(L)-Sodium lactate
(Synonyms: L-乳酸钠, Sodium L-Lactate) 目录号 : GC20111
L-乳酸钠（(L)-Sodium lactate）是一种源自L-乳酸的有机酸，在无氧糖酵解过程中由丙酮酸通过乳酸盐脱氢酶产生。
Cas No.:867-56-1
Sample solution is provided at 25 µL, 10mM.
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(L)-Sodium lactate is an organic acid derived from L-lactic acid, which is produced from pyruvate by lactate dehydrogenase during anaerobic glycolysis[1]. (L)-Sodium lactate helps with pH regulation, acid-base balance and various physiological processes, and can be used as an antimicrobial agent, preservative, buffer and stabilizer, cell culture medium supplement and cellular fuel source[2]. (L)-Sodium lactate has neuroprotective effects and can prevent glutamate excitotoxicity[3].
In vitro, (L)-Sodium lactate (5, 10, 25 mM) treatment of human SH-SY5Y cell line and astrocytes for 4h and 24h dose-dependently increased the expression of heat shock protein 70 kDa (HSP70), brain-derived neurotrophic factor (BDNF) and inducible nitric oxide synthase (iNOS) in both cell lines, and also increased the release of nitric oxide (NO) through the Griess reaction [4]. Treatment of U937 cells with (L)-sodium lactate (0-12 mM) for 24 h enhanced the secretion of matrix metalloproteinase-1 (MMP-1), cytokines (IL-1β and IL-6), and the transcriptional activity of activator protein 1 (AP-1) and nuclear factor (NF-κB)[5].
In vivo, (L)-sodium lactate (2g/kg/day) was intraperitoneally injected into myocardial infarction (MI) mice for 14 days, which reduced cardiomyocyte apoptosis, inhibited cardiac fibrosis, and increased the density of neovascularization after MI, and increased the expression of arginine-1 (Arg-1) protein in the heart[6].
References:
[1] Dhup S, Kumar Dadhich R, Ettore Porporato P, et al. Multiple biological activities of lactic acid in cancer: influences on tumor growth, angiogenesis and metastasis[J]. Current pharmaceutical design, 2012, 18(10): 1319-1330.
[2] Langa R L S. Optimisation of cell growth and shelf life stability of Megasphaera elsdenii NCIMB 41125[M]. University of Pretoria (South Africa), 2010.
[3] Castillo X, Rosafio K, Wyss M T, et al. A probable dual mode of action for both L-and D-lactate neuroprotection in cerebral ischemia[J]. Journal of Cerebral Blood Flow & Metabolism, 2015, 35(10): 1561-1569.
[4] Coco M, Caggia S, Musumeci G, et al. Sodium L‐lactate differently affects brain‐derived neurothrophic factor, inducible nitric oxide synthase, and heat shock protein 70 kDa production in human astrocytes and SH‐SY5Y cultures[J]. Journal of neuroscience research, 2013, 91(2): 313-320.
[5] Nareika A, He L, Game B A, et al. Sodium lactate increases LPS-stimulated MMP and cytokine expression in U937 histiocytes by enhancing AP-1 and NF-κB transcriptional activities[J]. American Journal of Physiology-Endocrinology and Metabolism, 2005, 289(4): E534-E542.
[6] Zhang J, Huang F, Chen L, et al. Sodium lactate accelerates M2 macrophage polarization and improves cardiac function after myocardial infarction in mice[J]. Cardiovascular therapeutics, 2021, 2021(1): 5530541.
L-乳酸钠（(L)-Sodium lactate）是一种源自L-乳酸的有机酸，在无氧糖酵解过程中由丙酮酸通过乳酸盐脱氢酶产生[1]。(L)-Sodium lactate有助于pH调节、酸碱平衡和各种生理过程，可以用作抗菌剂、防腐剂、缓冲剂和稳定剂，细胞培养基补充剂和细胞燃料来源[2]。(L)-Sodium lactate具有神经保护作用，可以防止谷氨酸兴奋性毒性[3]。
在体外，(L)-Sodium lactate（5, 10, 25 mM）处理人类SH-SY5Y细胞系和星形胶质细胞4h和24h，剂量依赖性地增加了两种细胞的热休克蛋白70 kDa（HSP70）、脑源性神经营养因子（BDNF）和诱导型一氧化氮合酶（iNOS）的表达，还增加了通过格里斯反应释放的一氧化氮（NO[4]。(L)-Sodium lactate（0-12 mM）处理U937细胞24h，增强了基质金属蛋白酶-1（MMP-1）、细胞因子（IL-1β和IL-6）的分泌，增强了激活蛋白1（AP-1）和核转录因子（NF-κB）的转录活性[5]。
在体内，(L)-Sodium lactate（2g/kg/day）通过腹腔注射治疗心肌梗死（MI）小鼠14天，减少了心肌细胞凋亡、抑制心脏纤维化并增加心肌梗死后新生血管密度，增加了心脏中精氨酸-1（Arg-1）蛋白的表达[6]。
| Cell experiment [1]: | |
Cell lines | Human SH-SY5Y cell line、astrocyte |
Preparation Method | Cells were stimulated or not (untreated controls) with different concentrations of (L)-Sodium lactate (5, 10, and 25 mM) for 4 and 24 h. |
Reaction Conditions | 5, 10, 25 mM; 4, 24 h |
Applications | SH-SY5Y cells respond with a dose-dependent increase of heat shock protein 70 kDa (HSP70) to treatment with (L)-Sodium lactate at 4 as well as at 24 h. However, astrocytes produced a significantly greater amount of HSP70 (an increase of approximately twofold). |
| Animal experiment [2]: | |
Animal models | Male C57BL/6 mice |
Preparation Method | Male C57BL/6 mice aged 8 to 10 weeks were anaesthetized with 3% isoflurane. Then, mice were placed in the right lateral decubitus position. With the aid of surgical microscope, the left anterior descending artery (LAD) could be visualized and permanently ligated with a 7-0 silk suture at the site of 2 mm below the left appendix. (L)-Sodium lactate (2g/kg/day) or saline was treated intraperitoneally one day after operation. On day 3 or day 14 after treatment with sodium lactate or saline, myocardial infarction (MI) mice were anaesthetized and the hearts were exposed. |
Dosage form | 2g/kg/day; i.p. |
Applications | (L)-Sodium lactate decreased cardiomyocyte apoptosis, restrained cardiac fibrosis, and increased new vessel density post-MI. |
References: [1]Coco M, Caggia S, Musumeci G, et al. Sodium L‐lactate differently affects brain‐derived neurothrophic factor, inducible nitric oxide synthase, and heat shock protein 70 kDa production in human astrocytes and SH‐SY5Y cultures[J]. Journal of neuroscience research, 2013, 91(2): 313-320. [2]Zhang J, Huang F, Chen L, et al. Sodium lactate accelerates M2 macrophage polarization and improves cardiac function after myocardial infarction in mice[J]. Cardiovascular therapeutics, 2021, 2021(1): 5530541. | |
| Cas No. | 867-56-1 | SDF | |
| 别名 | L-乳酸钠, Sodium L-Lactate | ||
| 分子式 | C3H5NaO3 | 分子量 | 112.06 |
| 溶解度 | DMSO: 196.3 mM | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 8.9238 mL | 44.619 mL | 89.2379 mL |
| 5 mM | 1.7848 mL | 8.9238 mL | 17.8476 mL |
| 10 mM | 0.8924 mL | 4.4619 mL | 8.9238 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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