Glp-Amyloid-β (3-40) Peptide (human) (trifluoroacetate salt)
(Synonyms: AβN3(pE)-40, AβpE3-40, Glp-FRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV) 目录号 : GC90298一段β淀粉样肽的片段
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Glp-Amyloid-β (3-40) peptide is a fragment of amyloid-β (1-40) that contains the cyclic amino acid pyroglutamate at the N-terminus. Glp-Amyloid-β (3-40) peptide is a major component of the endogenous amyloid-β content in post-mortem brain tissue from patients with Alzheimer’s disease.1 It is formed through cleavage of the N-terminal aspartate and alanine on Aβ40 and Aβ42 followed by dedration of glutamic acid by glutaminyl cyclase.2 Glp-Amyloid-β (3-40) peptide has been used as a reference peptide for the quantification of total pyroglutamate amyloid-β in APP23 mouse brain.1
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.2424 mL | 1.2119 mL | 2.4238 mL |
5 mM | 0.0485 mL | 0.2424 mL | 0.4848 mL |
10 mM | 0.0242 mL | 0.1212 mL | 0.2424 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。