGFRAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | GLK protein solution (1.0 mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl and 10% glycerol. | ||
Glucokinase also known as GLK is a member of the bacterial glucokinasefamily. GLK is not highly significant in E.coli since glucose is already transported into the cell throughthe PTS system as glucose 6-phosphate.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.